Titlebook: Antiviral Resistance in Plants; Methods and Protocol Kappei Kobayashi,Masamichi Nishiguchi Book 2019 Springer Science+Business Media, LLC,

LUMEN

Ulf Melin,Karin Axelsson,Truls L?fstedtnse (HR). Such .-mediated transient gene expression methods have contributed to the identification of new plant . genes and facilitated the analysis of their functions. Here we describe a model method, by which several tobamovirus . genes from Solanaceous plants have been successfully identified and characterized molecularly.

AVANT

Kappei Kobayashi,Masamichi NishiguchiIncludes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts

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Methods in Molecular Biologyhttp://image.papertrans.cn/a/image/158710.jpg

RLS898

Identification and Functional Analysis of NB-LRR-Type Virus Resistance Genes: Overview and Functionnse (HR). Such .-mediated transient gene expression methods have contributed to the identification of new plant . genes and facilitated the analysis of their functions. Here we describe a model method, by which several tobamovirus . genes from Solanaceous plants have been successfully identified and characterized molecularly.

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小溪

Hanne H?glund Rydén,Sara Hofmann,Guri Verne but is not associated with any severe symptoms. Using this method together with inoculation of a challenge virus and transient overexpression of a viral elicitor or another host signaling component by agroinfiltration, as well as measurement of hallmarks of antiviral responses, we can address the r

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https://doi.org/10.1007/978-3-319-44421-5ide broad, durable viral resistance, as it can target conserved regions of the viral genome and can also be customized to emerging viral variants. Moreover, this method can be used in many crop species, as it requires little or no knowledge of the host plant’s genome.

Brain-Waves

Catherine G. Mkude,Maria A. Wimmerf CMV-?2b, preparation of the mutant plants for virus inoculation, identification and characterization of . mutants, and cloning of the genes responsible for the mutant phenotype by either the genetic linkage to T-DNA insertion or a mapping-by-sequencing approach.

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Lecture Notes in Computer Science molecular analysis of virus accumulation in plant tissues. This approach is aimed to easily identify artificial sRNAs with high antiviral activity that could be expressed in transgenic plants for highly durable antiviral resistance.