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Titlebook: AMPK; Methods and Protocol Dietbert Neumann,Benoit Viollet Book 2018 Springer Science+Business Media, LLC 2018 bacterial expression.baculov

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發(fā)表于 2025-3-21 18:42:28 | 只看該作者 |倒序?yàn)g覽 |閱讀模式
期刊全稱AMPK
期刊簡稱Methods and Protocol
影響因子2023Dietbert Neumann,Benoit Viollet
視頻videohttp://file.papertrans.cn/143/142933/142933.mp4
發(fā)行地址Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
學(xué)科分類Methods in Molecular Biology
圖書封面Titlebook: AMPK; Methods and Protocol Dietbert Neumann,Benoit Viollet Book 2018 Springer Science+Business Media, LLC 2018 bacterial expression.baculov
影響因子.This volume explores the latest methods used to study AMPK by computational, biochemical, biophysical, cellular, and ex vivo and in vivo approaches. The chapters in this book cover specific topics, such as methods to measure change in cellular energy metabolism and analyze metabolic pathways regulated by AMPK; bioinformatics tools to identify AMPK targets; knockdown of AMPK by CRISPR-Cas9; production and crystallization of full-length human AMP-activated protein kinase; cell-free assays to measure the effects of regulatory ligands on AMPK; use of sensors of AMPK activity; AMPK protein interaction by yeast two-hybrid; the role of AMPK in inflammation and autophagy; analyzing the AMPK function in C. elegans and mammals (with special focus on skeletal muscle, blood vessels, kidneys, pancreatic islets and hypothalamus); and human γ2 AMPK mutations. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Cutting edge and thorough, .AMPK: Methods a
Pindex Book 2018
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書目名稱AMPK影響因子(影響力)




書目名稱AMPK影響因子(影響力)學(xué)科排名




書目名稱AMPK網(wǎng)絡(luò)公開度




書目名稱AMPK網(wǎng)絡(luò)公開度學(xué)科排名




書目名稱AMPK被引頻次




書目名稱AMPK被引頻次學(xué)科排名




書目名稱AMPK年度引用




書目名稱AMPK年度引用學(xué)科排名




書目名稱AMPK讀者反饋




書目名稱AMPK讀者反饋學(xué)科排名




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https://doi.org/10.1007/978-981-99-7417-7 attaching UDP-glucose molecules linearly and α(1-6) linkages branching glucose chains every 8‐10 molecules to the main backbone chain. Glycogen synthase, branching enzyme, and glycogen phosphorylase are key enzymes involved in glycogen synthesis and degradation. These enzymes are tightly regulated
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Formation of the Contract of Employmentt to determine the extent and nature of these interactions for weakly formed protein-ligand complexes that have lifetimes of micro- to milliseconds. Nuclear magnetic resonance (NMR) spectroscopy is a powerful solution-based method for the atomic-level analysis of molecular interactions on a wide ran
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https://doi.org/10.1007/978-94-011-8836-4ver by traditional single molecule experimentation. System approaches that use a multi-tiered investigational methodology have been instrumental in advancing our understanding of cellular mechanisms that result in phenotypic changes. Here, we present a bioinformatics approach to identify AMP-activat
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Rights and Obligations of the Partiesifications, and isoform composition of the AMPK heterotrimer. It is becoming apparent that AMPK activation/inhibition by synthetic drugs involves similar levels of complexity. Major advances in our understanding of these mechanisms have been gained from recombinant expression systems that provide su
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