標(biāo)題: Titlebook: VEGF Signaling; Methods and Protocol Lorna Fiedler Book 2015 Springer Science+Business Media New York 2015 Cardiovascular Development.Lymph [打印本頁] 作者: supplementary 時(shí)間: 2025-3-21 18:20
書目名稱VEGF Signaling影響因子(影響力)
書目名稱VEGF Signaling影響因子(影響力)學(xué)科排名
書目名稱VEGF Signaling網(wǎng)絡(luò)公開度
書目名稱VEGF Signaling網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱VEGF Signaling被引頻次
書目名稱VEGF Signaling被引頻次學(xué)科排名
書目名稱VEGF Signaling年度引用
書目名稱VEGF Signaling年度引用學(xué)科排名
書目名稱VEGF Signaling讀者反饋
書目名稱VEGF Signaling讀者反饋學(xué)科排名
作者: GNAT 時(shí)間: 2025-3-21 22:57
1064-3745 details genetic mouse models, then discusses techniques for studying physiological angiogenesis in the developing mouse embryo. Written in the highly successful .Methods in Molecu978-1-4939-4370-8978-1-4939-2917-7Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: constitute 時(shí)間: 2025-3-22 00:29 作者: Nebulous 時(shí)間: 2025-3-22 07:51 作者: OMIT 時(shí)間: 2025-3-22 10:08
Vasculogenesis and Angiogenesis in VEGF Receptor-1 Deficient Miceascular quiescence in adult tissues. To facilitate further investigation, we generated a conditional knockout mouse line for VEGFR-1 and characterized angiogenesis in postnatal and adult mice, including angiogenesis in ischemic myocardium. These methods are briefly outlined in this chapter. We also 作者: 有其法作用 時(shí)間: 2025-3-22 13:45
Book 2015ediated signal transduction in the cardiovascular system that highlights recent discoveries in the field. Part III first details genetic mouse models, then discusses techniques for studying physiological angiogenesis in the developing mouse embryo. Written in the highly successful .Methods in Molecu作者: 戰(zhàn)勝 時(shí)間: 2025-3-22 20:50 作者: Seizure 時(shí)間: 2025-3-22 22:43
David J. Carr,Vedanta Mehta,Jacqueline M. Wallace,Anna L. David作者: 臭名昭著 時(shí)間: 2025-3-23 02:57
Jean-Michel Vernes,Y. Gloria Meng dampen enthusiasm at the outset. As the investigation progresses, questions inevitably arise. Descriptions of the phenomena encountered in the experiments are therefore given in the explanations. Although these were originally intended to be for the teacher‘s guidance they have been found, in fact, to be qui978-0-4191-1420-8978-94-011-6530-3作者: 偏離 時(shí)間: 2025-3-23 05:33
Yanmei Lu,Y. Gloria Menglectric field, and we shall see that this necessitates positive as well as negative carriers and therefore does not apply when the carriers are electrons only. In the latter the cathode temperature is raised. This causes the ions of the metal to oscillate and collisions between ions and electrons at作者: 殺子女者 時(shí)間: 2025-3-23 13:06 作者: 致詞 時(shí)間: 2025-3-23 14:49
Antony M. Latham,Jayakanth Kankanala,Colin W. G. Fishwick,Sreenivasan Ponnambalam admit Borel cross-sections. We will therefore assume in the rest of this chapter that . and . are free and their orbit spaces do not admit Borel cross-sections. The first important result on orbit equivalence was obtained by H. Dye [2] and the main aim of this chapter is to prove his theorem.作者: Seizure 時(shí)間: 2025-3-23 18:48
Gopika N. Nithianandarajah-Jones,Michael J. Crossological activities. In this manual the exercises are simplified and several shortcuts are taken in order to fit them into the framework of a teaching course. The introduction to each exercise gives a brief and elementary explanation of the reaction on which it is based. "Materials and Equipment" lists all ti978-3-662-00148-6作者: 描繪 時(shí)間: 2025-3-24 00:55
h vestibular and otolith control. The time constants and transfer functions for vestibular controls were obtained from the literature, and used to model the vestibular control system in a Laboratory Virtual Instrumentation Engineering Workbench (LabVIEW) Virtual Instruments program.作者: Blasphemy 時(shí)間: 2025-3-24 05:33
Vivienne C. Ho,Guo-Hua Fonges and for use in food laboratories. The author wishes to express his appreciation to the following people, who reviewed the chapters on their respective specialties: Doctors L.R. Hackler, M. Keeney, B. Love, L.M. Massey, Jr., L.R. Mattick, W.B. Robinson, R.S. Shallenberger,D.F. Splittstoesser, E. Stotz, W.L.978-94-011-7378-0978-94-011-7376-6作者: Defraud 時(shí)間: 2025-3-24 08:13 作者: Dawdle 時(shí)間: 2025-3-24 13:44 作者: 懶洋洋 時(shí)間: 2025-3-24 17:36
Jean-Michel Vernes,Y. Gloria Mengic standpoint. Fundamentals and basic concepts have often been presented with no indication of their practical appli- cations, and all too frequently they have been illustrated by artificially contrived laboratory experiments bearing little relationship to the outside world. The course comes in the 作者: 暗語 時(shí)間: 2025-3-24 19:25
Yanmei Lu,Y. Gloria Mengrs. If the enclosure contains electrons these will move to the anode, enter it, and become part of an external current, but this current will cease when all the electrons have left the enclosure. For the current to be maintained there must be a continuous supply of electrons and, clearly, these must作者: Visual-Acuity 時(shí)間: 2025-3-24 23:56
Ian Evansctional integrity over a limited range of variation in the temperature of the environment. These adaptations to thermal stress are mediated by humoral and neural pathways which are known to be susceptible to oxygen deprivation. Moreover, certain physiological responses elicited by heat or cold may b作者: insightful 時(shí)間: 2025-3-25 07:15 作者: 膽小鬼 時(shí)間: 2025-3-25 09:54 作者: morale 時(shí)間: 2025-3-25 14:54 作者: gusher 時(shí)間: 2025-3-25 18:23 作者: 通便 時(shí)間: 2025-3-25 20:33 作者: mosque 時(shí)間: 2025-3-26 01:00 作者: 惡心 時(shí)間: 2025-3-26 05:30 作者: 蜈蚣 時(shí)間: 2025-3-26 12:24
Detection and Quantification of Vascular Endothelial Growth Factor Receptor Tyrosine Kinases in Prim VEGF levels. Here, we present a robust immunoblot-based protocol for detecting and quantifying VEGFRs in human endothelial cells. Using internal and external standards, we can rapidly evaluate receptor copy number and assess how this is altered in response to the cellular environment.作者: 暫時(shí)休息 時(shí)間: 2025-3-26 14:42
Analysis of VEGF-Mediated ERK5 Activity in Endothelial Cellsta. Phos-tag? Acrylamide is a reagent that enables specific tagging of phosphorylated proteins, resulting in retarded mobility and a distinct upward band shift from the non-phosphorylated protein following SDS-PAGE. Here, we describe the details of Phosphate affinity SDS-PAGE of ERK5 using acrylamide-pendant Phos-tag?.作者: 細(xì)微的差異 時(shí)間: 2025-3-26 20:22
VEGF Gene Transfer to the Utero-Placental Circulation of Pregnant Guinea Pigs to Enhance Fetal Growt viruses with the host tissue. Our studies have shown that adenoviral gene delivery using pluronic gel resulted in the highest transduction efficiency compared to intra-arterial administration or external administration in PBS as a vehicle, when measured by X-gal staining, immunohistochemistry, or Western blotting.作者: gusher 時(shí)間: 2025-3-26 21:39
Generation of Targeted Mutations in Zebrafish Using the CRISPR/Cas Systemrategy to facilitate the synthesis of sgRNA. The system allows biallelic inactivation of multiple genes simultaneously by co-injecting a mix of sgRNAs with a single Cas9 construct. This flexible strategy of gene inactivation provides an efficient way to interrogate gene functions and genetic interactions in zebrafish.作者: 安慰 時(shí)間: 2025-3-27 05:01
Quantitation of Circulating Neuropilin-1 in Human, Monkey, Mouse, and Rat Sera by ELISAl-length NRP1 ECD has also been identified in human and animal sera. Here, we describe primate and rodent NRP1 ELISAs that measure total circulating NRP1 including soluble NPR1 and NRP1 ECD in human, monkey, mouse, and rat sera.作者: 證實(shí) 時(shí)間: 2025-3-27 05:59 作者: 泛濫 時(shí)間: 2025-3-27 12:19
VEGF Splicing and the Role of VEGF Splice Variants: From Physiological-Pathological Conditions to Spn since its expression is controlled at all possible steps including transcription, mRNA stability, translation, and pre-mRNA splicing. The latter form of molecular regulation is probably the least studied. This field has been neglected; yet different forms of VEGF with different sizes and different作者: BLAZE 時(shí)間: 2025-3-27 13:52
Detection and Quantification of VEGF Isoforms by ELISAs. The human VEGF gene has eight exons. Different VEGF isoforms are expressed via alternative RNA splicing and VEGF. and VEGF. are the major isoforms present in human tissues. The exact roles of these different VEGF isoforms are not totally clear. Assays to detect specific VEGF isoforms in biologica作者: Gentry 時(shí)間: 2025-3-27 20:37 作者: 抗原 時(shí)間: 2025-3-28 01:36 作者: Intractable 時(shí)間: 2025-3-28 04:21
Induction of VEGF Secretion in Cardiomyocytes by Mechanical Stretchjecting cells to in vitro mechanical stretch can mimic cellular responses to changes in the rigidity of the extracellular matrix. Here we describe an in vitro model system that mimics stretch overload in vivo. In this stretch-mediated hypertrophy model, adult rat cardiomyocytes attached to laminin-c作者: 糾纏 時(shí)間: 2025-3-28 07:36 作者: 吼叫 時(shí)間: 2025-3-28 13:37 作者: 開始沒有 時(shí)間: 2025-3-28 14:34 作者: Limerick 時(shí)間: 2025-3-28 21:24
Analysis of VEGF-Mediated ERK5 Activity in Endothelial Cellspathway. It is ubiquitously expressed in mammalian cells and is activated by a number of growth factors. Gene knockout studies in mice have shown a critical role for ERK5 cardiovascular development and vascular integrity. Current methods to detect ERK5 activation in cells have relied on in vitro kin作者: 隱藏 時(shí)間: 2025-3-29 00:30 作者: Deduct 時(shí)間: 2025-3-29 03:09 作者: DNR215 時(shí)間: 2025-3-29 09:38
Vasculogenesis and Angiogenesis in VEGF Receptor-1 Deficient Micetor (PlGF). VEGFR-1 is an enigmatic molecule whose precise role in postnatal angiogenesis remains controversial. Although many postnatal and adult studies have been performed by manipulating VEGFR-1 ligands, including competitive binding by truncated VEGFR-1 protein, neutralization by antibodies, or作者: 欲望小妹 時(shí)間: 2025-3-29 14:13
The Embryonic Mouse Hindbrain and Postnatal Retina as In Vivo Models to Study Angiogenesisnd wound healing, but is also associated with different diseases such as cancer and neovascular eye disease. Accordingly, elucidating the molecular and cellular mechanisms of angiogenesis has the potential to identify new therapeutic targets to stimulate new vessel formation in ischemic tissues or i作者: peak-flow 時(shí)間: 2025-3-29 19:20 作者: 完全 時(shí)間: 2025-3-29 21:40 作者: Ferritin 時(shí)間: 2025-3-30 01:54
Generation of Targeted Mutations in Zebrafish Using the CRISPR/Cas Systemegy in zebrafish using a clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein (Cas) system. By injecting two simple in vitro-synthesized components [Cas9 mRNA and single guide (sgRNA)] into one-cell-stage embryos, mutations of the target gene could be efficient作者: 故意釣到白楊 時(shí)間: 2025-3-30 05:03
Book 2015 present a range of both firmly established and newly emerging technologies, including those that are amenable to aiding in drug discovery or pre-clinical investigations. Each of the three sections begins with an introductory overview followed by supporting relevant methods. A major advantage of thi作者: EXCEL 時(shí)間: 2025-3-30 11:07
Identification of Receptor Tyrosine Kinase Inhibitors Using Cell Surface Biotinylation and Affinity relevant readout of inhibitor activity. In this example, we use the VEGF-A–VEGFR-2 axis and the well-characterized tyrosine kinase inhibitor sunitinib as a working model; however this technique is highly applicable for the identification of inhibitors to other receptor tyrosine kinases.作者: 豐滿有漂亮 時(shí)間: 2025-3-30 12:25 作者: flaggy 時(shí)間: 2025-3-30 17:02
1064-3745 ation advice from the experts.This volume provides a collection of protocols for studying and manipulating VEGF signaling pathways in vitro and in vivo, and in particular, aims to present a range of both firmly established and newly emerging technologies, including those that are amenable to aiding 作者: exclusice 時(shí)間: 2025-3-30 20:55
Chromatin Immunoprecipitation Assay: Examining the Interaction of NFkB with the VEGF Promotern cofactors, DNA replication factors, and DNA repair proteins. Here we describe a useful ChIP-qPCR protocol to examine the interaction of NFkB with the VEGF promoter in adult rat primary cardiomyocytes that have been mechanically stretched after attaching to the extracellular matrix protein laminin.作者: 敵意 時(shí)間: 2025-3-31 02:27 作者: 去世 時(shí)間: 2025-3-31 06:49 作者: minimal 時(shí)間: 2025-3-31 10:24 作者: 胖人手藝好 時(shí)間: 2025-3-31 13:21
