Titlebook: Hematologic Malignancies; Methods and Techniqu Guy B. Faguet Book 2001 Springer Science+Business Media New York 2001

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Cytogenetics Analysisphomas, must be based on the examination of the involved cells or tissues. Thus, in the case of the leukemias bone marrow (BM) aspirations yield optimal results in the preponderant number of patients, whereas in the lymphomas affected tissues, usually lymph nodes, are the best source of cells carryi

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Comparative Genomic Hybridization for the Analysis of Leukemias and Lymphomass and lymphomas. The knowledge about specific chromosomal aberrations has been an essential prerequisite for the identification of pathogenetically relevant genes. Important examples are molecular genetic analyses of the breakpoint regions in chromosomal translocations, which resulted in the detecti

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Spectral Karyotyping (SKY) of Hematologic Malignancieshis chromosomal abnormality as areciprocal translocation t(9;22)(q34;q1 1) by Rowley in 1973 (.), the identification of the genes involved at the translocation breakpoints (.,.), and ultimately the demonstration of the leukemogenic activity of the resulting fusion product (.), represent hallmarks fo

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Detecting Monoclonal Immunoglobulin andT-Cell Receptor Gene Rearrangements in B- andT-Cell Malignance immunoglobulin heavy-chain gene (.), a variable gene segment (V) is joined to a diversity gene segment (.), and then subsequently this complex is recombined to a joining gene segment (J). Nucleotides are added and removed at random at the . and . junctions (.). Similarly, early in development of T

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