標題: Titlebook: Vaccinia Virus; Methods and Protocol Jason Mercer Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019 CRISPR.Viral [打印本頁] 作者: SCOWL 時間: 2025-3-21 18:25
書目名稱Vaccinia Virus影響因子(影響力)
書目名稱Vaccinia Virus影響因子(影響力)學科排名
書目名稱Vaccinia Virus網(wǎng)絡(luò)公開度
書目名稱Vaccinia Virus網(wǎng)絡(luò)公開度學科排名
書目名稱Vaccinia Virus被引頻次
書目名稱Vaccinia Virus被引頻次學科排名
書目名稱Vaccinia Virus年度引用
書目名稱Vaccinia Virus年度引用學科排名
書目名稱Vaccinia Virus讀者反饋
書目名稱Vaccinia Virus讀者反饋學科排名
作者: ARM 時間: 2025-3-21 21:30 作者: Diatribe 時間: 2025-3-22 02:15 作者: COUCH 時間: 2025-3-22 07:10 作者: Arthr- 時間: 2025-3-22 09:40 作者: puzzle 時間: 2025-3-22 16:00
Methods in Molecular Biologyhttp://image.papertrans.cn/v/image/980212.jpg作者: ARBOR 時間: 2025-3-22 21:03 作者: 伸展 時間: 2025-3-23 00:41
Simple, Rapid Preparation of Poxvirus DNA for PCR Cloning and Analysis, PCR amplification. This technique is reliable and robust and only requires centrifugation, detergent, and protease treatment. The resulting DNA template preparation is suitable for PCR amplification for screening viruses, cloning, transfection, and DNA sequencing.作者: constellation 時間: 2025-3-23 02:45
tungsgleichung für den dreidimensionalen Fall unter Benutzung der Methode der finiten Differenzen [1–4]. Die Aufl?sung des positiv definiten symmetrischen Bandgleichungssystems erfolgt blockweise im Kernspeicher mit einem modifizierten Algorithmus der Dreieckszerlegung nach Crout [5, 6] (SUBROUTINE 作者: contrast-medium 時間: 2025-3-23 07:58
Nouhou Ibrahim,Paula Traktmanauunternehmer Baufix zu einer übereinkunft, wonach dieser die gesamte Renovierung übernimmt. Baufix gab zun?chst ein sehr detailliertes Angebot mit Mengenans?tzen ab. Im weiteren Verlauf kam es zu einigen ?nderungen des Angebotes, die Mengenans?tze blieben unberührt. Schlie?lich kam es zum Vertragss作者: ELUC 時間: 2025-3-23 10:31 作者: glomeruli 時間: 2025-3-23 15:33
Shuai Cao,Yongquan Lin,Zhilong YangAufgaben der einfachen Statik dar. Ohne komplizierte theoretische Ableitungen werden die erforderlichen Formeln zur Bestimmung der ?u?eren und inneren Kr?fte entwickelt. Der vorliegende Teil 2 "Festigkeitslehre" erkl?rt die Beanspruchung der Bauteile. Das Buch zeigt die Bemessung von Bauteilen aus H作者: 發(fā)誓放棄 時間: 2025-3-23 19:16 作者: 猛烈責罵 時間: 2025-3-23 23:06 作者: 蓋他為秘密 時間: 2025-3-24 02:51 作者: Bravado 時間: 2025-3-24 07:23
Glennys V. Reynoso,John P. Shannon,Jeffrey L. Americo,James Gibbs,Heather D. Hickmant. Das 1984 erstmals in deutscher Sprache herausgegebene Buch kann inzwischen als Standardwerk bezeichnet werden und hat bis heute nichts von seiner Aktualit?t verloren. Der Erfolg des Buches liegt in dem schlüssigen Therapiekonzept und in der allgemeinverst?ndlichen, von unn?tigem Fachvokabular fre作者: TOXIN 時間: 2025-3-24 14:02
Book 2019ve and cutting-edge, .Vaccinia Virus: Methods and Protocols. serves as a valuable resource for scientists looking to bring new methods and procedures into their lab in order to make exciting discoveries that will continue to deepen our understanding of this fascinating virus family..作者: 范例 時間: 2025-3-24 15:17
roblem ein Datenblock zugeordnet wird, der bei Fehlern im Datenblock überlesen wird. Auβerdem werden die Daten auf formale Fehler, richtige Reihenfolge von INTEGER-Gr?βen, REAL-Gr?βen und Texten sowie Zul?ssigkeit der Randbedingungen hin getestet. Volumenelementbereiche mit gleichen thermodynamische作者: 改良 時間: 2025-3-24 19:28
Nouhou Ibrahim,Paula Traktmanzahlen, da sie nach seiner Einsch?tzung überh?ht ist. Widrig beruft sich auf seine AGB-Klauseln, wonach der Auftragnehmer, hier also Baufix, garantiert hat, da? er die Mengenans?tze nicht erh?hen werde. Baufix h?tte somit nach der Auffassung des Widrig die Erh?hungen schon aus diesem Grund nicht ber作者: 可能性 時間: 2025-3-24 23:13
Douglas W. Grosenbach,Dennis E. Hruby dem Zweck des Buches entsprechend besonders praxisnah dargestellt. Viele durchgerechne- te Beispiele erl?utern und vertiefen die Darstellung; eine sehr gro?e Zahl von übungsaufga- ben, deren L?sungen am Bandende gebracht werden, soll zur sicheren Handhabung und breiten Anwendung des Stoffes bef?hig作者: Cytology 時間: 2025-3-25 07:03
Shuai Cao,Yongquan Lin,Zhilong Yangf das Buch "Stahlbetonbau - Bemessung, Konstruktion, Ausführung" verwiesen. Die DIN-Normen sind in ihrer neuesten Fassung berücksichtigt. Eine Ausnahme bildet lediglich die Stahlbaunorm DIN 18800 vom November 1990. Diese Norm beinhaltet im Hinblick auf eine einheitliche europ?ische Normung ein neues Bemessung978-3-322-92780-4作者: 持續(xù) 時間: 2025-3-25 10:40 作者: 不整齊 時間: 2025-3-25 14:15
Quinten Kieser,Patrick Paszkowski,James Lin,David Evans,Ryan Noycereinfacht und dem Zweck des Buches entsprechend besonders praxisnah dargestellt. Viele durchgerechne- te Beispiele erl?utern und vertiefen die Darstellung; eine sehr gro?e Zahl von übungsaufga- ben, deren L?sungen am Bandende gebracht werden, soll zur sicheren Handhabung und breiten Anwendung des Stoffes bef?978-3-322-92787-3作者: 責怪 時間: 2025-3-25 16:30 作者: chemoprevention 時間: 2025-3-25 20:05 作者: 六個才偏離 時間: 2025-3-26 03:45 作者: 大方不好 時間: 2025-3-26 06:06 作者: 前兆 時間: 2025-3-26 09:32
Assessing the Structure and Function of Vaccinia Virus Gene Products by Transient Complementation, phenotype is observed following the repression, deletion, or inactivation of a particular gene product, the technique of transient complementation becomes central for further study..Simply put, transient complementation involves the transient expression of a variety of alleles of a given viral gene作者: 起草 時間: 2025-3-26 13:55 作者: Leaven 時間: 2025-3-26 19:31 作者: Vo2-Max 時間: 2025-3-26 23:46
Visualizing Poxvirus Replication and Recombination Using Live-Cell Imaging,d replication of vaccinia virus DNA, and to also detect the products of poxvirus-catalyzed recombination reactions. We describe how to use the bacteriophage lambda DNA-binding protein, cro, as a way of labeling DNA within a cell when it is conjugated to fluorescent proteins. When used in conjunction作者: Constrain 時間: 2025-3-27 02:08
Beatriz Perdiguero,Carmen Elena Gómez,Mariano Esteban作者: FLASK 時間: 2025-3-27 06:01
John P. Shannon,Olena Kamenyeva,Glennys V. Reynoso,Heather D. Hickman作者: DEMN 時間: 2025-3-27 12:06 作者: rectocele 時間: 2025-3-27 16:28 作者: hypnotic 時間: 2025-3-27 21:47 作者: 易發(fā)怒 時間: 2025-3-28 01:45
High-Content Analyses of Vaccinia Plaque Formation,t subtle virus-host interactions and their impact on virus spread during multiple rounds of infection. These include virus infectivity, mode of virus spread, virus replication rate, and spatiotemporal spread efficacy. How this underlying information can be harnessed using a high-content imaging setup is discussed here.作者: 火光在搖曳 時間: 2025-3-28 02:27
Bioluminescence Imaging as a Tool for Poxvirus Biology,the detection of viral infection sites and the quantification of viral replication in the context of tissues of a living animal. The goal of this chapter is to provide a technical background for the evaluation of poxvirus infection in cells and animals through bioluminescence imaging technology using luciferase-expressing recombinant poxviruses.作者: Pantry 時間: 2025-3-28 07:50 作者: palette 時間: 2025-3-28 12:04
Construction and Isolation of Recombinant Vaccinia Virus Expressing Fluorescent Proteins,iral–fluorescent fusion proteins by fluorescence microscopy is noninvasive and can be used to describe protein localization in live cells and track the intracellular movement of virus particles. This chapter describes a number of approaches for the construction of plasmids and subsequent generation and isolation of fluorescent recombinant viruses.作者: Immobilize 時間: 2025-3-28 14:38 作者: 周興旺 時間: 2025-3-28 19:50 作者: 強有力 時間: 2025-3-29 01:18
Super-resolution Microscopy of Vaccinia Virus Particles,l proteins on purified virions. Tens to hundreds of individual virions can be imaged in a single field of view providing data for single-particle averaging or quantitative analysis of viral protein spatial organization.作者: FLACK 時間: 2025-3-29 05:49
Intravital Imaging of Vaccinia Virus-Infected Mice,mucosa) of infected animals for MPM. Using different recombinant VACVs expressing fluorescent proteins in combination with transgenic fluorescent reporter mice, MPM imaging can be used to examine the movements, interactions, and functions of virus-infected cells or selected immune cell populations after infection.作者: SENT 時間: 2025-3-29 08:27 作者: gastritis 時間: 2025-3-29 12:36 作者: LAIR 時間: 2025-3-29 16:18 作者: addict 時間: 2025-3-29 21:14 作者: EXPEL 時間: 2025-3-30 00:27
Construction and Isolation of Recombinant Vaccinia Virus Expressing Fluorescent Proteins,creening for genetically modified strains, and molecular characterization of virus replication and spread. The detection of fluorescent proteins and viral–fluorescent fusion proteins by fluorescence microscopy is noninvasive and can be used to describe protein localization in live cells and track th作者: mettlesome 時間: 2025-3-30 04:23
Generation of Vaccinia Virus Gene Deletion Mutants Using Complementing Cell Lines, construct novel tools for poxvirus analysis. Specifically, the production and utilization of a complementing cell line expressing a poxvirus gene of interest are critical for the generation of poxvirus mutants in which essential genes are disrupted. Complementing cells are also valuable for the cha作者: Incorruptible 時間: 2025-3-30 10:05 作者: Ataxia 時間: 2025-3-30 16:22
Assessing the Structure and Function of Vaccinia Virus Gene Products by Transient Complementation, poxvirus was variola virus, the etiological agent of smallpox; today, poxviruses remain of biomedical significance, both as pathogens and as recombinant vaccines and oncolytic therapies. Vaccinia virus is the prototypic poxvirus for experimental analysis. The 195?kb dsDNA genome contains >200 genes作者: 文藝 時間: 2025-3-30 20:32
Preliminary Screening and In Vitro Confirmation of Orthopoxvirus Antivirals,us (the causative agent of smallpox) as an agent of biowarfare or bioterrorism (Henderson, 283:1279–1282, 1999). In addition to variola, monkeypox, cowpox, and vaccinia viruses are orthopoxviruses of concern to human health (Lewis-Jones, 17:81–89, 2004). Smallpox vaccination, using the closely relat作者: 六邊形 時間: 2025-3-31 00:08
Vaccinia Virus Transcriptome Analysis by RNA Sequencing, vaccinia virus and host cell. Here, we describe an RNA-Seq method for analyzing the vaccinia virus transcriptome from virus-infected HeLa cells. We pay particular attention to vaccinia virus-specific aspects of sample preparation, sequencing, and data analyses, but our method could be modified to a作者: 辮子帶來幫助 時間: 2025-3-31 04:32
Ribosome Profiling of Vaccinia Virus-Infected Cells,is method can be used to quantify gene expression at the translational level and precisely pinpoint ribosome loading onto mRNA with codon-level resolution. Genome-wide regulation of mRNA translation can also be determined if RNA-Sequencing (RNA-Seq) is carried out in parallel. Here, we describe a pr作者: 槍支 時間: 2025-3-31 05:16 作者: 一再煩擾 時間: 2025-3-31 10:40 作者: Mettle 時間: 2025-3-31 14:18 作者: Fresco 時間: 2025-3-31 19:32
High-Content Analyses of Vaccinia Plaque Formation,iter is diluted enough to allow for only few cells of the monolayer to be infected, clonal spread of infection can be detected by observing the lesion in the cell monolayer or using virus-specific staining methods. Beyond simple titration, plaque formation bares priceless underlying information abou作者: 清晰 時間: 2025-3-31 21:54
Super-resolution Microscopy of Vaccinia Virus Particles,structured illumination microscopy (SIM) and stochastic optical reconstruction microscopy (STORM) to detect fluorescently tagged or immunolabeled viral proteins on purified virions. Tens to hundreds of individual virions can be imaged in a single field of view providing data for single-particle aver作者: FLING 時間: 2025-4-1 04:42
Bioluminescence Imaging as a Tool for Poxvirus Biology,n organism and to measure therapeutic efficacy of antiviral agents in small animal models. Bioluminescence is produced by the reaction of a luciferase enzyme stably inserted into the viral genome with a defined substrate systemically delivered into the animal. The light emitted is captured allowing 作者: 爵士樂 時間: 2025-4-1 06:09 作者: 單色 時間: 2025-4-1 12:50
Book 2019ng long-standing field standards and focusing on emerging new technologies applied in the field of poxvirology. The methods and protocols have been designed with the bench scientist in mind, being presented in a fashion that makes them useful for both starting and veteran poxvirus researchers. Writt作者: scrutiny 時間: 2025-4-1 14:29
Sascha Koch,Michael Schemmannban vocabulary, facilitating visions and urban projects. This chapter addresses the reasons for, and methods of using figures of speech in urban planning, using the Netherlands as a case study. The reading of the various rhetorical devices will be based on the proposed distinction—one of the many po作者: Fluctuate 時間: 2025-4-1 22:19 作者: 品牌 時間: 2025-4-2 00:45
nlich auch im Beschaf-fungs- und Verwaltungsbereich — immer zwingender notwendig, wenn sich die Unternehmungen bei zunehmender Sch?rfe des Wettbewerbs, die sich in der Tendenz zu steigenden Vertriebskosten niederschl?gt, behaupten wollen. Dazu braucht der Unternehmer die Informationen der Marktforsc
