Titlebook: Quantum Dots; Applications in Biol Marcel P. Bruchez,Charles Z. Hotz Book 2007 Humana Press 2007 FISH.Fluoreszenz in situ-Hybridisierung.Ge

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1064-3745 ve parts, the first two parts cover the use of QDs in imaging fixed and living cells (and tissues). Protocols are included for using QDs in routine (protein and structural cellular labeling), as well as enabling (single receptor trafficking, clinical pathology, correlative microscopy) applications.

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Immunofluorescent Labeling of Proteins in Cultured Cells With Quantum Dot Secondary Antibody Conjugaowever, QDs are structurally larger than organic dye probes and require different fixation and permeabilization conditions for optimum labeling. In the chapter, we describe several protocols for labeling proteins in nuclear, cytoplasmic, and membranous compartments with QD conjugates.

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