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標(biāo)題: Titlebook: Ultrastructure Techniques for Microorganisms; Henry C. Aldrich,William J. Todd Book 1986 Plenum Press, New York 1986 Seen.antibody.biology [打印本頁]

作者: sesamoiditis    時間: 2025-3-21 18:15
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作者: FEAS    時間: 2025-3-21 23:27
Low-Temperature Embedding,ow temperature should reduce the extent of conformational changes (i. e., rearrangement of the peptide chains) that are due to the actions of organic liquids, (3) complete dehydration should be avoided to maintain hydration shells, and (4) the specimen environment should be polar.
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作者: 是比賽    時間: 2025-3-22 14:16

作者: 可憎    時間: 2025-3-22 19:56
Computer Analysis of Ordered Microbiological Objects, image analysis are needed to extract as much reliable information as possible, for example, about the position of individual protein subunits within an assembly or to give an indication of subunit size and shape.
作者: Compassionate    時間: 2025-3-23 00:42

作者: INTER    時間: 2025-3-23 04:03
X-Ray Microanalysis,be obtained. With the new minicomputer-based systems, biologists can obtain usable results with much less effort, and the technique is thereby more accessible to the average researcher and requires less time investment.
作者: floodgate    時間: 2025-3-23 07:02

作者: 原諒    時間: 2025-3-23 12:08
Secrets of Successful Embedding, Sectioning, and Imaging, Development of an effective embedding protocol often meant success or failure of a research project. However, as microbiologists have gained more experience with a variety of embedding resins, it has become clear that we must address criteria beyond success or failure of penetration. Choice of embe
作者: backdrop    時間: 2025-3-23 13:57

作者: BLAZE    時間: 2025-3-23 21:58
Freeze-Fracture (-Etch) Electron Microscopy, interested in ultrastructure. First, because FEM is a replicating technique, it can provide both face-on as well as cross-sectional views of cellular components exposed by the fracturing of frozen cells. Thus, the investigator can visualize the morphology and distribution of specific components bot
作者: kindred    時間: 2025-3-23 22:15
Preparation of Freeze-Dried Specimens for Electron Microscopy,ntrolled sublimation. In the simplest case, freeze-drying is followed directly by observation in a transmission electron microscope. The freeze-drying step can also be incorporated into a wide range of more complex processing protocols, which we will refer to briefly, directing the reader to recent
作者: 膽汁    時間: 2025-3-24 03:33
Low-Temperature Embedding,s are subjected to the rigors of chemical fixation, dehydration in organic solvents, and embedment in various plastics and resins (most involving high-temperature curing); (2) distortion generated during sectioning; and (3) beam damage during observation. Suitable preparation methods must be develop
作者: insular    時間: 2025-3-24 08:38
High-Voltage Electron Microscopy,h higher than those used by conventional transmission (CTEM) or scanning-transmission (CSTEM) instruments. HVEMY requires a specialized instrument, commonly referred to as a high-voltage electron microscope (HVEM), that can operate at accelerating potentials of 1 MV or higher. These HVEMs differ con
作者: Ccu106    時間: 2025-3-24 11:51
Computer Analysis of Ordered Microbiological Objects,ion of micrographs has given considerable insight into the structures being investigated. However, to obtain high-resolution information from electron micrographs of microbiological material, the simple, subjective methods generally used to interpret images are often inadequate. Objective methods of
作者: 不透氣    時間: 2025-3-24 17:06
Digitizing and Quantitation,er as possible, the volume, area, number, and size of cells or components of cells derived from the analysis of two-dimensional images of sectioned material by the use of relatively simple to moderately complex techniques and equipment.
作者: 饒舌的人    時間: 2025-3-24 19:09
Localization of Carbohydrate-Containing Molecules,ed into several broad categories based on their general composition and would include the polysaccharides (both homo- and heteropolymers), glycoproteins, glycolipids, and proteoglycans. These macromolecules, especially those exposed at the cell surface, have taken on considerable importance in the s
作者: altruism    時間: 2025-3-24 23:21
Cytochemical Techniques for the Subcellular Localization of Enzymes in Microorganisms,omori’s (1952) for acid phosphatase activity, which had as end products heavy metal ions with sufficient mass to scatter electrons, were directly applicable for electron microscopy if noncoagulative fixatives were used. Introduction of catalytic osmiophilic polymer generation (Hanker .., 1972a) made
作者: 弄臟    時間: 2025-3-25 04:37

作者: Emmenagogue    時間: 2025-3-25 07:33
Colloidal Gold Labels for Immunocytochemical Analysis of Microbes,nd spatial orientation of the components studied often accelerates problem solving. Research on membrane structure and function, cell growth and development processes, and mechanisms of microbial pathogenesis is particularly likely to benefit from a visual determination of the position and movements
作者: mitten    時間: 2025-3-25 12:28
X-Ray Microanalysis,th scanning and transmission electron microscopes. Prior to that time, considerable training and experience were required before usable results could be obtained. With the new minicomputer-based systems, biologists can obtain usable results with much less effort, and the technique is thereby more ac
作者: Legion    時間: 2025-3-25 17:42

作者: 你敢命令    時間: 2025-3-25 20:00
is or her own immediate field. On the other hand, most benefits to modem microbiology are obtained when a broad spectrum of scientific approaches can be focused on a problem. In early studies, electron microscopy was pivotal in understanding bacterial and viral morphology, and we still feel that we
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作者: 珊瑚    時間: 2025-3-26 04:33

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H. C. Aldrich,H. H. Mollenhauero pharmaceutical and biomedical applications. Applications mentioned in this book include electro sensors, capacitors, electromechanical actuators, and even artificial muscles..978-3-319-37278-5978-3-319-25322-0Series ISSN 2364-1878 Series E-ISSN 2364-1886
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作者: 抗原    時間: 2025-3-26 19:34
s in their generality. A chapter on methods currently used in this field is also included. There are also chapters on theoretical and classification aspects of 978-1-4899-2301-1978-1-4899-2299-1Series ISSN 0093-6286
作者: insecticide    時間: 2025-3-26 21:34
Harvey C. Hochydrophobicity, functional groups, and flexibility of the polymer chain structure and chemical properties such as chemical reactivity, toxicity, biocompatibility, chirality, adsorption capacities, chelation, and polyfunctionality of polymeric materials are also discussed.
作者: DEFT    時間: 2025-3-27 02:44
Russell L. Chapman,L. Andrew Staehelinlock times (induction periods) are dependent upon osmarin composition, the solution concentration, pH, oxygen pressure and the temperature. The reactions are overall first order in oxygen pressure and fractional order for both hydroxide and osmium concentration. Estimates of activation energies are 10–15 Kcal/mole (41–63 KJ/mole).
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作者: fidelity    時間: 2025-3-27 17:16
Edwin S. Boatmanf drugs, and the mechanism of the drug delivery towards solid tumours by active and passive targeting. Recent innovations, applications and future progress in cancer therapy using multiple drug regimens are also discussed.
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作者: Daily-Value    時間: 2025-3-28 00:13

作者: 嘮叨    時間: 2025-3-28 03:39
Alan Beckett,Nick D. Readant similarities and much of the current knowledge of the mechanisms of dispersion polymerization has derived from that in emulsion polymerizations, an area where a great deal of fundamental research has been conducted and continues to be conducted. How the two types of polymerization came together historically is described below.
作者: Ceremony    時間: 2025-3-28 10:17
Gregory W. Erdoshen zwei bestimmten funktionellen Gruppen dargestellt sind (z. B. Veresterung einer Hydroxyl- mit einer Carboxyl-Gruppe, Amidbildung zwischen einer Amino- und einer Carboxyl-Gruppe usw.), auch in quantitativer kinetischer Hinsicht gleich sind..
作者: 我沒有強迫    時間: 2025-3-28 13:10

作者: Aura231    時間: 2025-3-28 18:26
Freeze-Fracture (-Etch) Electron Microscopy,and FEM data. Third, when coupled with a purely physical specimen fixation method (i.e., cryofixation), FEM can completely eliminate the need for chemical fixation and is thus extremely valuable for the identification of artifactual structures in electron microscopy (EM) images. Furthermore, because
作者: 注入    時間: 2025-3-28 19:21

作者: 四指套    時間: 2025-3-28 22:54
Cytochemical Techniques for the Subcellular Localization of Enzymes in Microorganisms,ture and precipitation of products; (2) ferricyanide reduction and product amplification; and (3) oxidative polymerization of diaminobenzidine. Thus, this discussion is not exhaustive of all techniques used for microorganisms but is illustrative of rationales used in attempting to identify sites of
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作者: 聽覺    時間: 2025-3-29 09:42

作者: Extort    時間: 2025-3-29 12:02
Preparation of Microfungi for Scanning Electron Microscopy,
作者: aristocracy    時間: 2025-3-29 17:31
tions for membrane-moderated devices. As mentioned in the previous chapter, conventional membrane separation techniques has opened new horizons in separation Science. These young processes are nicely employed in concentration, purification, separation, fractionation or steril filtration of effluents
作者: hermitage    時間: 2025-3-29 21:34
Alan Beckett,Nick D. Readrficially correct if not conceptually. Generally, these refer to the initial state of the system prior to polymerization while some also describe the final state after the monomer has been converted to polymer (i.e., bulk, solution, and suspension). On the other hand, the three types of emulsions ar
作者: Anthropoid    時間: 2025-3-30 02:56
William J. Toddcs. The success of gene therapy is largely dependent on the development of safe and effective gene delivery vectors for transporting geneticmaterial fromthe blood streamto the cytoplasm or nucleus. Current gene vectors can be divided into viral and non-viral vectors. Although non-viral gene delivery
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作者: 思考才皺眉    時間: 2025-3-30 13:56
e framework of the 1983 Fall Meeting of the American Chemical Society. At the First Symposium in 1977, the literature in this field could be encompassed in a single volume. To- day, that is no longer possible. The field of Polymeric Liquid Crystals grew, and continues to grow, at a very rapid pace.
作者: Ingenuity    時間: 2025-3-30 17:46
Harvey C. Hochmaterials are mass spectrometry (MS), nuclear magnetic resonance (NMR), and gel permeation chromatography (GPC), which are used for measuring mass-to-charge ratio (m/z) of analyte ions. Other methods used for this purpose include matrix-assisted laser desorption/ionization (MALDI), electrospray ioni
作者: Anecdote    時間: 2025-3-31 00:24
Russell L. Chapman,L. Andrew Staehelineroxide ions, and have activities which are comparable to those measured for peni-cillamine. In an assay which measures the capacity of compounds to protect porcine elastase inhibitor from deacti-vation by superoxide ions generated by stimulated neutro-phils, osmarins preserve up to 70% of the enzym
作者: Highbrow    時間: 2025-3-31 02:42
Thomas H. Giddings Jr.,George P. Wrayphilic blocks), having a core-shell framework proven as if one of the most efficient carriers for drugs and nucleic acids. The three major pillars for preferring this versatile group of drug carriers are their capability to cater hydrophobic drug candidates, biocompatibility, and in vivo stability.
作者: 滑動    時間: 2025-3-31 09:03
Barbara L. Armbruster,Edward Kellenbergericles for veterinary medical applications.Examines the incid.This book features an in-depth examination of the ongoing problem of bovine mastitis and the potential solutions offered by polymer nanoparticles. With extensive research and analysis, the book delves into the causes and consequences of bo
作者: 雜役    時間: 2025-3-31 10:39
Sandra A. Nierzwicki-Bauerlently to a hydrophilic (wettable) matrix such as is provided by porous glass, polyacrylamide, cellulose or agarose (for comparison of the properties of these materials see ref. 1). For the present investigations various n-alkylamines or ω-phenyl-n-alkylamines were attached to agarose (Sepharose CL-
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作者: mortuary    時間: 2025-3-31 17:49
Gregory W. Erdos, NaC1) hauptvalenzm??ig verknüpft werden, sind in gr??erer Zahl aus der organischen Chemie bekannt; die Bildung von Estern, Anhydriden, ?thern, Amiden, u. ?. sind Beispiele. Enth?lt jedes der beteiligten Moleküle mindestens zwei zur Kondensation f?hige Gruppen, so k?nnen durch solche Kondensationsr
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