標題: Titlebook: Viroids; Methods and Protocol Ayala L. N. Rao,Irene Lavagi-Craddock,Georgios Vid Book 2022 Springer Science+Business Media, LLC, part of Sp [打印本頁] 作者: mountebank 時間: 2025-3-21 17:38
書目名稱Viroids影響因子(影響力)
書目名稱Viroids影響因子(影響力)學科排名
書目名稱Viroids網(wǎng)絡公開度
書目名稱Viroids網(wǎng)絡公開度學科排名
書目名稱Viroids被引頻次
書目名稱Viroids被引頻次學科排名
書目名稱Viroids年度引用
書目名稱Viroids年度引用學科排名
書目名稱Viroids讀者反饋
書目名稱Viroids讀者反饋學科排名
作者: 無所不知 時間: 2025-3-21 21:33
Oxana Kekstidou,Christina Varveri,Nikon Vassilakosndteile des Drei-S?ulen-Systems der Altersvorsorge bei abh?ngig Besch?ftigten m?glichst zu vermeiden, wird in diesem Abschnitt eine kurze systematische Darstellung der Kernpunkte des Einkommensteuergesetzes in Zusammenhang mit der Altersvorsorge gegeben.作者: linguistics 時間: 2025-3-22 00:28 作者: STRIA 時間: 2025-3-22 08:17 作者: 發(fā)源 時間: 2025-3-22 10:57 作者: 釋放 時間: 2025-3-22 13:47 作者: theta-waves 時間: 2025-3-22 19:18
Detection of Viroids Using RT-qPCRptase generates a cDNA copy of a portion of the target RNA molecule. After first-strand cDNA synthesis, RNA template from cDNA:RNA hybrid molecule is removed by digestion with RNase H to improve the sensitivity of PCR step. This cDNA is then be used as a template for amplification of viroid sequence in PCR.作者: Aspirin 時間: 2025-3-22 23:41 作者: dysphagia 時間: 2025-3-23 03:03 作者: Chivalrous 時間: 2025-3-23 07:01
Subhas Hajeri,James Ng,Jude Grosser,Georgios Vidalakis Dienstleistungsunternehmen l?ngst . (für die Schule vgl. u.a. Hausser 1981, Schittko 1984) — in einer prim?r funktional differenzierten Gesellschaft kaum Informationswert besitze. Der Altenpflege jedenfalls war dieses Qualit?tsma? bisher nicht gel?ufig.作者: crescendo 時間: 2025-3-23 11:29
Study and Detection of Citrus Viroids in Woody Hosts citrus variants of the hop stunt viroid (i.e., CVd-IIb and CVd-IIc), the clonal indicator ‘Parson’s special # 9’ mandarin (. Blanco) is preferred. Inoculation techniques and symptom expression are described in detail. Other supporting elements, such as greenhouse conditions and propagation techniques, are also presented.作者: CUB 時間: 2025-3-23 13:53
Analysis on RNA Motif-Based RNA Trafficking in Plantse the methods for generating mutational variants using site-directed mutagenesis and infection assays to unravel the function of RNA motifs. This approach can be modified to study other RNA motif-based biological processes.作者: 柔軟 時間: 2025-3-23 20:18
High-Throughput RNA Extraction from Citrus Tissues for the Detection of Viroidsf high-quality uniform total nucleic acids for each individual sample. Here, we describe a streamlined and optimized protocol for citrus tissue processing and RNA extraction that can be used for downstream applications such as viroid detection by reverse transcription-quantitative polymerase chain reaction.作者: GULF 時間: 2025-3-23 23:01 作者: 揉雜 時間: 2025-3-24 04:14 作者: Vo2-Max 時間: 2025-3-24 10:21
Gel Blot Hybridization for Viroids electrophoresis under denaturing conditions, hybridized with a viroid full-length riboprobe labeled with chemiluminescence. Viroid detection with this approach entails positive signals, specific migration, and the differentiation of their circular and linear forms.作者: 熱心助人 時間: 2025-3-24 12:43
Tissue Print Hybridization for Detection and Characterization of Viroidsly implemented as long as factors like stringency and low viroid titer are properly adjusted to enable enhancement of the detection limit. The protocol described allows for indexing hundreds of field samples as a phytosanitary control measure.作者: 異教徒 時間: 2025-3-24 16:29 作者: 昆蟲 時間: 2025-3-24 22:28 作者: osteopath 時間: 2025-3-25 01:39 作者: 織物 時間: 2025-3-25 03:47 作者: temperate 時間: 2025-3-25 08:09
High-Throughput RNA Extraction from Citrus Tissues for the Detection of Viroidsrveys, and other regulatory, quarantine, or research diagnostic programs are increasingly dependent on high-throughput tissue pulverization and nucleic acid extraction protocols. Magnetic bead-based approaches using semi-automated robotic equipment allow high-throughput extraction and purification o作者: entail 時間: 2025-3-25 12:18
Extraction and Purification of Viroids from Herbaceous Hostsaditional protocols that require large amounts of starting tissue and take several days to perform to those based on column chromatography which is more efficient and can be performed with smaller amounts of infected tissue. The goal of all protocols is to enrich for RNA fractions that contain viroi作者: 外星人 時間: 2025-3-25 18:16 作者: critic 時間: 2025-3-25 22:31
Two-Dimensional Polyacrylamide Gel Electrophoresis Analysis of Viroid RNAsy in mobility that circular RNAs experience under denaturing conditions. First PAGE separates RNA preparations from viroid-infected plants, and the whole lane from this first gel is next perpendicularly loaded on top of a second gel. Separation continues under new conditions that differ in the degre作者: 歌劇等 時間: 2025-3-26 02:23
A Simplified Dot-Blot Hybridization Protocol for , Detection in Solanaceaebe and a simplified extraction procedure that avoids the use of hazardous chemicals. PSTVd was detected in composite tomato leaf samples in a ratio of up to 1:15 of PSTVd-infected to non-infected tissue and in composite potato tuber samples in a ratio up to 1:5 of PSTVd-infected to non-infected tiss作者: FATAL 時間: 2025-3-26 07:03
Gel Blot Hybridization for Viroidsloped about viroids and also an excellent method for viroid detection. Here we describe the methodology of a Northern blot based in polyacrylamide gel electrophoresis under denaturing conditions, hybridized with a viroid full-length riboprobe labeled with chemiluminescence. Viroid detection with thi作者: Ascribe 時間: 2025-3-26 12:33
Tissue Print Hybridization for Detection and Characterization of Viroidson technique, such as tissue printing. After fixing the samples on the membrane, hybridization steps similar to Northern or dot blot can be successfully implemented as long as factors like stringency and low viroid titer are properly adjusted to enable enhancement of the detection limit. The protoco作者: ALTER 時間: 2025-3-26 16:13 作者: osteocytes 時間: 2025-3-26 17:04 作者: Canvas 時間: 2025-3-27 00:59
Detection of Viroids Using RT-qPCRal diseases in economically important crops. With the advent of thermal cyclers incorporating fluorescent detection, reverse transcription coupled to the quantitative polymerase chain reaction (RT-qPCR) has transformed the way the viroids are detected. The method involves using sequence-specific pri作者: debble 時間: 2025-3-27 01:30 作者: Endemic 時間: 2025-3-27 08:44
Real-Time Detection of Viroids Using Singleplex and Multiplex Quantitative Polymerase Chain Reactionnt reporter dyes with distinct fluorescent spectra. The number of reporter fluorophores is typically restricted to three or four, depending upon the capability of the real-time PCR platform and software used. Each target is amplified by a different set of primers and a uniquely labeled probe that di作者: 香料 時間: 2025-3-27 11:15 作者: evasive 時間: 2025-3-27 14:08
Tyler Dang,Fatima Osman,Jinbo Wang,Tavia Rucker,Sohrab Bodaghi,Shih-hua Tan,Deborah Pagliaccia,Irenell. Kritiker werden nun anmerken, da? das Alter an sich der wichtigste kardiovaskul?re Risikofaktor ist und da? sich auch bei ?lteren Patienten mit normalem Blutdruck die Inzidenz von kardiovaskul?ren Ereignissen deutlich erh?ht.作者: configuration 時間: 2025-3-27 18:57 作者: Panther 時間: 2025-3-27 23:00 作者: Trypsin 時間: 2025-3-28 04:42
ties of negative polarity items (NPIs) on the one hand and of negative-words (N-words) on the other. . exhibits sensitivity to the entailment patterns associated with the sentence in which it occurs, a feature that characterizes NPIs in general. Moreover, . gives rise to different meanings depending作者: CON 時間: 2025-3-28 08:36 作者: 裁決 時間: 2025-3-28 13:21
Robert R. Krueger,Georgios Vidalakisilities and may involve feeding millions of people. This potential for affecting the lives and well-being of many people requires that food processors maintain a moral obligation to provide wholesome and safe food for consumers. There are laws and regulations that provide guidance for proper practic作者: Control-Group 時間: 2025-3-28 15:02
Heather N. Smith,Junfei Ma,Ying Wangh we have argued are an incidental part of FDI. Instead it considers FDI as a consequence of the internalisation of markets for proprietary information and other intermediate products. The costs and benefits we consider are those of an internal market, in comparison to an external market.作者: Cpap155 時間: 2025-3-28 22:09 作者: FRAUD 時間: 2025-3-29 02:11
erschiedene Altersgrenzen. Sie sind vom 60. bis zum 67. Lebensjahr abgestuft und bilden in ihrer Gesamtheit das System der flexiblen Altersgrenze. Die wichtigste ?nderung des vierten Rentenversicherungs?nderungsgesetzes war die gesetzliche Festlegung der formalen Wahlfreiheit für M?nner, den Beginn 作者: Mercurial 時間: 2025-3-29 06:11
Tyler Dang,Fatima Osman,Jinbo Wang,Tavia Rucker,Sohrab Bodaghi,Shih-hua Tan,Deborah Pagliaccia,Irenepertonie die kardiovaskul?re Prognose verbessert [1–3], bestand doch lange Zeit die Meinung, da? eine Hypertonie im Alter ein physiologischer Anpassungsmechanismus an ver?nderte kardiale und vaskul?re Bedingungen sein k?nnte. Das kardiovaskul?re Risiko beim ?lteren Hypertoniker ist im Vergleich zu j作者: 煉油廠 時間: 2025-3-29 07:20
Rosemarie W. Hammondich hier um eine relativ neue Forschungsrichtung. Dafür, da? sie sich erst in den letzten Jahrzehnten entwickelt hat, k?nnen verschiedene Gründe angeführt werden. Einmal ist die ganze Alterspsychiatrie — wie die übrigen Kapitel dieses Bandes zeigen — eine junge Wissenschaft. Im letzten Jahrhundert w作者: aristocracy 時間: 2025-3-29 11:53
José-Antonio Daròs psychologischen Ver?nderungen pr?gen sehr oft das Bild des ?lteren Menschen vor allem in seinen letzten Jahren wesentlich deutlicher als die rein somatischen Ver?nderungen. Die psychisch-intellektuelle Situation des ?lteren Menschen wird dabei nicht nur durch die somatische Involution speziell des 作者: APEX 時間: 2025-3-29 15:56
Oxana Kekstidou,Christina Varveri,Nikon Vassilakosrbst?tigen und auch zwischen verschiedenen Vorsorgema?nahmen Parallelen aufweist. Um daher überschneidungen in der nachfolgenden Darstellung der Bestandteile des Drei-S?ulen-Systems der Altersvorsorge bei abh?ngig Besch?ftigten m?glichst zu vermeiden, wird in diesem Abschnitt eine kurze systematisch作者: 起來了 時間: 2025-3-29 23:24 作者: 缺乏 時間: 2025-3-30 02:17 作者: 公共汽車 時間: 2025-3-30 04:14
Shachinthaka D. Dissanayaka Mudiyanselage,Ying Wang sind. Der Verlag stellt mit diesem Archiv Quellen für die historische wie auch die disziplingeschichtliche Forschung zur Verfügung, die jeweils im historischen Kontext betrachtet werden müssen. Dieser Titel erschien in der Zeit vor 1945 und wird daher in seiner zeittypischen politisch-ideologischen作者: 貧窮地活 時間: 2025-3-30 10:05
Nerida J. Donovan,Grant A. Chambers,Mengji Cao difficult question: where does one begin? Every beginning requires an assumption, and yet the idea of a beginning is that it does not include an assumption, that it is prior to any assumption. One has to ask, where does and how does one begin from? This question gets a further complicated twist whe作者: malign 時間: 2025-3-30 15:28 作者: 完成 時間: 2025-3-30 20:06
Methods in Molecular Biologyhttp://image.papertrans.cn/v/image/983282.jpg作者: affluent 時間: 2025-3-31 00:27
https://doi.org/10.1007/978-1-0716-1464-8sPAGE gel electrophoresis; Polyprobes; RT-PCR; Multiplex RT-PCR; Next-generation sequencing作者: enflame 時間: 2025-3-31 02:32 作者: mighty 時間: 2025-3-31 05:51
Heather N. Smith,Junfei Ma,Ying Wangh we have argued are an incidental part of FDI. Instead it considers FDI as a consequence of the internalisation of markets for proprietary information and other intermediate products. The costs and benefits we consider are those of an internal market, in comparison to an external market.作者: gentle 時間: 2025-3-31 11:54
Shachinthaka D. Dissanayaka Mudiyanselage,Ying Wang sind. Der Verlag stellt mit diesem Archiv Quellen für die historische wie auch die disziplingeschichtliche Forschung zur Verfügung, die jeweils im historischen Kontext betrachtet werden müssen. Dieser Titel erschien in der Zeit vor 1945 und wird daher in seiner zeittypischen politisch-ideologischen Ausrichtung vom Verlag nicht beworben.作者: 橢圓 時間: 2025-3-31 16:02 作者: Verify 時間: 2025-3-31 19:45 作者: Dissonance 時間: 2025-4-1 00:35 作者: aspersion 時間: 2025-4-1 02:09 作者: 極為憤怒 時間: 2025-4-1 10:04
