標(biāo)題: Titlebook: SILAC; Methods and Protocol Jose L. Luque-Garcia Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive license to Spri [打印本頁] 作者: microbe 時間: 2025-3-21 16:50
書目名稱SILAC影響因子(影響力)
書目名稱SILAC影響因子(影響力)學(xué)科排名
書目名稱SILAC網(wǎng)絡(luò)公開度
書目名稱SILAC網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱SILAC被引頻次
書目名稱SILAC被引頻次學(xué)科排名
書目名稱SILAC年度引用
書目名稱SILAC年度引用學(xué)科排名
書目名稱SILAC讀者反饋
書目名稱SILAC讀者反饋學(xué)科排名
作者: FISC 時間: 2025-3-21 21:04 作者: Insufficient 時間: 2025-3-22 02:49 作者: Nmda-Receptor 時間: 2025-3-22 05:19 作者: Tortuous 時間: 2025-3-22 12:27 作者: 郊外 時間: 2025-3-22 15:31
So-Fong Cam Ngan,Neil E. McCarthy,Siu Kwan Szebe substituted by a component sensitive to pH, redox potential, electron current, spectral pattern, etc. The functional group may be substituted by, for example, cyclodextrin, micelles, membranes, polypeptides, etc. Furthermore, there exist many methods other than the covalent bond to transduce the 作者: immunity 時間: 2025-3-22 21:07 作者: 巧辦法 時間: 2025-3-23 00:29 作者: fabricate 時間: 2025-3-23 01:57 作者: Derogate 時間: 2025-3-23 08:15
María Luisa Hernáez,Concha Gilell-organized data with up-to-date information on geographic distribution of particular aphid species and their host relationships. It will be a useful addition to the libraries of institutions and researchers interested in biosystematics, ecology, genetics and phylogeny of aphids, as well as in biodiversity and plant protection..978-1-4020-8286-3作者: 不持續(xù)就爆 時間: 2025-3-23 13:19 作者: Anthem 時間: 2025-3-23 16:33 作者: Cumulus 時間: 2025-3-23 18:37
Tomonori Tamura,Itaru Hamachiell-organized data with up-to-date information on geographic distribution of particular aphid species and their host relationships. It will be a useful addition to the libraries of institutions and researchers interested in biosystematics, ecology, genetics and phylogeny of aphids, as well as in biodiversity and plant protection..978-1-4020-8286-3作者: Adulate 時間: 2025-3-23 23:53
Alexis Gonneaud,Claude Asselin,Véronique Giroux,Fran?ois-Michel Boisvertell-organized data with up-to-date information on geographic distribution of particular aphid species and their host relationships. It will be a useful addition to the libraries of institutions and researchers interested in biosystematics, ecology, genetics and phylogeny of aphids, as well as in biodiversity and plant protection..978-1-4020-8286-3作者: hauteur 時間: 2025-3-24 04:31 作者: 和藹 時間: 2025-3-24 09:43
Guillermo Aragoneses-Cazorla,Andres Machuca,M. Pilar Buendia-Nacarino,Daniela S. Anuncia??o,Estefanimuscle layer, subtegumental cell layer, and distribution of frontal gland showed as marked features. The physiological, biochemical, and molecular phylogenic features have been applied to species identification. We have tried to analyze the trace element content, amino acid content, isozyme pattern,作者: Ointment 時間: 2025-3-24 13:53 作者: 附錄 時間: 2025-3-24 16:29
Jolene M. Duda,Stefani N. Thomasof data, it may species, a characteristic that led J. H. Fabre to elab- have been tempting to draw far-reaching conclu- orate the notion of the ‘insects‘ botanical instinct‘ a sions. However, the author has been very careful in century ago. doing this.978-94-010-7336-3978-94-009-1874-0作者: 交響樂 時間: 2025-3-24 19:21 作者: FOR 時間: 2025-3-25 02:53 作者: ureter 時間: 2025-3-25 04:25
Alistair Langlands,Hamze Beati,H.- Arno J. Müllerrganized from both, insect and plant viewpoints. Results are analyzed and illustrated by graphs and diagrams. The main impact of the monograph is seen in entomology, phytopathology, forestry, agriculture ecology and biocontrol.978-3-319-36189-5978-3-319-08410-7作者: 憎惡 時間: 2025-3-25 10:34
Aline R. D?rrbaum,Erin M. Schuman,Julian D. Langer作者: photopsia 時間: 2025-3-25 14:50
1064-3745 expertsThis detailed volume explores the most recent methodologies and applications of stable isotope labeling by amino acids in cell culture (SILAC). With the development of new technologies in the field of mass spectrometry and bioinformatics, SILAC-based methods have become the first tool of cho作者: acrophobia 時間: 2025-3-25 19:37
Book 2023 necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.?.Authoritative and practical, .SILAC: Methods and Protocols. serves as an ideal guide for researchers working in the area of functional proteomics and other aspects of protein science..作者: 征服 時間: 2025-3-25 21:57
Identification of Protein Interaction Partners in Bacteria Using Affinity Purification and SILAC Quanalysis and interpretation of MS data to identify and select the specific partners interacting with the protein of interest. As an example, this workflow is applied to the discovery of potential interaction partners of glyceraldehyde-3-phosphate dehydrogenase in the gram-negative bacterium ..作者: 荒唐 時間: 2025-3-26 01:20 作者: 逃避責(zé)任 時間: 2025-3-26 07:03 作者: 概觀 時間: 2025-3-26 09:00
Profiling of Secreted Proteins in Serum-Containing Media Using BONCAT and Pulsed SILAC,ome in SCM. In this protocol, the secretome of SFM is compared with that of SCM to confirm the effect of FBS. Additionally, for mass spectrometric data processing, we provide parameters that increase true positives and decrease both false positives and false negatives.作者: 捐助 時間: 2025-3-26 16:24
pSILAC-Based Determination of Cellular Protein Sorting into Extracellular Vesicles,pSILAC-based approaches can be used to pulse/trace the origins of EV protein content and thereby provide valuable insight into vesicle biology and likely effects on intercellular communication in diverse settings.作者: 小教堂 時間: 2025-3-26 19:18
Stable Isotope Labeling by Amino Acids and Bioorthogonal Noncanonical Amino Acid Tagging in Cultureled. When combined with bioorthogonal noncanonical amino acid tagging (BONCAT), it allows for comparative proteomic analysis of de novo protein synthesis. Here we describe protocols that utilize the multiplex SILAC labeling strategy for primary cultured neurons to study steady-state and nascent proteomes.作者: cinder 時間: 2025-3-26 22:35
Heavy Methyl SILAC Metabolic Labeling of Human Cell Lines for High-Confidence Identification of R/Kh-confidence identification of in vivo methyl-peptides by MS-based proteomics. We provide a general protocol that covers the steps of heavy methyl labeling of cultured cells, protein sample preparation, LC-MS/MS analysis, and downstream computational analysis of the acquired MS data.作者: dilute 時間: 2025-3-27 02:12
SILAC-Based Quantitative Phosphoproteomics in Yeast,e..The combination of SILAC-based quantitation with phosphopeptides enrichment by TiO. in a batch that enables measurement of protein posttranslational modifications is a powerful application to analyze the global phosphoproteome for studies in signaling pathways.作者: fleeting 時間: 2025-3-27 06:08 作者: 天空 時間: 2025-3-27 11:39
SILAC-Based Quantitative Proteomic Analysis of Drosophila Embryos,cy of over 92%. In combination with genetic selection markers, this method permits the quantification of translational and posttranslational changes in embryos mutant for developmental and disease-related genes.作者: VEN 時間: 2025-3-27 16:49
Use of Nuclear and Chromatin Enrichment Procedures for Quantitation of Yeast DNA Replication Protei)-based quantitative proteomics. Here I describe a detailed methodology for SILAC labeling of budding yeast ., then nuclear isolation and chromatin preparation from synchronized yeast cells, prior to quantitative proteomic analysis of DNA replication proteins.作者: Ptsd429 時間: 2025-3-27 21:32
Combination of Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) and Substrate Trappine these classes of proteins in affinity-purified mixtures. Here we describe the use of stable isotope labeling by amino acids in cell culture, substrate trapping, and mass spectrometry to enable the objective identification of the components of affinity-purified protein complexes.作者: 外向者 時間: 2025-3-28 01:22
Dynamic SILAC to Determine Protein Turnover in Neurons and Glia,fferent rates depending on various factors including cell type, subcellular localization, cellular environment, and neuronal activity. Here we describe a workflow for the analysis of protein synthesis, degradation, and turnover in primary cultured rat neurons and glia using dynamic/pulsed SILAC and mass spectrometry.作者: adroit 時間: 2025-3-28 03:08 作者: 暫時中止 時間: 2025-3-28 09:22 作者: chance 時間: 2025-3-28 13:59 作者: ALLAY 時間: 2025-3-28 15:20
Identification of Protein Interaction Partners in Bacteria Using Affinity Purification and SILAC Qumatic detection at near physiological conditions. The addition of a quantitative proteomic method, like SILAC metabolic labeling, allows the elimination of non-specifically bound contaminants which greatly increases the confidence of the identified interaction partners. Compared to eukaryotic cells,作者: macrophage 時間: 2025-3-28 19:29
pSILAC-Based Determination of Cellular Protein Sorting into Extracellular Vesicles,nvironmental cues can modify EV loading with proteins derived from the plasma membrane via endocytosis, obtained from the preexisting cytosolic pool via active sorting, or packaging with newly synthesized proteins drawn from trans-golgi networks. Given the major impact these pathways exert on EV con作者: TERRA 時間: 2025-3-28 23:27
Comparative SUMO Proteome Analysis Using Stable Isotopic Labeling by Amino Acids (SILAC),, DNA repair, transcriptional control, and chromatin remodeling. Interestingly, various stress conditions such as heat shock, oxidative stress, and ischemia promote global changes in sumoylation in different cells or tissues. However, due to limitations in either abundance or steady state sumoylatio作者: 救護(hù)車 時間: 2025-3-29 06:49
A Super-SILAC Approach for Profiling Histone Posttranslational Modifications,physiological and pathological processes. During the last decade, mass spectrometry (MS) has emerged as the most accurate and versatile tool to quantitate histone PTMs. Stable-isotope labeling by amino acids in cell culture (SILAC) is an MS-based quantitation strategy involving metabolic labeling of作者: Formidable 時間: 2025-3-29 09:02
SILAC-Based Quantitative Phosphoproteomics in Yeast, evaluation of phosphopeptide enrichment, and sample preparation and analysis by high-resolution LC-MS/M, identification of phosphosites, and quantification methods are described..We report methods for the application of double SILAC to yeast using a combination of labeled lysine and labeled arginin作者: Tempor 時間: 2025-3-29 14:36
Phosphotyrosine Profiling Using SILAC, phosphotyrosine profiling can reveal tyrosine kinase signaling activity in cells. Using quantitative proteomics strategies such as stable isotope labeling with amino acids in cell culture (SILAC) allows comparison of tyrosine kinase signaling activity across two to –three different conditions. In t作者: 試驗(yàn) 時間: 2025-3-29 17:43
Chemoproteomic Profiling of Geranyl Pyrophosphate-Binding Proteins,n combination with stable isotope labeling by amino acids in cell culture (SILAC), for the proteome-wide profiling of geranyl pyrophosphate (GPP)-binding proteins. After labeling using a desthiobiotin-GPP acyl phosphate probe, desthiobiotin-conjugated peptides of GPP-binding proteins could be enrich作者: 反對 時間: 2025-3-29 20:01
Quantitative Analysis of the Endoplasmic Reticulum-Associated Proteins Using ER-Localizable Reactivesis, membrane trafficking, and storage of Ca.. Therefore, global profiling of ER-associated proteins should be invaluable for understanding these biological processes. However, the difficulty of isolating the intact ER hampered proteome-wide analysis of ER proteins. This chapter describes a chemopr作者: Addictive 時間: 2025-3-30 03:23
Workflow for Quantitative Proteomic Analysis of Intestinal Organoids Using SILAC,s in different conditions simultaneously. In recent years, 3D cell growth culture conditions have been developed to establish intestinal organoids from isolated crypts, which mimic the intestine’s cell composition and organization. Organoids, isolated from normal or diseased tissues, can be used to 作者: Polydipsia 時間: 2025-3-30 06:52
Stable Isotope Labeling by Amino Acids and Bioorthogonal Noncanonical Amino Acid Tagging in Culturel culture (SILAC) requires nearly complete metabolic labeling of proteins and therefore is difficult to apply to cultured primary neurons, which do not divide in culture. In a multiplex SILAC strategy, two different sets of heavy amino acids are used for labeling cells for the different experimental作者: 谷物 時間: 2025-3-30 09:05 作者: 甜食 時間: 2025-3-30 15:39 作者: FILLY 時間: 2025-3-30 19:22 作者: Bridle 時間: 2025-3-30 22:52 作者: 和藹 時間: 2025-3-31 03:13 作者: Vertical 時間: 2025-3-31 05:50
Profiling of Secreted Proteins in Serum-Containing Media Using BONCAT and Pulsed SILAC, past decade, most studies on this topic have utilized serum-free medium (SFM). However, fetal bovine serum (FBS) is the most widely used serum supplement for cell culture, and secretome analysis using serum-containing medium (SCM) is important to identify proteins secreted under realistic condition作者: 珊瑚 時間: 2025-3-31 12:52
Book 2023he development of new technologies in the field of mass spectrometry and bioinformatics, SILAC-based methods have become the first tool of choice in quantitative proteomics either on its own or in combination with other more recently developed approaches, which is elucidated in the chapters of this 作者: gain631 時間: 2025-3-31 14:41
Dorothea Anrather,Silvia Bagelova Polakova,Lubos Cipak,Juraj Gregans. When bound to a macromolecular chain, their binding properties can change significantly as a result of cooperative binding effects involving adjacent ligands or comonomer substituents, microenvironmental effects caused by the polymer backbone or by neighboring groups, conformational restrictions 作者: 厚顏無恥 時間: 2025-3-31 21:09
Monika Kopeckova,Marek Link,Ivona Pavkovan the initial stage of the crown chemistry the syntheses of the crown compounds and the characterizations of their metal complexes were the main objects of the investigation, the functional facet of crown ethers has been left less exploited for a long time. To let the crown ether work — this is the 作者: Matrimony 時間: 2025-3-31 22:49
So-Fong Cam Ngan,Neil E. McCarthy,Siu Kwan Szen the initial stage of the crown chemistry the syntheses of the crown compounds and the characterizations of their metal complexes were the main objects of the investigation, the functional facet of crown ethers has been left less exploited for a long time. To let the crown ether work — this is the 作者: 相容 時間: 2025-4-1 05:53 作者: NOT 時間: 2025-4-1 08:49 作者: 組裝 時間: 2025-4-1 12:02 作者: 史前 時間: 2025-4-1 15:18 作者: wreathe 時間: 2025-4-1 18:40
