Titlebook: Applications of Genome Modulation and Editing; Paul John Verma,Huseyin Sumer,Jun Liu Book 2022 The Editor(s) (if applicable) and The Autho

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978-1-0716-2303-9The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines

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Gérard Duhaime,Sébastien Lévesqueected into mouse embryos at different preimplantation stages. Subsequently, simple DNA plasmids were used to microinject into the pronuclei of fertilized mouse oocytes and that method became the reference for many years. The isolation of embryonic stem cells together with advances in genetics allowe

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Gérard Duhaime,Sébastien Lévesquection expendable, the available BAC libraries are a precious source for life science. Their comprising of extended genomic regions provides an ideal basis for creating a large targeting vector. Here, we describe the identification of suitable BACs from their libraries and their verification prior to

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https://doi.org/10.1007/978-3-031-17299-1 the biotechnology industry. In this chapter, we describe an easy to establish, robust, and practical pipeline that can be used to generate immortalized cell lines, from different tissues, to capture cell linage context and validate the tools required for genome editing and genetic modification. Thi