Titlebook: Axon Growth and Regeneration; Methods and Protocol Andrew J. Murray Book 2014 Springer Science+Business Media New York 2014 Axon growth.Axo

密碼

Analysis of Calcium Signals in Steering Neuronal Growth Cones In Vitroion often functions as the polarizing signal during guided migration including axon guidance. In this chapter, we describe a protocol to quantitatively monitor the spatiotemporal dynamics of calcium signals in neuronal growth cones in the presence of an extracellular concentration gradient of axon guidance cue.

改良

In Vivo Electroporation of Adult Mouse Sensory Neurons for Studying Peripheral Axon Regenerationos. Here we report a rapid and efficient approach to transfect adult mouse dorsal root ganglion neurons in vivo with precise spatiotemporal control via electroporation. This approach will allow both gain- and loss-of-function experiments in vivo to study the function of adult sensory neurons, such as sensory axon regeneration.

隱語

Axon Growth and Regeneration978-1-4939-0777-9Series ISSN 1064-3745 Series E-ISSN 1940-6029

尖叫

Milan Gopal,Tariq Abbas,J. L. Pippi Salleion often functions as the polarizing signal during guided migration including axon guidance. In this chapter, we describe a protocol to quantitatively monitor the spatiotemporal dynamics of calcium signals in neuronal growth cones in the presence of an extracellular concentration gradient of axon guidance cue.

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minimal

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oxidant

否認(rèn)

延期

1064-3745 ctions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls..Practical and r978-1-4939-4584-9978-1-4939-0777-9Series ISSN 1064-3745 Series E-ISSN 1940-6029