標(biāo)題: Titlebook: Vaccinia Virus and Poxvirology; Methods and Protocol Stuart N. Isaacs Book 20041st edition Humana Press 2004 [打印本頁(yè)] 作者: 萬(wàn)圣節(jié) 時(shí)間: 2025-3-21 18:42
書(shū)目名稱Vaccinia Virus and Poxvirology影響因子(影響力)
書(shū)目名稱Vaccinia Virus and Poxvirology影響因子(影響力)學(xué)科排名
書(shū)目名稱Vaccinia Virus and Poxvirology網(wǎng)絡(luò)公開(kāi)度
書(shū)目名稱Vaccinia Virus and Poxvirology網(wǎng)絡(luò)公開(kāi)度學(xué)科排名
書(shū)目名稱Vaccinia Virus and Poxvirology被引頻次
書(shū)目名稱Vaccinia Virus and Poxvirology被引頻次學(xué)科排名
書(shū)目名稱Vaccinia Virus and Poxvirology年度引用
書(shū)目名稱Vaccinia Virus and Poxvirology年度引用學(xué)科排名
書(shū)目名稱Vaccinia Virus and Poxvirology讀者反饋
書(shū)目名稱Vaccinia Virus and Poxvirology讀者反饋學(xué)科排名
作者: Barrister 時(shí)間: 2025-3-21 23:44
Construction of cDNA Libraries in Vaccinia Virus,st commonly used method to construct recombinant poxvirus is homologous recombination. The frequency of recombinants derived in this manner is of the order of 0.1%, sufficient to recover a recombinant of a purified DNA clone in a transfer plasmid, but far too low to permit construction of a represen作者: Cupping 時(shí)間: 2025-3-22 02:38 作者: 高度 時(shí)間: 2025-3-22 06:25
Monitoring of Human Immunological Responses to Vaccinia Virus,imple enzyme-linked immunosorbent assay (ELISA) to quantify vaccinia virus antibody titer. Additionally, to define serum-neutralizing activity, both a classical plaque reduction assay and a high-throughput 96-well plate method based on reduction of recombinant vaccinia virus expressed β-galactosidas作者: 禁止 時(shí)間: 2025-3-22 10:55
Vaccinia Virus as a Tool for Immunologic Studies,C class II-restricted antigen processing and presentation owing to many reasons specified in this chapter. This chapter aims to describe the commonly used procedures in this field that employ vaccinia virus systems, particularly troubleshooting common problems encountered during experiments.作者: 詞匯 時(shí)間: 2025-3-22 16:45 作者: 生來(lái) 時(shí)間: 2025-3-22 18:45 作者: 具體 時(shí)間: 2025-3-22 23:05 作者: 大氣層 時(shí)間: 2025-3-23 05:25 作者: mighty 時(shí)間: 2025-3-23 07:52 作者: 連詞 時(shí)間: 2025-3-23 09:47 作者: 凝視 時(shí)間: 2025-3-23 14:07 作者: seruting 時(shí)間: 2025-3-23 18:42 作者: 他去就結(jié)束 時(shí)間: 2025-3-24 02:12
Cynthia F. Wrightthe thickness of the Mesozoic sedimentary fill (more than 10 km) of the Arzacq basin are detailed. Northward-dip ping reflectors (at 2–3.5 s) beneath the flysch basin of Mauléon are also detected but are still difficult to interpret (basement or Jurassic?).作者: grudging 時(shí)間: 2025-3-24 05:48
Paul D. Gershonds solving the many problems caused by these substances. The papers in this volume are divided into five categories: two plenary session addresses given by J.P. Bruce and O. Hutzinger, 11 papers describing quantitative analytical results, 12 papers on analytical methods, 9 papers on incidence, monit作者: Adj異類的 時(shí)間: 2025-3-24 06:44
Mansun Law,Geoffrey L. Smith. The editors take great pleasure in expressing their thanks to the contributors for their participation and cooperation, and to Springer Verlag for personal and technical assistance in publishing the proceedings. We sincerely hope that this volume will contribute to improving the treat- ment for hy作者: Dysarthria 時(shí)間: 2025-3-24 12:25 作者: SOB 時(shí)間: 2025-3-24 15:10
Bruce T. Seet,Grant McFadden as giving detailed explanations of where, when and how gums are incorporated into products in these industries. The text is also accom- panied by a detailed index, designed to help the reader locate information easily. I wish to thank the publishers for giving me the opportunity to write this book. Their pat978-1-4613-7933-1978-1-4615-6385-3作者: 原始 時(shí)間: 2025-3-24 20:03 作者: HARP 時(shí)間: 2025-3-25 03:03 作者: 尖牙 時(shí)間: 2025-3-25 04:52 作者: LAPSE 時(shí)間: 2025-3-25 07:33 作者: heterodox 時(shí)間: 2025-3-25 13:05 作者: 聚集 時(shí)間: 2025-3-25 17:43 作者: 排斥 時(shí)間: 2025-3-25 22:45 作者: 詳細(xì)目錄 時(shí)間: 2025-3-26 00:46 作者: 使堅(jiān)硬 時(shí)間: 2025-3-26 04:58
Paul D. Gershonin the Aquatic Environment, May 23-25, 1978. The Symposium was organized by the National Water Research Institute of Environment Canada and the Institute for Environmental Studies of the University of Toronto. The purpose of the Symposium and of this volume was to bring together information on the a作者: nostrum 時(shí)間: 2025-3-26 08:58
Mansun Law,Geoffrey L. Smiththe basic understanding, diagnosis, and treatment of hydrocephalus. The present volume contains the proceedings of the International Symposium on "Hydrocephalus" that was held in Kobe, Japan, from November 12-14, 1990. The purpose of the symposium was to highlight the recent advances in this field a作者: 不能妥協(xié) 時(shí)間: 2025-3-26 16:06
Brian M. Ward freshwater plants and animals. The result of this interaction can be considered as the freshwater hydro climate and this plays the same role as that of conventional climate in terrestrial ecology. Agriculture, for example, has long been supported by specialist meteorological services which not only作者: MENT 時(shí)間: 2025-3-26 20:21 作者: Lignans 時(shí)間: 2025-3-26 22:48 作者: infringe 時(shí)間: 2025-3-27 05:08 作者: Gentry 時(shí)間: 2025-3-27 07:41
Transient and Inducible Expression of Vaccinia/T7 Recombinant Viruses,lating T7 RNA polymerase expression, and integration of .I under vaccinia promoter control into the viral genome, vT7lacOI, yielded a recombinant virus capable of IPTG-inducible T7 promoter-controlled expression of foreign genes.作者: VEIL 時(shí)間: 2025-3-27 09:32
Working Safely with Vaccinia Virus,roblems in normal hosts. This chapter reviews standard operating procedures when working with vaccinia virus and issues surrounding the use of prophylactic smallpox vaccination for laboratory workers.作者: Mutter 時(shí)間: 2025-3-27 14:58
Construction and Isolation of Recombinant Vaccinia Virus Using Genetic Markers,detail two different selection procedures: one relies on plaque formation phenotype using the vaccinia virus gene F13L; the other relies on antibiotic resistance using the . xanthine-guanine phosphoribosyl transferase gene.作者: Focus-Words 時(shí)間: 2025-3-27 20:28 作者: ventilate 時(shí)間: 2025-3-27 22:51
Orthopoxvirus Diagnostics,h three different methods that should be suitable for the preparation of orthopoxvirus DNA from various sources (e.g., clinical specimens or cell cultures) and four different methods for PCR that should be useful for investigating orthopoxvirus species and strains.作者: concentrate 時(shí)間: 2025-3-28 04:13 作者: 蝕刻 時(shí)間: 2025-3-28 08:58
Interaction Analysis of Viral Cytokine-Binding Proteins Using Surface Plasmon Resonance,s basic procedures for employing SPR to study the interaction between poxvirus proteins and host immune-signaling proteins. We also identify how this methodology may be adapted toward other applications relevant in the study of poxvirus-host interactions.作者: misanthrope 時(shí)間: 2025-3-28 10:36
Working Safely with Vaccinia Virus,interactions, and as a protein expression system and a vaccine vector. The ubiquitous use of vaccinia viruses in the laboratory raises certain safety concerns, because the virus can be a pathogen in individuals with immunological and dermatological abnormalities and, on occasion, can cause serious p作者: curettage 時(shí)間: 2025-3-28 15:54 作者: Mammal 時(shí)間: 2025-3-28 20:27 作者: 送秋波 時(shí)間: 2025-3-29 00:26 作者: 情感脆弱 時(shí)間: 2025-3-29 06:48
Construction of Recombinant Vaccinia Viruses Using Leporipoxvirus-Catalyzed Recombination and Reactfectious virus can be produced if purified poxvirus DNA is transfected into cells previously infected with another poxvirus. This process is termed . if the infecting virus is different from the transfected virus. We describe a method in which the high-frequency recombination and replication reactio作者: 男生戴手銬 時(shí)間: 2025-3-29 08:03 作者: conjunctivitis 時(shí)間: 2025-3-29 13:25 作者: 鋪?zhàn)?nbsp; 時(shí)間: 2025-3-29 18:22
Growing Poxviruses and Determining Virus Titer,gs. The main principle of isolating the virus is to mechanically lyse infected cells. The virus can then be semipurified by centrifugation through a sucrose cushion. Further purification can be achieved with a sucrose gradient. This chapter describes methods for the preparation of large-scale growth作者: Rodent 時(shí)間: 2025-3-29 20:00 作者: ULCER 時(shí)間: 2025-3-30 00:20
Orthopoxvirus Diagnostics, amplification, with either restriction cleavage or sequencing of amplicons, has been gaining credibility as a more rapid, specific, sensitive, and often cost-saving technique for research and diagnostic laboratories. This chapter is consolidated using prior research papers from our laboratories wit作者: Chagrin 時(shí)間: 2025-3-30 05:05 作者: babble 時(shí)間: 2025-3-30 10:11
Methods for Analysis of Poxvirus DNA Replication,eral of these proteins have been identified through genetic and biochemical evaluation, including the catalytic DNA polymerase (E9), an essential and stoichiometric component of the processive polymerase (A20), a singlestrand DNA-binding protein (I3), a type I topoisomerase (H6), the uracil DNA glyc作者: 失望昨天 時(shí)間: 2025-3-30 13:47
Studying the Binding and Entry of the Intracellular and Extracellular Enveloped Forms of Vaccinia V (IMV) and extracellular enveloped virus (EEV)—using immunofluorescent staining and confocal microscopy. After binding to or penetration of the cells, IMV, EEV, and virus cores are distinguished by different antibodies. Bound virus or penetrated cores are visualized and recorded by confocal microsco作者: Gene408 時(shí)間: 2025-3-30 16:38
Pox, Dyes, and Videotape,s a viral envelope-specific, green fluorescent protein (GFP) chimera produce enveloped virions that fluoresce green. This fluorescent labeling allows the live, real-time study of viral egress using a variety of microscopic techniques. The methods presented here describe how to image the movement of 作者: exigent 時(shí)間: 2025-3-30 23:42
Interaction Analysis of Viral Cytokine-Binding Proteins Using Surface Plasmon Resonance,-host interactions. Various studies have exploited the versatility of SPR to probe the interaction between virus and host components, including constituents of virus particles and host cellular receptors, as well as interactions between viral proteins and host immune molecules. This chapter describe作者: otic-capsule 時(shí)間: 2025-3-31 02:44 作者: 值得贊賞 時(shí)間: 2025-3-31 06:01 作者: 東西 時(shí)間: 2025-3-31 10:29
Book 20041st edition cells. Of the eight recognized g- era of vertebrate poxviruses, those belonging to the orthopoxvirus genus have been most intensively studied. This group includes variola virus, the agent of smallpox, as well as cowpox virus and vaccinia virus. Jenner’s original sma- pox vaccine, described in 1798,作者: elucidate 時(shí)間: 2025-3-31 16:24 作者: occurrence 時(shí)間: 2025-3-31 17:40
Construction of Recombinant Vaccinia Virus,with the plasmid DNA to allow homologous recombination to occur. This inactivates the endogenous TK gene-producing TK-negative virus that can be biochemically selected, and recombinants can be identified by a variety of screening methods.作者: optic-nerve 時(shí)間: 2025-4-1 01:24 作者: Tailor 時(shí)間: 2025-4-1 03:13 作者: 堅(jiān)毅 時(shí)間: 2025-4-1 08:00
An In Vitro Transcription System for Studying Vaccinia Virus Late Genes,This chapter describes a protocol that allows accurate in vitro transcription of vaccinia virus late genes. In this method, extracts are made from vaccinia virus-infected cells and used as enzyme sources to produce mRNAs from plasmid templates containing late gene promoter sequences.作者: vocation 時(shí)間: 2025-4-1 12:27 作者: Gudgeon 時(shí)間: 2025-4-1 16:37
Stuart N. IsaacsIncludes supplementary material:
