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Titlebook: Applications of Monolithic Column and Isotope Dimethylation Labeling in Shotgun Proteome Analysis; Fangjun Wang Book 2014 Springer-Verlag

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Fangjun WangNominated by Chinese Academy of Sciences as an outstanding PhD thesis.Describes the first example of online multidimensional chromatography separation using capillary monolithic columns.Introduces the
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Springer Theseshttp://image.papertrans.cn/a/image/159511.jpg
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Online Sample Injection and Multidimensional Chromatography Separation by Using Strong-Cation Exchater equilibrium the adsorbed peptides are eluted from the trap column to a reversed phase (RP) capillary column for LC separation. There are usually two types of instrument configurations for the automated sample injection in nanoflow LC-MS/MS analyses. In the first type of configuration, the trap c
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Development of Polymer-Based Hydrophobic Monolithic Columns and Their Applications in Proteome Analporous structure, which makes them good alternatives to packed columns in chromatography separation. Especially in shotgun proteome analysis, where capillary column (usually 10–100?μm i.d.) is adopted to increase the sensitivity as well as extremely complex protein samples such as serum is inevitabl
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Large-Scale Proteome and Phosphoproteome Quantification by Using Dimethylation Isotope Labeling,ich makes the protein sample even more complex. The occupancy level of a PTM on specific site of a protein is also critical to the biological function of the protein in the regulation of different physiological and pathological processes, such as the protein phosphorylation is related to the signal
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