Titlebook: Atherosclerosis; Experimental Methods Angela F. Drew Book 2001 Humana Press 2001

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Genetically Manipulated Models of Atherosclerosis in Mice,sis The focus of this chapter is genetically manipulated models (. Chapter 1 for discussion regarding diet-induced atherosclerosis). For a complex genetic disease like atherosclerosis, mouse models provide a suitable means for studying large numbers of animals and a means for manipulating genes thou

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Lipoprotein Isolation and Analysis from Serum by Preparative Ultracentrifugation,t of waterinsoluble nutrients through the vascular and extravascular fluids from their site of synthesis or absorption to peripheral tissues (.,.). These hydrophobic nutrients (triacylglycerols [TGs] and cholesteryl esters [CEs]) are delivered from the liver and intestine to other tissues in the bod

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Separation of Plasma Lipoproteins in Self-Generated Gradients of Iodixanol,e characterized on the basis of differences in density and charge (.). Centrifugation is the ‘gold-standard’ for the analysis of plasma lipo-protein classes and beta quantitation, and other analytical procedures (.–.). Lipoprotein classes are separated by flotation of plasma or serum in a series of

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9 Assay for Serum Glycated Lipoproteins,d oxidized LDL are the only modified lipoproteins that exist naturally in the human body, but oxidized LDL does not exist in the circulation because of the presence of many antioxidizing agents (.). LDL cannot be oxidized until it has entered the arterial wall from the circulation. The only modified

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10 Lipoprotein(a) Quantitation,as an independent risk factor for the development of coronary heart disease (.). During the last two decades the structure and composition of Lp(a) has been investigated using different physicochemical and biochemical methods (.,.). These studies revealed that Lp(a) resembles the cholesterol-rich lo

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