Titlebook: Antibody Engineering; Roland Kontermann,Stefan Dübel Book 20011st edition Springer-Verlag Berlin Heidelberg 2001 Amino acid.Antigen.Cell p

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書目名稱Antibody Engineering影響因子(影響力)




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MILL

Construction of scFv Fragments from Hybridoma or Spleen Cells by PCR Assembly et al. 2000), very many hybridomas have been generated, and are continuously being made, which produce monoclonal antibodies with very interesting properties. Even when naive and synthetic libraries may be a source of antibodies, often the immune response of an experimental animal may be of interes

洞穴

Construction of scFv from Hybridoma by Two-Step Cloning available from hybridoma cell lines. Here, a method is presented to obtain the genetic information for the antigen binding part of the antibody from hybridoma cells, and to assemble it into a functional bacterially expressed fusion protein (scFv fragment). To achieve this, vectors have been constru

fidelity

Dawdle

痛打

Generation of Naive Human Antibody Librariesdes and folded proteins could be displayed on the surface of filamentous bacteriophage (Smith, 1985; Bass et al., 1990). Since the generation of the first human antibodies by phage display (Winter et al., 1994), the technology has developed to the point where large scFv repertoires have been created

Exaggerate

Generation of Antibody Gene Libraries from Seropositive Human Donors however, antibodies to threatening antigens cannot be produced, as in the case of most cancer cells, or they appear too late to control the antigen, as e. g. in the case of intoxication. In this situation, therapeutic antibodies can be applied to help our own immune system. Further, therapeutic ant

乞討

Generation of Rabbit Immune Librariesat proteins provides an alternative strategy for the rapid generation of monoclonal antigen-binding proteins. Various antibody-displaying phage libraries have been described, which are based on the B cell repertoire of rearranged immunoglobulin genes from spleen and bone marrow of previously immuniz

積習(xí)已深

monologue