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Titlebook: Antibody Usage in the Lab; Laura Caponi,Paola Migliorini Book 1999 Springer-Verlag Berlin Heidelberg 1999 Antibody.Antigen.ELISA.Immunoflu

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發(fā)表于 2025-3-21 18:36:26 | 只看該作者 |倒序?yàn)g覽 |閱讀模式
期刊全稱Antibody Usage in the Lab
影響因子2023Laura Caponi,Paola Migliorini
視頻videohttp://file.papertrans.cn/159/158480/158480.mp4
發(fā)行地址First lab manual describing the most useful immunological techniques based on antibodies Easy-to-follow step-by-step instructions with troubleshooting guide Practical hints and experimental strategies
學(xué)科分類Springer Lab Manuals
圖書封面Titlebook: Antibody Usage in the Lab;  Laura Caponi,Paola Migliorini Book 1999 Springer-Verlag Berlin Heidelberg 1999 Antibody.Antigen.ELISA.Immunoflu
影響因子A handy lab manual that permits quick and easy access to the techniques commonly used to analyse antibody specificity. Some of the most useful immunological techniques based on antibodies are described, including ELISA, immunoblotting and immunoprecipitation protocols that provide useful tools for recognising immunological specificities, and basic immunofluorescence and immunohistochemistry procedures for the in situ identification of antigens. The topics are discussed from a practical point of view, describing the theoretical basis of each technique and sample protocols plus a troubleshooting guide. Attention is focused on the various aspects of the protocols described thus providing the reader with the maximum possible information on each technique.
Pindex Book 1999
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Springer Lab Manualshttp://image.papertrans.cn/a/image/158480.jpg
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Planung und Betrieb hydraulischer Anlagen,ELISA (Enzyme-Linked ImmunoSorbent Assay) is a well-known and widely used laboratory technique that is able to measure the ligands present in small amounts in biological samples. ELISA combines a high degree of sensitivity and specificity with a detection system based on a colorimetric reaction; it can also provide quantitative results.
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Hans Jürgen Matthies,Karl Theodor Reniuscal studies. The availability of these tools has facilitated the localisation of various molecules in tissues and subcellular structures using immunocytochemical techniques. Immunocytochemistry is based on the ability of antibodies to bind a tissue antigen that can subsequently be revealed using direct or indirect techniques.
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Hans Jürgen Matthies,Karl Theodor Reniusty of these methods has increased because they are precise, sensitive and relatively inexpensive. In fact, antibodies have the ability to recognize their ligands with high specificity and can also be labeled fairly easily without significant loss of binding activity. Thanks to these properties, they
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,Elemente und Ger?te zur Energieübertragung,s, it is called Western blotting. Different techniques can be used to separate the proteins contained in a sample, such as SDS-PAGE (sodium dodecyl sulphate polyacrylamide electrophoresis, see Appendix 2), which is based on differences in the molecular weight of proteins, or isoelectrofocusing, whic
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https://doi.org/10.1007/978-3-8351-9240-9s procedure is based on the separation of the antibody-bound antigen from unbound antigens by means of reagents specific for the Fc portion of the immunoglobulins, usually protein A or protein G. Different preparations of unsolubilised protein A or G are commercially available. The matrix they are c
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Hans Jürgen Matthies,Karl Theodor Reniuscal studies. The availability of these tools has facilitated the localisation of various molecules in tissues and subcellular structures using immunocytochemical techniques. Immunocytochemistry is based on the ability of antibodies to bind a tissue antigen that can subsequently be revealed using dir
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