Titlebook: Amebiasis; Biology and Pathogen Tomoyoshi Nozaki,Alok Bhattacharya Book 2015 Springer Japan 2015 amebiasis.entamoeba.infectious disease.mic

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The Continuously Expanding Universe of ,and host were the primary bases for naming species. We now know that both are unreliable, because host ranges can be broad and identical morphology can hide substantial genetic differences. Since Dobell, the number of accepted . species in humans has continuously increased, with the most recent bein

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Multilocus Sequence Typing System (MLST): Genetic Diversity and Genetic Components to Virulencebtained from an area in Bangladesh where amebiasis is endemic. These markers seemed to segregate autonomously and exhibit extensive haplotype diversity. Despite the genetic heterogeneity, a strong association was observed between two single-nucleotide polymorphisms (SNPs) in the cylicin-2 gene and d

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The tRNA Gene-Linked STRs and Other Genetic Typing Methodss is, therefore, what is responsible for the variable outcome of infection. To address whether it is linked to the genotype of the infecting strain, several protein-coding and noncoding genes have been investigated. However, with the use of only tRNA gene-linked short tandem repeat (STR) loci, a sig

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Genetic Manipulation Techniquesf (1) the introduction of a transfection system to express exogenous genes and to up- and downregulate the expression of selected genes, (2) the completion of the parasite’s genome sequencing, including the genomes of various . spp. and some of its strains, and (3) the development of microarrays bot

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Surveying , Transcriptome Using Massively Parallel cDNA Sequencingt promises to revolutionize transcriptomics over the next decade. In this chapter, we first review the transcriptome research of . during the past 25 years in retrospect: from cDNA library screening in the 1980s to the latest RNA-Seq analyses. Then, we share our experiences on applying RNA-Seq to th

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: Bridging the Gap Between Environmental Stress and Epigenetic Regulationate of knowledge about the . DNA/tRNA methyltransferase (Dnmt2) machinery and the related EhMLBP, a protein involved in the recognition of methylated DNA targets. The regulation of these epigenetic components by environmental challenges relevant to the biology of the parasite (including heat shock,