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Titlebook: YAC Protocols; David Markie Book 19961st edition Humana Press 1996

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書目名稱YAC Protocols
編輯David Markie
視頻videohttp://file.papertrans.cn/1061/1060179/1060179.mp4
叢書名稱Methods in Molecular Biology
描述Yeast artificial chromosomes (YACs) have their origins in the molecular genetic analysis of the yeast Saccharomyces cerevisiae. The construction of self-maintaining genetic elements from isolated frag- ments of the yeast genome defined DNA sequences necessary for chro- mosome function has provided telomeres, centromeres, and autonomous replicating sequences. In 1987 a reversal of the strategy put these short functional DNA sequences to work in cloning vectors, producing "yeast" chromosomes largely composed of foreign DNA. Initially the insert size of clones averaged several hundred kilobasepairs, a remarkable achieve- ment. Rapid progress with cloning technology has since enabled the construction of YAC libraries with average insert sizes of around 1 Mb, with many clones exceeding that size, and YACs remain the largest capacity microbiological cloning system available. They effectively bridge the size gap between bacterial cloning (plasmids, cosmids, PI, and bacterial artificial chromosomes) and what could be considered mammalian cloning systems (somatic cell hybrids and irradiati- fusion gene transfer hybrids). YACs also brought with them a conceptual revolution in the man- agemen
出版日期Book 19961st edition
版次1
doihttps://doi.org/10.1385/0896033139
isbn_ebook978-1-59259-541-9Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 1996
The information of publication is updating

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Methods in Molecular Biologyhttp://image.papertrans.cn/xyz/image/1060179.jpg
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1064-3745 alian cloning systems (somatic cell hybrids and irradiati- fusion gene transfer hybrids). YACs also brought with them a conceptual revolution in the man- agemen978-1-59259-541-9Series ISSN 1064-3745 Series E-ISSN 1940-6029
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Vertical Development of the Systems Approach and Cybernetics: Issues and Opportunities fundamental ideas are increasing. Such ideas include the idea of dynamic systems, the idea of purposeful behaviour, the idea of open systems; the idea of purposeful behaviour, the idea of self-organization, the idea of autopoiesis, and some others. The purpose of the report is to return the “vertic
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Friedrich-Wilhelm Schrader,Andreas Helmkeinclude such features as:.·???????? An extensive ?literature review, including coverage of research from fields other than marketing.·???????? an overview of how practitioners have addressed the topic.·???????? introduction of relevant analytical tools, such as statistics and ethnographic studies.·?
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