| 期刊全稱 | Advanced Technologies for Protein Complex Production and Characterization | | 影響因子2023 | M. Cristina Vega | | 視頻video | http://file.papertrans.cn/147/146311/146311.mp4 | | 發(fā)行地址 | Provides in-depth coverage of expression systems tailored for the production of multisubunit complexes.Gives extensive practical advice on how to design and conduct protein complex production experime | | 學(xué)科分類 | Advances in Experimental Medicine and Biology | | 圖書封面 |  | | 影響因子 | .This book presents advanced expression technologies for the production of protein complexes. Since complexes lie at the heart of modern biology, the expression, purification, and characterization of large amounts of high-quality protein complexes is crucial for the fields of biomedicine, biotechnology, and structural biology. From co-expression in .E. coli., yeast, mammalian and insect cells to complex reconstitution from individual subunits, this book offers useful insights and guidance for successful protein expressionists...Across several sections readers will discover existing opportunities for the production of protein complexes in bacterial systems (including membrane proteins and cell-free co-expression), methylotrophic and non-methylotrophic yeasts, protozoa (.Leishmania terantolae. and .Dictyostelium discoideum.), baculovirus-infected insect cells, mammalian cells, plants and algae. Complex reconstitution from individually purified subunits or subcomplexes is discussed as a complementary strategy. A last section introduces briefly some of the biophysical and structural characterization techniques for macromolecular complexes using state-of-the-art solution scattering and | | Pindex | Book 2016 |
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