Titlebook: Animal Cell Technology: From Target to Market; Proceedings of the 1 Elisabeth Lindner-Olsson,Nathalie Chatzissavidou,E Conference proceedin

結(jié)果

Hamper

Eco-Stats: Data Analysis in Ecologyness of host cells for transient expression, the effect of over-expression of Protein Kinase Bα has been explored. In this report we demonstrate that over-expression of PKBα can improve transient recombinant protein expression 40% to >200% depending on the protein being expressed and the cell line used.

未完成

Eco-Urbanism and the South East Asian Cityg this technique means that only one round of cloning is required, resulting in time and cost savings in cell line development for therapeutic protein production. This technique has been used to clone hybridoma, CHO and NS0 cell lines.

Aerate

Neurite Outgrowth Promoting Substance from a Marine Alga, ,ls (PC12D cells) was found to be capable of responding to NGF in a faster rate. They only take 24–48 h to extend neurite after being exposed to NGF (.). Accordingly, PC12D cells are ideal for rapid screening of large quantity of samples for NGF-potentiating substance.

濕潤(rùn)

漂白

廣口瓶

Capillary-Aided Cell Cloningg this technique means that only one round of cloning is required, resulting in time and cost savings in cell line development for therapeutic protein production. This technique has been used to clone hybridoma, CHO and NS0 cell lines.

不容置疑

Anti-Tumor Activity of Noguchi Catalyser 21?, a Mineral Water Containing Natural Leaf Soilhe production of anti-tumor cytokines. RT-PCR analysis revealed that the transcription of IL-12 p40 subunit was significantly enhanced in murine macrophage cell line J774-1, RAW264 and BALB/c mouse-derived peritoneal cavity macrophage. This suggests that enhancement of the tumor immune systems via IL-12 production may be enhanced by Catalyser.

STENT

High Cell Density Cultivation of Mast Cells in Fluidized-Bed and Fixed-Bed Bioreactorsate cell density 38 times higher than in suspension batch cultivation. The immobihzed ceUs were continuously proHferating, analogous to the exponential growth phase in batch fermentation, proven by cell cycle analysis. An interleukin-3 concentration of 2 ng/ml has been found to be sufficient to support optimal cell growth.

濕潤(rùn)

Successful Transfection of Partially Purified DNA in the HEK293EBNA Cell Line for Transient Expressie-scale transient transfection for rapid recombinant protein production (.). The transfectability of a DNA extract containing considerable host cell contaminants was assessed, using the calcium phosphate method (.) and HEK293 cells. Impure plasmid DNA proved to be as transfectable as pure preparations in this widely used cell line.