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Titlebook: Antibody Engineering; Methods and Protocol Damien Nevoltris,Patrick Chames Book 2018Latest edition Springer Science+Business Media, LLC, pa

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發(fā)表于 2025-3-21 19:47:44 | 只看該作者 |倒序瀏覽 |閱讀模式
期刊全稱Antibody Engineering
期刊簡稱Methods and Protocol
影響因子2023Damien Nevoltris,Patrick Chames
視頻videohttp://file.papertrans.cn/159/158471/158471.mp4
發(fā)行地址Includes cutting-edge techniques for the study of antibody engineering.Provides step-by-step detail essential for reproducible results.Contains updated key implementation advice from the experts
學(xué)科分類Methods in Molecular Biology
圖書封面Titlebook: Antibody Engineering; Methods and Protocol Damien Nevoltris,Patrick Chames Book 2018Latest edition Springer Science+Business Media, LLC, pa
影響因子This detailed new edition provides complete and easy access to a variety of antibody engineering techniques.? The volume explores topics such as the generation of native, synthetic, or immune antibody libraries, the selection of lead candidates via the different powerful and innovative display technologies, Fc engineering, as well as their production, characterization, and optimization of antibodies. Written for the highly successful .Methods in Molecular Biology .series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.?.Authoritative and up-to-date, .Antibody Engineering: Methods and Protocols, Third Edition. presents the reader with an extensive toolbox to create the powerful molecules of tomorrow..
Pindex Book 2018Latest edition
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發(fā)表于 2025-3-21 23:23:17 | 只看該作者
https://doi.org/10.1007/978-3-662-02149-1gen encounter by somatically mutating their genome sequences. The end result is a series of antibodies that display higher affinities and specificities to specific antigens. This process is called affinity maturation. Recent improvements in computer hardware and modeling algorithms now enable the ra
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Einführung in die organische Chemiedescribe a simple protocol to make large and diverse libraries based on a single or a limited number of frameworks. The approach is flexible enough to be used with any antibody format, either single-chain (scFv, VHH) or multi-chain (Fv, Fab, (Fab′)2), and to target in a single step the six complemen
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Einführung in die organische Chemie a tedious multistep process that involves the generation of heavy chain as well as light chain display plasmids in different haploid yeast strains followed by yeast mating. Here, we present a focused one-step Golden Gate cloning approach for the generation of yeast surface display Fab libraries tha
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發(fā)表于 2025-3-23 04:13:19 | 只看該作者
Stickstoffhaltige Verbindungen,tic tool development. In many case, the protein of interest, or a relevant portion of this protein such as its extracellular domain, is available as purified protein. In such cases, phage display on purified antigen is an easy and fast way to select antibody fragment able to efficiently bind this an
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Einführung in die organische Chemiesystem. Approximately 38% of the entire genome codes for plasma membrane proteins; however the discovery and development of antibody binders to such targets are a technical challenge. Methods to raise binders against such targets by cloning and expressing soluble extracellular regions have been met
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