標(biāo)題: Titlebook: Rice Genome Engineering and Gene Editing; Methods and Protocol Anindya Bandyopadhyay,Roger Thilmony Book 2021 Springer Science+Business Med [打印本頁] 作者: Reagan 時間: 2025-3-21 18:45
書目名稱Rice Genome Engineering and Gene Editing影響因子(影響力)
書目名稱Rice Genome Engineering and Gene Editing影響因子(影響力)學(xué)科排名
書目名稱Rice Genome Engineering and Gene Editing網(wǎng)絡(luò)公開度
書目名稱Rice Genome Engineering and Gene Editing網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Rice Genome Engineering and Gene Editing被引頻次
書目名稱Rice Genome Engineering and Gene Editing被引頻次學(xué)科排名
書目名稱Rice Genome Engineering and Gene Editing年度引用
書目名稱Rice Genome Engineering and Gene Editing年度引用學(xué)科排名
書目名稱Rice Genome Engineering and Gene Editing讀者反饋
書目名稱Rice Genome Engineering and Gene Editing讀者反饋學(xué)科排名
作者: moratorium 時間: 2025-3-21 23:11 作者: Fulminate 時間: 2025-3-22 01:18 作者: Mercantile 時間: 2025-3-22 05:02 作者: Trypsin 時間: 2025-3-22 09:58 作者: cruise 時間: 2025-3-22 16:38 作者: PATRI 時間: 2025-3-22 20:08
1064-3745 ome Engineering and Gene Editing: Methods and Protocols. serves as a valuable resource for researchers worldwide striving to further their efforts on advancing research and producing genetically improved rice varieties..978-1-0716-1070-1978-1-0716-1068-8Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Enteropathic 時間: 2025-3-22 22:32 作者: Arresting 時間: 2025-3-23 04:35 作者: 改正 時間: 2025-3-23 08:06 作者: Terrace 時間: 2025-3-23 10:45 作者: largesse 時間: 2025-3-23 15:23 作者: ANTI 時間: 2025-3-23 20:23
Samson Nalapalli,Meral Tunc-Ozdemir,Yuejin Sun,Sivamani Elumalai,Qiudeng Que=t. im ..(-∞)=. befinden und solange in diesem Zustand verharren, bis die Eingangsfolgen erstmals von Null verschiedene Werte auf weisen. Wenn im folgenden vereinzelt nichtver schwindende Anfangszust?nde .(k.) mit k.>-∞ betrachtet werden, so wird dabei entweder eine vorausgegangene Erregung oder ein作者: gout109 時間: 2025-3-24 00:19
Sudheer Kumar,Neeti Sanan-Mishrat. =t., im . .(-∞) = . befinden und solange in diesem Zustand verharren, bis die Eingangsfolgen erstmals von Null verschiedene Werte aufweisen. Wenn im folgenden vereinzelt nichtverschwindende Anfangszust?nde .(k. ) mit k . > -∞ betrachtet werden, so wird dabei entweder eine vorausgegangene Erregung作者: 運動的我 時間: 2025-3-24 02:38 作者: cortisol 時間: 2025-3-24 07:47
Tanushri Kaul,Sonia Khan Sony,Nitya Meenakshi Raman,Khaled Fathy Abdel Motelb,Jyotsna Bhartien. In manchen F?llen ist ein ausreichender Gruppenlaufzeitausgleich mit Hilfe von Allp?ssen, die dem laufzeitverzerrenden Filter nachgeschaltet werden, m?glich. Auf diese Weise kann jedoch bei vertretbarer Aufwandserh?hung nur ein n?herungsweise konstanter Gruppenlaufzeitgang erzielt werden.作者: Pert敏捷 時間: 2025-3-24 12:27 作者: Noctambulant 時間: 2025-3-24 16:00
Juntao Liu,Dawei Liang,Li Yao,Ya Zhang,Chunxia Liu,Yubo Liu,Yanli Wang,Hongju Zhou,Timothy Kelliher,echnung und Erfahrung die praktische Anwendbarkeit der Beziehungsgleichungen zur Voraussage des Temperaturcharakters prüfen. Bei einer solchen Deutung entsprechen die . dem jeweiligen ?wahrscheinlichsten“ Werte und der mittlere Fehler der Beziehungsgleichung ist der mittlere Fehler, der bei der Vora作者: 暴露他抗議 時間: 2025-3-24 20:06 作者: 去世 時間: 2025-3-24 23:35
Genome Editing of Rice by CRISPR-Cas: End-to-End Pipeline for Crop Improvementcation for development of commercial crop product may require additional processes, including field trials in the target geography for evaluation of product efficacy. Evaluation of genome edited lines in controlled greenhouse/net house or open field condition requires few generations for outcrossing作者: 閃光你我 時間: 2025-3-25 04:17 作者: 為敵 時間: 2025-3-25 10:29
Qinlong Zhu,Yao-Guang Liuun-mittelbarer Bedeutung.. In diesem Zusammenhang ist es zweckm??ig, diese Gr??en aus einer gegebenen Netzwerkbeschreibung zu eliminieren. Das resultierende Gleichungssystem, das lediglich Eingangs-, Ausgangs- und Zustands-gr??en miteinander verknüpft, hei?t .. Wie bereits in Abschnitt 6. angedeutet作者: 建筑師 時間: 2025-3-25 15:40 作者: 織布機 時間: 2025-3-25 19:23
Sudheer Kumar,Neeti Sanan-Mishraunmittelbarer Bedeutung.. In diesem Zusammenhang ist es zweckm??ig, diese Gr??en aus einer gegebenen Netzwerkbeschreibung zu eliminieren. Das resultierende Gleichungssystem, das lediglich Eingangs-, Ausgangs- und Zustandsgr??en miteinander verknüpft, hei?t .. Wie bereits in Abschnitt 6. angedeutet, 作者: AV-node 時間: 2025-3-25 22:15
Shuvobrata Majumder,Karabi Datta,Swapan Kumar Dattaunmittelbarer Bedeutung.. In diesem Zusammenhang ist es zweckm??ig, diese Gr??en aus einer gegebenen Netzwerkbeschreibung zu eliminieren. Das resultierende Gleichungssystem, das lediglich Eingangs-, Ausgangs- und Zustandsgr??en miteinander verknüpft, hei?t .. Wie bereits in Abschnitt 6. angedeutet, 作者: Fissure 時間: 2025-3-26 01:32 作者: debouch 時間: 2025-3-26 07:12
Yesuf Teslim Yimam,Jianping Zhou,Sayed Abdul Akher,Xuelian Zheng,Yiping Qi,Yong Zhangnnigfaltigkeit des physischen Geschehens zurechtfinden zu k?nnen, insbesondere um darin Gesetzm??igkeiten zu erkennen, die eine Voraussage des Wetters erm?glichen, müssen wir durch geeignete Fiktionen an die Stelle der physischen Wirklichkeit die ideelle Wirklichkeit unserer eigenen Denkgesetze, die作者: Organization 時間: 2025-3-26 09:29 作者: Resection 時間: 2025-3-26 13:31 作者: 令人心醉 時間: 2025-3-26 17:59 作者: stratum-corneum 時間: 2025-3-26 21:56
TransGene Stacking II Vector System for Plant Metabolic Engineering and Synthetic Biology complex agronomic traits in plants. Here we present a high-efficiency multigene assembly and transformation vector system, TransGene Stacking II (TGS II), for these purposes. The operation process is described in detail, and the successful operation mainly depends on effective reagents, special . s作者: 咒語 時間: 2025-3-27 02:28
Morphogenic Regulators and Their Application in Improving Plant Transformationnsformation methods are the most commonly used technology but these approaches are limited to a small number of plant species with efficient transformation systems. The ideal transformation technologies are those that allow biotechnology applications across wide genetic background, especially within作者: 情愛 時間: 2025-3-27 08:53 作者: 無能的人 時間: 2025-3-27 12:26
-Mediated Transformation of Rice by , (,) Gene Containing CRISPR/Cas9 Vector .-mediated transformation (AMT) is considered a suitable gene transformation method. The AMT for .-type rice is a challenge because it is less efficient in tissue culture response than .-type rice. Here is a protocol of the AMT method that we developed for IR64 variety which has been successfully t作者: 推延 時間: 2025-3-27 15:37
Genotype-Independent Regeneration and Transformation Protocol for Rice Cultivarsnetic enhancement of crops, especially rice. The present study has been carried out in order to establish a genotype-independent regeneration and biolistic transformation protocol for rice varieties. Putative transgenic rice lines were confirmed by PCR analysis, DNA sequencing, and Southern analysis作者: 石墨 時間: 2025-3-27 21:49 作者: 夾克怕包裹 時間: 2025-3-27 23:19
Genome Editing of Rice by CRISPR-Cas: End-to-End Pipeline for Crop Improvementimal. This technology can be harnessed with ease to understand the basic genetics of a living system by altering sequence of individual genes and characterizing their functions. The precision of this technology is unparallel. It allows very precise and targeted base pair level edits in the genome. H作者: lesion 時間: 2025-3-28 05:52
Single Base Editing Using Cytidine Deaminase to Change Grain Size and Seed Coat Color in Rice focused on gene knocking out by creating indels. However, more precise genome editing tools are demanded to assist the introduction of functional single nucleotide polymorphisms (SNPs) in breeding programs. The CRISPR base editing tools were developed to meet this need. In this chapter, we present 作者: 咆哮 時間: 2025-3-28 07:41
Analysis of Off-Target Mutations in CRISPR-Edited Rice Plants Using Whole-Genome Sequencing off-target mutations due to sgRNA recognizing highly homologous DNA sequence elsewhere in the genome. Whole-genome sequencing (WGS) can be used to identify on- and off-target mutation. Here, we describe a pipeline of analyzing WGS data using a series of open source software for analysis of off-targ作者: BALK 時間: 2025-3-28 12:00
Efficient Genome Editing in Rice Protoplasts Using CRISPR/CAS9 Constructyotic cells, a very large number of derived technologies has emerged. Demonstrating and comparing the effectiveness of all these new technologies in entire plants is a long, tedious, and labor-intensive process that generally involves the production of transgenic plants and their analysis. Protoplas作者: 彩色 時間: 2025-3-28 15:24
Single Transcript Unit CRISPR 2.0 Systems for Genome Editing in Rice adopted as two of the most powerful genome editing systems in plants. Recently, we developed a single transcript unit (STU) CRISPR 2.0 toolbox for applications in plants, which contains two STU-Cas9 systems and one STU-Cas12a system. Here, we describe a detailed protocol about using the STU CRISPR 作者: 作嘔 時間: 2025-3-28 22:01
Improving a Quantitative Trait in Rice by Multigene Editing with CRISPR-Cas9e effector nuclease (TALEN), such as multiplex genome editing. With multiplex genome editing, CRISPR-Cas9 becomes a preferred SSN to edit many quantitative trait loci (QTL) simultaneously for trait improvement in major crops. A multiplexed CRISPR system is also important for deletion of a large frag作者: 太空 時間: 2025-3-29 02:34
FLP-Mediated Site-Specific Gene Integration in Rice is by using the yeast FLP-. recombination system that is efficient in directing DNA integration into the “engineered” genomic sites. The critical parameters of this method include the use of the thermostable version of FLP protein and the promoter trap design to select site-specific integration clo作者: 尖酸一點 時間: 2025-3-29 06:07 作者: inclusive 時間: 2025-3-29 07:49
Morphogenic Regulators and Their Application in Improving Plant Transformation elite germplasm of major crop species. This chapter will briefly review key regulatory genes involved in plant morphogenesis with a focus on in vitro somatic embryogenesis and their application in improving plant transformation.作者: 阻礙 時間: 2025-3-29 12:32 作者: Synapse 時間: 2025-3-29 16:05 作者: 孤僻 時間: 2025-3-29 20:46 作者: Nomadic 時間: 2025-3-30 03:41 作者: 膽小懦夫 時間: 2025-3-30 04:24 作者: 有幫助 時間: 2025-3-30 08:32 作者: 浪蕩子 時間: 2025-3-30 14:59 作者: Cumbersome 時間: 2025-3-30 20:11
Genotype-Independent Regeneration and Transformation Protocol for Rice Cultivarsistic transformation protocol for rice varieties. Putative transgenic rice lines were confirmed by PCR analysis, DNA sequencing, and Southern analysis. The transformation protocol reported here is relatively simple and consistent and can be exploited in future biotechnological investigations particularly for gene transformation studies.作者: Adulate 時間: 2025-3-30 22:28 作者: incisive 時間: 2025-3-31 04:10 作者: 口訣法 時間: 2025-3-31 05:10
Analysis of Off-Target Mutations in CRISPR-Edited Rice Plants Using Whole-Genome Sequencingols. Whole-genome single nucleotide variations (SNVs) and indels are detected by LoFreq., Mutect2, VarScan2, and Pindel. Last, SNVs and indels are compared with in silico off-target sites using Cas-OFFinder.作者: STING 時間: 2025-3-31 10:26
Efficient Genome Editing in Rice Protoplasts Using CRISPR/CAS9 Construct in protoplasts prepared from leaf tissues of 100, 9–11-day-old seedlings. CRISPR/CAS9 construct effectiveness can be clearly established within less than a week. We provide here a full protocol, from designing sgRNA to mutation analysis.作者: 鞏固 時間: 2025-3-31 15:43
Improving a Quantitative Trait in Rice by Multigene Editing with CRISPR-Cas9practiced including Golden Gate assembly. This chapter provides a detailed protocol for the construction of a T-DNA binary vector for expressing Cas9 and three sgRNAs for simultaneous targeting of three QTL genes for improving seed trait in rice.作者: Intellectual 時間: 2025-3-31 18:18
1064-3745 format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoidi978-1-4939-5729-3978-1-60761-535-4Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: CHOP 時間: 2025-3-31 23:54
The Witness-People Myth and Its Alternatives,istians. The special stature of the Jewish people was not lost in the angry disputes of the first century or the fateful parting of the ways in the second. Emerging “Christianity” of the first century . defined itself primarily in relation to mainstream Judaism and its interpretation of Jewish Scrip作者: Frisky 時間: 2025-4-1 04:56
p of employer and trade union representatives, education and training specialists, policy analysts, and government officials to discuss research and policy questions raised by training activities at the workplace. As one might expect, the discussion ranged widely and reflected many viewpoints on the relations978-94-009-8146-1978-94-009-8144-7作者: alcoholism 時間: 2025-4-1 06:34