Titlebook: Homologous Recombination; Methods and Protocol Andrés Aguilera,Aura Carreira Book 2021 Springer Science+Business Media, LLC, part of Spring

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Holliday Junction Resolution,ranslational modifications like phosphorylation. Here, we describe a protocol to purify . Yen1 resolvase in two different phosphorylation states (high and low) and to set up a biochemical assay to compare their ability to process a synthetic, oligonucleotide-based Holliday junction structures.

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Genome-Wide Analysis of Mitotic Recombination in Budding Yeast, with limited experience in computational methods to conceptually understand and execute the steps of genome-wide LOH analysis as well as to adapt and apply them to their own specific studies and experimental models.

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Analysis of DNA Double-Strand Break End Resection and Single-Strand Annealing in ,dditional assay to follow resection involves recombination between two direct repeats by single-strand annealing (SSA), since SSA requires extensive resection to expose two single-strand repeats for annealing. The kinetics of resection and SSA repair are then measured using Southern blots.

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Biochemical Analysis of D-Loop Extension and DNA Strand Displacement Synthesis,s biochemical assays for (1) D-loop formation and DNA synthesis within the D-loop and (2) DNA strand displacement synthesis to test the role of DNA helicases (e.g., Pif1) in repair DNA synthesis. These mechanistic assays are valuable for elucidating the molecular details of HR.

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