Titlebook: Antibody Arrays; Methods and Protocol Kelly C. Whittaker,Ruo-Pan Huang Book 2021 Springer Science+Business Media, LLC, part of Springer Nat

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Reverse Phase Protein Arrays,y on a single slide. Each RPPA slide is subsequently probed with catalyzed signal amplification chemistries and a single primary antibody, a secondary antibody, and either fluorescent or colorimetric dyes. The focus of this chapter is to describe RPPA detection and imaging using a colorimetric (diam

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Profiling Glycoproteins on Functionalized Reverse Phase Protein Array,rray (polyGPA), to measure the variation of glycosylation patterns on a three-dimensionally functionalized RPPA. Without the need of specific antibody towards glycosylation, polyGPA represents a highly sensitive strategy to analyze protein glycosylation in multiple complex biological samples in para

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978-1-0716-1066-4Springer Science+Business Media, LLC, part of Springer Nature 2021

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Kelly C. Whittaker,Ruo-Pan HuangIncludes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts