Titlebook: 3D Cell Culture; Methods and Protocol Zuzana Koledova Book 2017 Springer Science+Business Media LLC 2017 Hydrogels.3D Organoids.Scaffolds.O

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überbetriebliche Vernetzung im Handelive studies using genetic models of intestinal epithelium have revealed the mechanisms underlying the self-renewal of intestinal stem cells. Exploiting this knowledge, we developed a novel 3D culture system that enables the outgrowth of intestinal Lgr5. stem cells derived from mouse and human tissue

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Mittel und Methoden der Medizin, free floating collagen type I gels. In this assay, luminal cells predominantly form multicellular spheres, while basal/myoepithelial cells form complex branched structures resembling terminal ductal lobular units (TDLUs), the functional units of the human mammary gland in situ. The TDLU-like organo

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?kologische Kommunikation in Deutschlandl cells are seeded into 3D hydrogels with defined components, which include both proteins and carbohydrates present in mammary tissue. Over a span of 10–14 days, the seeded cells form mammary tissues with complex ductal-lobular topologies and include luminal and basal cells in the correct orientatio

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?kologische Kommunikation in Deutschlandechniques. Especially in prostate cancer research there is a big need for more tissue-recapitulating models to get a better understanding of the mechanisms driving prostate cancer as well as to screen for more efficient drugs that can be used for treatment. In this chapter we describe a 3D hanging d

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https://doi.org/10.1007/978-3-322-83299-3cell or cell–matrix interactions these models may be more predictive for the human situation compared to animal models or two-dimensional cell culture systems. Especially for dermatological research, skin models such as epidermal or full-thickness skin equivalents (FTSE) are used for different appli

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