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Titlebook: Applications of Aminoacylation Ribozymes That Recognize the 3′-end of tRNA; Naohiro Terasaka Book 2017 Springer Japan KK, part of Springer

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發(fā)表于 2025-3-21 18:21:26 | 只看該作者 |倒序瀏覽 |閱讀模式
期刊全稱Applications of Aminoacylation Ribozymes That Recognize the 3′-end of tRNA
影響因子2023Naohiro Terasaka
視頻videohttp://file.papertrans.cn/160/159285/159285.mp4
發(fā)行地址Nominated by The University of Tokyo as an outstanding Ph.D. thesis.Provides a detailed description of the history and applications of aminoacylation ribozymes.Describes potentials of aminoacylation r
學(xué)科分類Springer Theses
圖書封面Titlebook: Applications of Aminoacylation Ribozymes That Recognize the 3′-end of tRNA;  Naohiro Terasaka Book 2017 Springer Japan KK, part of Springer
影響因子.In this thesis, applications of aminoacylation ribozymes named flexizymes are described. Flexizymes have the following unique characteristics: (i) substrate RNA is recognized by two consecutive base pairs between the 3‘-end of substrate RNA and the 3‘-end of the flexizyme; (ii) these base pairs can be substituted with other base pairs; and (iii) various activated amino acids can be used as substrates including both canonical and noncanonical amino acids. This flexible aminoacylation of RNAs by flexizymes was used to label endogenous tRNAs to be removed, and in vitro selection using the tRNA-depleted library enabled the discovery of the novel interaction between the microRNA precursor and metabolites. Flexizymes are also used to prepare various aminoacyl-tRNAs bearing mutations at the 3‘-end to engineer the translation machinery and to develop the orthogonal translation machinery. .The first part of the research demonstrated that SELEX is appropriate for discovering the interaction between small RNA and ligands, and suggested that more RNA motif binding to small molecules exists in small RNAs. The second?part opened a door to new opportunities for in vitro synthetic biology involvi
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Discovery of Human MicroRNA Precursor Binding to Folic Acid by Small RNA Transcriptomic SELEX,4, .). miRNAs are loaded into an Argonaute protein to form the RNA-induced silencing complex (RISC) and RISC binds to target mRNA via base paring with miRNA to regulate the expression of target gene. miRNAs are transcribed from genome as part of a long primary transcripts (pri-miRNAs) and then pri-m
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,Orthogonal Ribosome–tRNAs Pair by Engineering of Peptidyl Transferase Center,tson–Crick base pairs with bases of 23S rRNA in the peptidyl transferase center (PTC) in the classical state (Voorhees et al. in Nat Struct Mol Biol 16:528–533, .) and translocation (Dorner et al. in Nat Struct Mol Biol 13:234–241, .) during translation. These base pairs are important for translatio
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