| 期刊全稱 | 2D PAGE: Sample Preparation and Fractionation | | 期刊簡稱 | Volume 1 | | 影響因子2023 | Anton Posch | | 視頻video | http://file.papertrans.cn/101/100602/100602.mp4 | | 發(fā)行地址 | Contains step-by-step instructions that ensure successful results.Provides tricks of the trade and notes on troubleshooting and avoiding known pitfalls | | 學科分類 | Methods in Molecular Biology | | 圖書封面 |  | | 影響因子 | This book, split into two volumes, presents a broad coverage of the principles and recent developments of sample preparation and fractionation tools in Expression Proteomics in general and for two-dimensional electrophoresis (2-DE) in particular. 2-DE, with its unique capacity to resolve thousands of proteins in a single run, is still a fundamental research tool for nearly all protein-related scientific projects. The methods described here in detail are not limited to 2-DE and can also be applied to other protein separation techniques. Because each biological sample is unique, a suited sample preparation strategy has to consider the type of sample as well as the type of biological question being addressed. The complex nature of proteins often requires a multitude of sample preparation options. In addition, sample preparation is not only a prerequisite for a successful and reproducible Proteomics exp- iment, but also the key factor to meaningful data evaluation. Interestingly, not much attention was paid to this area during Proteomics methodology dev- opment and therefore this book is intended to explain in depth how proteins from various sources can be properly isolated and prepare | | Pindex | Book 2008 |
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