標(biāo)題: Titlebook: Viral Vectors for Gene Therapy; Methods and Protocol Fredric P. Manfredsson,Matthew J. Benskey Book 2019 Springer Science+Business Media, L [打印本頁] 作者: 里程表 時間: 2025-3-21 17:48
書目名稱Viral Vectors for Gene Therapy影響因子(影響力)
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書目名稱Viral Vectors for Gene Therapy網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Viral Vectors for Gene Therapy被引頻次
書目名稱Viral Vectors for Gene Therapy被引頻次學(xué)科排名
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書目名稱Viral Vectors for Gene Therapy年度引用學(xué)科排名
書目名稱Viral Vectors for Gene Therapy讀者反饋
書目名稱Viral Vectors for Gene Therapy讀者反饋學(xué)科排名
作者: CHECK 時間: 2025-3-21 20:37 作者: Diaphragm 時間: 2025-3-22 00:32 作者: 刀鋒 時間: 2025-3-22 05:14
Ivette M. Sandoval,Nathan M. Kuhn,Fredric P. Manfredsson作者: hypnotic 時間: 2025-3-22 10:26
Stuart Tinch,Kathy Szczur,William Swaney,Lilith Reeves,Scott R. Witting作者: Mangle 時間: 2025-3-22 15:39
Michael T. Massengill,Brianna M. Young,Alfred S. Lewin,Cristhian J. Ildefonso作者: 懸掛 時間: 2025-3-22 18:56
Book 2019on troubleshooting and avoiding known pitfalls..Comprehensive and authoritative, .Viral Vectors for Gene Therapy: Methods and Protocols. is a valuable resource for researchers, clinicians, and students looking to utilize viral vectors in gene therapy experiments..作者: arthrodesis 時間: 2025-3-23 01:05
Basic Concepts in Viral Vector-Mediated Gene Therapy potentially prohibitive, especially to the novice gene therapist. In order to aid in this challenging process, here we provide an overview of basic gene therapy modalities and a decision tree that can be used to make oneself aware of the options available to the beginning gene therapist. This infor作者: 增強(qiáng) 時間: 2025-3-23 03:06 作者: Statins 時間: 2025-3-23 09:33
A Scalable Lentiviral Vector Production and Purification Method Using Mustang Q Chromatography and Tcted between 24 and 48?h post-transfection. The supernatant may then be purified by Mustang Q chromatography, concentrated by Tangential Flow Filtration, and finally diafiltered into the final formulation buffer of choice. Here we describe a pilot scale method for the manufacture of a Lentiviral vec作者: Valves 時間: 2025-3-23 13:41
Poxviruses as Gene Therapy Vectors: Generating Poxviral Vectors Expressing Therapeutic Transgeneshe demands of different treatment needs, VACV and MYXV can be genetically engineered to express therapeutic transgenes. The engineering process used in poxvirus vectors can be very different from that of other DNA virus vectors (e.g., the herpesviruses). This chapter is intended to serve as a guide 作者: intoxicate 時間: 2025-3-23 16:03
AAV-Mediated Gene Delivery to the Mouse Liverical manuscript focuses on AAV-mediated transduction of the mouse liver. Xenograft models, in which chimeric mouse-human livers can be established, are also amenable to AAV-mediated gene transfer and have proven to be powerful tools for in vivo selection and characterization of novel human-specific 作者: Stress-Fracture 時間: 2025-3-23 20:59 作者: 泰然自若 時間: 2025-3-23 23:12 作者: ineluctable 時間: 2025-3-24 05:45 作者: pulmonary 時間: 2025-3-24 08:11 作者: 休戰(zhàn) 時間: 2025-3-24 13:48 作者: Brain-Waves 時間: 2025-3-24 18:04
elche eine überstellte B?schung erfordern, werden im B?schungsbereich Gesteine in eine ver?nderte Position und Lage zur angreifenden Witterung gebracht. Durch den Erdaushub herrscht für die in der B?schung verbleibenden Gesteine ein natürliches Ungleichgewicht. Unter geringerer Gesteinsauflast und v作者: Lymphocyte 時間: 2025-3-24 19:35
Sharon C. Cunningham,Ian E. Alexanderung der Vortriebsleistungen bis zu 100%. Mit Hilfe des Schmidt-Prallhammers kann der Einflu?bereich einer St?rungsfl?che im Gebirge nachgewiesen werden, was Rückschlüsse erlaubt auf die Beeinflussung des mechanischen Vortriebs..In Gesteinen mit gneisiger bis schiefriger Textur spielt die Richtung de作者: hurricane 時間: 2025-3-24 23:48
C. J. Rocca,S. Cherquiller- dings inzwischen dazu beigetragen, da? verst?rkt auch über vertikale Strategien innerhalb der Unternehmung, zwischen Unternehmungen und ihren Zulieferern, aber auch Unternehmungen und ihren Wettbewerbern diskutiert wird. Dabei sind speziell Fragen in der betriebswirtschaftlichen Literatur von 作者: 詞匯 時間: 2025-3-25 05:37
tionsweise javanischer D?rfer analysiert. Somit standen vor allem Patron-Klient-Beziehungen und eine auf dem Besitz von Reisfeldern basierende soziale Schichtung im Mittelpunkt der Fallstudien. Diese Elemente aber spielen in den D?rfern der Hochlandregionen von Gunung Kidul nur eine marginale Rolle.作者: jocular 時間: 2025-3-25 11:02 作者: hurricane 時間: 2025-3-25 11:43 作者: 畏縮 時間: 2025-3-25 19:54
Ekramy E. Sayedahmed,Rashmi Kumari,Suresh K. Mittal Formen der Rückwendung und Vorausdeutung macht der Autor übergreifende Zusammenh?nge eigens kenntlich. Schon haben sich aber gelegentlich Ausblicke auf weitere Symptome der Vorgangsverflechtung ergeben. Die Besch?ftigung mit Begleitmotiven, Allegorien, Symbolen und schlie?lich mit dem Wechselspiel 作者: Overstate 時間: 2025-3-25 20:14
Andranik Kahramanian,Toshihiko Kuroda,Hiroaki Wakimotodere bezüglich der Praxis der Massnahmen. Sie setzt die Arbeit der Inventarisation durch vertiefende Forschung am Einzelfall. fort. Die Kl?rung der Baugeschichte ist notwendige Voraussetzung für die Erarbeitung eines denkmalpflegerischen Konzepts, wenn die Massnahme in die Substanz des Denkmals eing作者: anesthesia 時間: 2025-3-26 01:17 作者: 華而不實(shí) 時間: 2025-3-26 07:22 作者: JADED 時間: 2025-3-26 09:20 作者: 整體 時間: 2025-3-26 12:50
C. J. Rocca,S. Cherquie st?rke- re Au?enorientierung im Sinne einer marktorientierten Unternehmensplanung zu- nehmende Bedeutung erfahren. Die Planungskonzepte setzen dabei vornehmlich an der strategischen Gesch?ftsfeldplanung an und versuchen, zur Gestaltung des Gesch?ftsfeldportfolios beizutragen. Die Problematik diese作者: 不可接觸 時間: 2025-3-26 18:01 作者: Pillory 時間: 2025-3-26 21:19
Design and Assembly of CRISPR/Cas9 Lentiviral and rAAV Vectors for Targeted Genome Editingogenous gene expression and labeling of genomic loci, among other applications. This chapter provides an introduction to the basis of the technology and a detail protocol for the most classic application: gene inactivation by CRISPR/Cas9 nuclease system from . This workflow can be easily adapted for other CRISPR systems and applications.作者: MIME 時間: 2025-3-27 01:21
Design, Construction, and Application of Transcription Activation-Like Effectorseffector domains that alter the function of the TALE upon binding. This flexibility of TALE design and downstream effect may offer therapeutic applications that are discussed in this section. Finally, we provide a future perspective on TALE technology and what challenges remain for successful translation of gene-editing strategies to the clinic.作者: Immunotherapy 時間: 2025-3-27 09:06
Current Use of Adenovirus Vectors and Their Production Methodsasy to manipulate, have broad tropism, and have the capability to yield high titers, this delivery system has a wide range of applications for different clinical settings. This chapter emphasizes on some of the current usages of AdV vectors and their production methods.作者: 柔軟 時間: 2025-3-27 11:21 作者: 決定性 時間: 2025-3-27 15:17
Surgical Methods for Inner Ear Gene Delivery in Neonatal Mouseery in neonatal mouse inner ear: the round window approach and the posterior semicircular canal approach. Both of these approaches are effective at delivering gene therapy to the neonatal mouse inner ear.作者: 不規(guī)則 時間: 2025-3-27 18:23 作者: 滲入 時間: 2025-3-28 01:15
Multimodal Production of Adeno-Associated Virusylation). Moreover, we also describe the protocol for anion exchange chromatography, which can be used after the iodixanol gradient as an additional purification step. Last, we provide various protocols for determining virus titer.作者: 聽覺 時間: 2025-3-28 02:47
Generation of High-Titer Pseudotyped Lentiviral Vectorsing identified for special applications of LVs. Here we describe the methodology to generate pseudotyped LVs using a four-plasmid transient transfection system focusing on aspects to generate high-titer vector stocks.作者: PRE 時間: 2025-3-28 08:04 作者: Calculus 時間: 2025-3-28 13:36 作者: HIKE 時間: 2025-3-28 15:25
Design, Construction, and Application of Transcription Activation-Like Effectorsquences following a simple cipher. Customized TALE proteins can be used in a variety of molecular applications that include gene editing and transcriptional modulation. Presently, we provide a brief primer on the design and construction of TALEs. TALE proteins can be fused to a variety of different 作者: 內(nèi)閣 時間: 2025-3-28 20:15 作者: Visual-Field 時間: 2025-3-29 01:29
AAV Production Using Baculovirus Expression Vector Systemic or cancer diseases. The most efficient gene transfer tools are currently derived from viruses. Among them, the recombinant adeno-associated viruses (AAVs) are vectors of choice for many fundamental and therapeutic applications. The increasing number of clinical trials involving AAVs demonstrates 作者: foliage 時間: 2025-3-29 05:49
Multimodal Production of Adeno-Associated Viruscelerated pace. Nevertheless, despite its popularity, AAV is a relatively cumbersome virus to produce; however, significant efforts have been invested to develop, optimize, and simplify methodology that allows the generation of high-quality AAV with significantly increased production yields. Here we作者: 察覺 時間: 2025-3-29 11:09 作者: floaters 時間: 2025-3-29 15:15
A Scalable Lentiviral Vector Production and Purification Method Using Mustang Q Chromatography and Ttions in the genome. This status can be attributed primarily to their ability to transduce dividing as well as quiescent cells. When coupled with internal promotor selection to drive expression in one cell type but not another, the ease with which the vectors can be pseudotyped to either restrict or作者: Injunction 時間: 2025-3-29 19:13 作者: G-spot 時間: 2025-3-29 23:16
Construction of Oncolytic Herpes Simplex Virus with Therapeutic Genes of InterestC) has been accelerating translational research of oncolytic HSV (oHSV) as a promising therapeutic for refractory cancers such as glioblastoma, the deadliest primary malignancy in the brain. The large genome size of HSV readily allows arming of oHSV by incorporating therapeutic transgenes within the作者: Insensate 時間: 2025-3-30 02:01
Poxviruses as Gene Therapy Vectors: Generating Poxviral Vectors Expressing Therapeutic Transgenes and for vaccine development. More importantly, the oncolytic properties of poxviruses have led to their development as cancer therapeutics. Two poxviruses, vaccinia virus (VACV) and myxoma virus (MYXV), have been extensively studied as virotherapeutics with promising results. Vaccinia virus vectors作者: 脫落 時間: 2025-3-30 07:31
AAV-Mediated Gene Delivery to the Mouse Liverrently the most popular gene delivery system for targeting the liver, reflecting high transduction efficiency in vivo and the availability of a toolkit of multiple different capsids with high liver tropism. While AAV vectors confer stable gene transfer in the relatively quiescent adult liver, the pr作者: 集合 時間: 2025-3-30 08:56
