Titlebook: Wound Regeneration; Methods and Protocol Hiranmoy Das Book 2021 Springer Science+Business Media, LLC, part of Springer Nature 2021 Tissue r

organism

Cutaneous Wound Generation in Diabetic NOD/SCID Mice and the Use of Nanofiber-Expanded Hematopoietiblood, expand CD34. stem cells using a nanofiber scaffold (polyether sulfone-coated and amino group-treated), induce diabetes in immunocompromised (NOD/SCID) mice, induce a cutaneous wound in mice, and how to treat the wound with the nanofiber-expanded CD34. stem cells. This protocol also shows how to measure wound healing.

epicardium

Evaluation of MicroRNA Therapeutic Potential Using the Mouse In Vivo and Human Ex Vivo Wound Modelstial therapeutic targets for wound treatment. In this chapter, we describe a step-by-step protocol about how to evaluate the therapeutic potential of a miRNA in mouse in vivo and human ex vivo wound models. The findings from these preclinical wound models will serve as a basis for further clinical trials.

octogenarian

Generation of Acute Hind Limb Ischemia in NOD/SCID Mice and Treatment with Nanofiber-Expanded CD34+ellular and molecular mechanisms. This protocol describes the ischemic model development in NOD/SCID mice and the use of human umbilical cord blood-derived and nanofiber scaffold-expanded CD34. stem cells to investigate the efficacy of regenerative therapy.

HACK

CUMB

Corneal Repair Models in Mice: Epithelial/Mechanical Versus Stromal/Chemical Injuries,omal/chemical injury model. The two models induce different repair responses, and consequently enable the study of independent repair processes. Here, we describe how these two wound models may be used to study basic cellular and molecular mechanisms of corneal repair.

雪上輕舟飛過

Infection-Induced Porcine Ex Vivo Corneal Wound Model to Study the Efficacy of Herpes Simplex Virush, it is also a leading cause of infection-associated blindness. In this chapter, we discuss an in-depth protocol required to evaluate corneal damage due to HSV-1 infection using porcine models of ex vivo infection. Our methods can be adapted to study similar infections caused by other viruses and bacteria.

外面

A Murine Incisional Fetal Wound-Healing Model to Study Scarless and Fibrotic Skin Repair,ration and scarless healing in less developed skin or scarring and fibrotic healing in more developed skin. Here, a reproducible incisional wound model in developing mice is described that our lab has used repeatedly to study scarless and fibrotic fetal wound healing.

interior

圣人

就職

Investigating Epidermal Interactions Through an In Vivo Cutaneous Wound-Healing Assay,sely devise their experimental design. The coordination of immunological, epithelial, vascular, and microenvironmental factors in wound healing makes this technique a valuable tool for investigators across fields.