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Titlebook: Whole-Body Regeneration; Methods and Protocol Simon Blanchoud,Brigitte Galliot Book‘‘‘‘‘‘‘‘ 2022 The Editor(s) (if applicable) and The Auth

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發(fā)表于 2025-3-21 18:28:17 | 只看該作者 |倒序瀏覽 |閱讀模式
書目名稱Whole-Body Regeneration
副標題Methods and Protocol
編輯Simon Blanchoud,Brigitte Galliot
視頻videohttp://file.papertrans.cn/1029/1028082/1028082.mp4
概述This book is open access, which means that you have free and unlimited access.Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key n
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Whole-Body Regeneration; Methods and Protocol Simon Blanchoud,Brigitte Galliot Book‘‘‘‘‘‘‘‘ 2022 The Editor(s) (if applicable) and The Auth
描述.This Open Access volume provides a comprehensive overview of the latest tools available to scientists to study the many facets of whole-body regeneration (WBR). The chapters in this book are organized into six parts. Part One provides a historical overview on the study of the WBR phenomena focusing on the primary challenges of this research. Parts Two and Three explore a series of non-vertebrate zoological contexts that provide experimental models for WBR, showing how they can be approached with cellular tools. Parts Four, Five, and Six discuss the future advancements of WBR, reporting about the cutting-edge techniques in genetics and omics used to dissect the underlying mechanisms of WBR, and systems biology approaches to reach a synthetic view of WBR. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and thorough, .Whole-Body Regeneration: Methods and Protocols. is a valuable resource for scientists and researchers who
出版日期Book‘‘‘‘‘‘‘‘ 2022
關鍵詞immune response; cell sorting; stem cells; cell isolation; apoptosis; Open Access
版次1
doihttps://doi.org/10.1007/978-1-0716-2172-1
isbn_softcover978-1-0716-2174-5
isbn_ebook978-1-0716-2172-1Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s) 2022
The information of publication is updating

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Studying Hemichordata WBR Using , of a shallow beach with a coral reef. Here, we describe the methods for animal collection and preparation of regenerating tissues. The prepared tissues can be used for various molecular and/or histological experiments. We also demonstrate how to examine gene expression patterns in the tissues using whole mount in situ hybridization.
地板
發(fā)表于 2025-3-22 07:15:28 | 只看該作者
Studying Tunicata WBR Using a fully functional adult regenerates from a fragment of the vascular system in around 2?weeks after amputation. In this chapter, we present protocols to collect . colonies, confirm their species, breed them in the lab, monitor WBR and perform histological staining on cryosections.
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Studying Echinodermata Arm Explant Regeneration Using , tests, microscopic anatomy techniques (including transmission electron microscopy, TEM) used to analyze the regenerating explant tissues and cells plus a downstream RNA extraction protocol needed for subsequent molecular investigations.
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發(fā)表于 2025-3-23 01:31:30 | 只看該作者
The Hazards of Regeneration : From Morgan’s Legacy to Evo-Devomals and plants. Through his endeavor, he introduced a new way to study regeneration and its evolution, setting a conceptual framework that still guides today’s research and that embraces the contemporary evolutionary and developmental approaches..In the first part of the chapter, we summarize Morga
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發(fā)表于 2025-3-23 07:45:33 | 只看該作者
Studying Regeneration in Ascidians : An Historical Overviewespread in all the seas, constituting abundant communities in various ecosystems. Among chordates, only tunicates are able to reproduce asexually, forming colonies. The high regenerative potentialities enabling tunicates to regenerate damaged body parts, or the whole body, represent a peculiarity of
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