Titlebook: Awake Behaving Mesoscopic Brain Imaging; James E. Niemeyer,Hongtao Ma,Theodore H. Schwartz Book 2025 The Editor(s) (if applicable) and The

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Technical Considerations for Widefield Optical Imaging During Visuomotor Behaviorsaviors are deemed as involving coordinated neural activity across brain areas, including the visual and motor system. Widefield optical imaging is a valuable tool for studying coordinated neural activity across these areas at the mesoscale. However, visuomotor behaviors can cause spurious imaging si

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Mesoscale Ca++ Imaging of Seizures and Interictal Activity in Models of Chronic Epilepsyl activity) is also often abnormal in epilepsy, but the relationship between these two phenomena is not well understood. In this chapter, we describe a method for imaging brain activity across the surface of the dorsal cortex in mouse models of epilepsy using Ca. indicators, as well as how to analyz

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Mesoscale Imaging of Neural Dynamics in Epilepsy- and long-range connections to local and widespread interconnected brain networks. Recent advances in neuroimaging of activity-dependent fluorophores have made widefield imaging an ideal tool for tracking the neural dynamics underlying the onset and propagation of seizures. Here we describe the met

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Technical Considerations for Widefield Optical Imaging During Visuomotor Behaviorsield imaging during visuomotor behaviors, including recording and visual-stimulation systems. Subheading . discusses technical challenges that often arise during widefield imaging of visuomotor behaviors, including hemodynamic and mechanical artifacts that can distort the optical signal. We discuss

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Mesoscale Ca2+ Imaging During Locomotiontions between regions. A similar approach is needed for other brain regions involved in controlling locomotion such as the cerebellar cortex. With the development of new generations of genetically encoded Ca. indicators and improved tools for optical imaging, mesoscale Ca. imaging provides a powerfu

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