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Titlebook: Axonal Transport; Methods and Protocol Alessio Vagnoni Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive license t

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發(fā)表于 2025-3-21 18:22:49 | 只看該作者 |倒序瀏覽 |閱讀模式
期刊全稱Axonal Transport
期刊簡稱Methods and Protocol
影響因子2023Alessio Vagnoni
視頻videohttp://file.papertrans.cn/168/167747/167747.mp4
發(fā)行地址Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
學(xué)科分類Methods in Molecular Biology
圖書封面Titlebook: Axonal Transport; Methods and Protocol Alessio Vagnoni Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive license t
影響因子.This volume covers a wide range of model systems from invertebrate to humans and .in vitro?.and?.in vivo.. ?Chapters detail methods on squid, aplysia, xenopus, mouse in vivo, ex vivo, rodent primary neurons, human-derived neurons, zebrafish, drosophila in vivo, primary neurons, c. elegans, and in vitro reconstitution assays.Written in the format of the highly successful?.Methods in Molecular Biology?.series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols..?..Authoritative and cutting-edge,?.Axonal Transport: Methods and Protocols?.aims?to?further our understanding of the trafficking of?cellular?components in the nervous system leading to the advancement of basic knowledge that underlines the cell biology of the?neuron..The chapters “?Live imaging of RNA Transport and Translation in .Xenopus. Retinal Axons,?? ?Retrograde Axonal Transport of Neurotrophins in Basal Forebrain Cholinergic Neurons,?? ?Assessment of Mitochondrial Trafficking as A Surrogate of Fast Axonal Transport in Human Induced Pluripotent Stem Cell Derived Spinal Motor Neu
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Cancer Drug Discovery and Developmentaptic strength. This transport is impaired in several neurodegenerative disorders such as Alzheimer’s disease. Thus, it is critical to understand the regulation and underlying mechanisms of the transport process. . provides a powerful experimental system for studying the interplay between synaptic a
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發(fā)表于 2025-3-22 03:19:16 | 只看該作者
Cancer Drug Discovery and Developmentdge of the molecular mechanisms underlying axonal mRNA translation has rapidly expanded. However, tools to study mRNA localization and translation in real time with high spatial precision were not available until recently. Here, we present a live imaging approach to examine axonal mRNA trafficking a
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發(fā)表于 2025-3-22 06:27:59 | 只看該作者
Hypoxia and Reactive Oxygen Specieser to remain healthy. This process, known as axonal transport, can be imaged in real time through epifluorescent imaging of fluorescently labeled proteins, organelles, and other cargoes. While this is most conveniently done in primary neuronal cultures, it is more physiologically relevant when carri
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Hypoxia in the Northern Gulf of Mexicol and functional integrity. Cargoes are transported along the microtubule (MT) network whose post-translational modifications influence transport dynamics. Here, we describe methods to modulate MT acetylation and record its impact on axonal transport in cultured mouse cortical projection neurons as
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