標(biāo)題: Titlebook: Ribozyme Protocols; Philip C. Turner Book 1997 Springer Science+Business Media New York 1997 [打印本頁(yè)] 作者: 葉子 時(shí)間: 2025-3-21 18:04
書(shū)目名稱Ribozyme Protocols影響因子(影響力)
作者: 愚笨 時(shí)間: 2025-3-21 21:27 作者: BURSA 時(shí)間: 2025-3-22 02:16 作者: 柔聲地說(shuō) 時(shí)間: 2025-3-22 08:11
A Practical Method for the Production of RNA and Ribozymes,des. Although great progress has been made in improving the methods for DNA synthesis, such that production of large amounts has become commonplace, similar quantities of RNA are still difficult to obtain.作者: objection 時(shí)間: 2025-3-22 12:26
Preparation of Templates for Production of Ribozymes and Substrates, by in vitro transcription can be used as substrates in a variety of experiments ranging from splicing and translation to structural studies. This chapter describes methods for the preparation of templates suitable for in vitro transcription.作者: quiet-sleep 時(shí)間: 2025-3-22 14:57
Enzymatic Synthesis and Characterization of Unmodified Ribozymes and Substrates,s can be end-labeled. Simple procedures generate a sequence ladder to confirm colinearity of template and transcript (.,.). This is not a “mere luxury,” since examples with significant template slippage have been reported (.). If a small labeled cleavage product is produced, also the exact cleavage sites can be determined (.).作者: overhaul 時(shí)間: 2025-3-22 20:37
Minimized Hammerhead Ribozymes,form stem-loop II, and nucleotides at the 5′- and 3′-ends, which form helix I and helix III, respectively, in complex with the substrate. Such a ribozyme is shown in .. Most hammerhead ribozymes of this type are constructed with 12 nucleotides in stem-loop II, with four nucleotides in the loop and eight nucleotides forming a 4 bp double helix.作者: cancellous-bone 時(shí)間: 2025-3-22 23:47 作者: SPALL 時(shí)間: 2025-3-23 02:49 作者: 永久 時(shí)間: 2025-3-23 06:33
Qiao Yu,John M. Burkener Absenkung von Sedimenten kommt es unter zunehmenden geothermischen Einflu?, da normalerweise eine Temperaturzunahme von 3°C pro 100m Sedimentteufe (geothermischer Gradient) anzutreffen ist. Die Folge ist, da? sich das organische Material allm?hlich ver?ndert, indem nur thermisch stabile Produkte作者: 半圓鑿 時(shí)間: 2025-3-23 09:46
Arnold Hampel,Mary Beth De Young,Scott Galasinski,Andrew Siwkowski denen des kristallinen Siliziums abweichen, berücksichtigt werden. Nahezu alle diese abweichenden Eigenschaften lassen sich von einem grundlegenden Unterschied zwischen Si und GaAs ableiten: GaAs ist ein ., Si dagegen . (s. Abb. 3.2). Daraufhin verl?uft der Absorptionskoeffizient α(λ) des GaAs über作者: 狂熱文化 時(shí)間: 2025-3-23 14:16
Ribozymes,ic RNA molecules, or ribozymes, to carry out their specific reactions has led to the realization that ribozymes can easily be manipulated to act on novel substrates. These custom-designed RNAs have great potential as therapeutic agents and are becommg a powerful tool for molecular biologists.作者: 語(yǔ)言學(xué) 時(shí)間: 2025-3-23 20:13 作者: Chauvinistic 時(shí)間: 2025-3-23 22:18 作者: 寡頭政治 時(shí)間: 2025-3-24 03:10
PCR-Based Construction of Long Hammerhead Ribozymes,isense RNA containing the hammerhead catalytic core that will cleave the target of interest at a GUC sequence. All discussion will focus on the hammerhead motif, although, in principle, the hairpin motif could be incorporated instead.作者: Digest 時(shí)間: 2025-3-24 07:40 作者: grieve 時(shí)間: 2025-3-24 11:51
Ribozymes, primarily passive carriers of genetic information, can carry out some functions previously ascribed to proteins. Some RNAs are capable of acting as enzymes even in the complete absence of proteins. The reactions observed include cleaving themselves or other RNA molecules, ligation, and .-splicing. 作者: Arb853 時(shí)間: 2025-3-24 16:52
Computer-Aided Calculation of the Local Folding Potential of Target RNA and Its Use for Ribozyme Deleavable motif. In the case of hammerhead ribozymes, this is a specific base triplet. Even though it has been described that there are 12 conceivable motifs recognized by hammerhead ribozymes that are cleaved with different efficiencies (.,.), a typical long-chain target RNA provides a large number 作者: Monotonous 時(shí)間: 2025-3-24 19:04
Computational Approaches to the Identification of Ribozyme Target Sites,rofound. Although we are only beginning to understand the underlying reasons for this extreme variation, we can identify a number of variables that might well be significant in the process, and some of these can be modeled computationally. It must be emphasized, however, that only well-designed and 作者: 苦澀 時(shí)間: 2025-3-25 02:09 作者: 名義上 時(shí)間: 2025-3-25 05:04
Selection of Accessible Sites for Ribozymes on Large RNA Transcripts,However, the therapeutic target of antisense oligonucleotides and hammerhead ribozymes, large RNAs, are rich in structures, such as hairpins and pseudoknots, which may make certain target sequences inaccessible to the ribozyme (.–.). The methods described here are used in our laboratories to probe t作者: 劇毒 時(shí)間: 2025-3-25 07:48
Selection of Efficient Ribozyme Cleavage Sites in Target RNAs,ppointing failures. Even prediction of secondary structures of the target RNA by computer modeling with a more or less exact prediction of single-stranded or loop regions does not guarantee successful cleavage of the target RNA by a synthetic or an expressed ribozyme (.). Expensive screening tests w作者: 平庸的人或物 時(shí)間: 2025-3-25 14:34
Chemical Synthesis, Analysis, and Purification of Ribozymes, group. Solid-phase chemistry currently provides the most effective means for the scale-up of RNA synthesis. The biological activity of chemically synthesized RNA (.–.), equivalent to that of RNA derived by transcription methods (.–.), is contingent on efficient synthesis and purification protocols.作者: Meditate 時(shí)間: 2025-3-25 16:26
A Practical Method for the Production of RNA and Ribozymes,des. Although great progress has been made in improving the methods for DNA synthesis, such that production of large amounts has become commonplace, similar quantities of RNA are still difficult to obtain.作者: Ascribe 時(shí)間: 2025-3-25 22:18
Preparation of Templates for Production of Ribozymes and Substrates, by in vitro transcription can be used as substrates in a variety of experiments ranging from splicing and translation to structural studies. This chapter describes methods for the preparation of templates suitable for in vitro transcription.作者: 青石板 時(shí)間: 2025-3-26 03:35
Enzymatic Synthesis and Characterization of Unmodified Ribozymes and Substrates,s can be end-labeled. Simple procedures generate a sequence ladder to confirm colinearity of template and transcript (.,.). This is not a “mere luxury,” since examples with significant template slippage have been reported (.). If a small labeled cleavage product is produced, also the exact cleavage 作者: 情感 時(shí)間: 2025-3-26 04:20 作者: adroit 時(shí)間: 2025-3-26 09:39 作者: Hypopnea 時(shí)間: 2025-3-26 13:55 作者: 陰謀 時(shí)間: 2025-3-26 20:20 作者: 即席演說(shuō) 時(shí)間: 2025-3-26 23:54 作者: entitle 時(shí)間: 2025-3-27 02:24 作者: micronutrients 時(shí)間: 2025-3-27 06:44
Minimized Hammerhead Ribozymes,form stem-loop II, and nucleotides at the 5′- and 3′-ends, which form helix I and helix III, respectively, in complex with the substrate. Such a ribozyme is shown in .. Most hammerhead ribozymes of this type are constructed with 12 nucleotides in stem-loop II, with four nucleotides in the loop and e作者: ELUC 時(shí)間: 2025-3-27 10:49 作者: Nonconformist 時(shí)間: 2025-3-27 14:19 作者: emulsify 時(shí)間: 2025-3-27 18:48 作者: Maximize 時(shí)間: 2025-3-27 23:53 作者: reject 時(shí)間: 2025-3-28 04:57 作者: 注意力集中 時(shí)間: 2025-3-28 07:09
Chemical Synthesis, Analysis, and Purification of Ribozymes,thesized RNA (.–.), equivalent to that of RNA derived by transcription methods (.–.), is contingent on efficient synthesis and purification protocols. Chemical synthesis also allows for site-specific modifications, such as deoxynucleotides, phosphorothioates and modified nucleotides (.–.).作者: eczema 時(shí)間: 2025-3-28 13:06 作者: organism 時(shí)間: 2025-3-28 16:14 作者: Rotator-Cuff 時(shí)間: 2025-3-28 19:36 作者: 侵蝕 時(shí)間: 2025-3-29 01:52
Maxine J. McCall,Philip Hendry,Trevor J. Lockettlich komplizierte Spektrum des sediment?ren organischen Materials erkennbar machen k?nnen. Die Kenntnis der Struktur und Konzentration dieser durch diagenetische Vorg?nge gebildeten Verbindungen ist nicht nur im Hinblick auf ihre m?glichen biologischen Auswirkungen wichtig, sondern auch für die Nutz作者: 換話題 時(shí)間: 2025-3-29 05:40
Qiao Yu,John M. Burkeen ansammeln k?nnen. Gr??ere Pakete von abgelagerter organischer Substanz in Form von Torfen sind zur Bildung von Kohlenlagerst?tten bef?higt. Entscheidend für die M?glichkeit einer Erd?l-, Erdgas- oder Kohlenbildung ist, neben der Ablagerung von organischem Material in ausreichender Menge, seine ma作者: Afflict 時(shí)間: 2025-3-29 09:37 作者: 旅行路線 時(shí)間: 2025-3-29 11:40 作者: 侵害 時(shí)間: 2025-3-29 16:51
Computer Analysis of the Conservation and Uniqueness of Ribozyme-Targeted HIV Sequences,ctional motif is also present in normal human mRNA, it too might be cleaved by the HIV therapeutic ribozyme, possibly resulting in harmful side effects. For these reasons, a procedure has been developed for searching the available sequence databases to determine (.) an approximate percentage of cons作者: 嫌惡 時(shí)間: 2025-3-29 22:12
Selection of Accessible Sites for Ribozymes on Large RNA Transcripts,gions identified by the random library, can then be made to hammerhead ribozyme NUX consensus target sites, where N = A, C, G, or U and X = A, C, and U (.,.). Cleavage with RNase H can be used to evaluate the binding of these antisense oligodeoxynucleotides and so to choose target sites for ribozyme作者: infinite 時(shí)間: 2025-3-30 03:25
T7 Transcript Length Determination Using Enzymatic RNA Sequencing,zation (.). This phenomenon is believed to occur when the initial transcription product can be used as either an intermolecular or intramolecular RNA primer that is subsequently extended by T7 RNA polymerase.作者: Ornithologist 時(shí)間: 2025-3-30 07:29
Design and Production of Asymmetric Hammerhead Ribozymes,ree nucleotides (.). This “design” (..) has the advantage that the catalytic antisense RNA is physically dissected into its two functional domains, which control (1) association with the target RNA and (2) cleavage of the target RNA. Such an asymmetric hammerhead ribozyme resembles an antisense RNA 作者: nonplus 時(shí)間: 2025-3-30 10:27
Book 1997er analysis may be a useful starting point to select tar- get sites, there seems, at the moment, to be no guarantee that any particular chosen site will be efficiently cleaved. Some workers have deliberately bypassed this problem by using libraries of ribozyme sequences and by select- ing those that作者: Duodenitis 時(shí)間: 2025-3-30 12:27
Zusammenfassung für Entscheidungstr?gern findet eine rasante Expansion des ?westlichen“ Lebensstils über alle Grenzen statt. Viele traditionelle Kulturen werden zurückgedr?ngt oder l?sen sich auf. Besonders betroffen sind Religionen, Kunst- oder Handwerksstile und Sprachen, letztlich aber auch alle Spielarten gesellschaftlicher Normen und Werthaltungen.作者: FLOAT 時(shí)間: 2025-3-30 17:36 作者: 細(xì)胞學(xué) 時(shí)間: 2025-3-30 21:53 作者: 得罪 時(shí)間: 2025-3-31 01:36
Cyber-Attack in ICT Cloud Computing System,tem and then lunch the attacks. We have a tendency to observe that higher corruption and an oversized Web information measure favor attacks origination. We have a tendency to additionally notice that countries with higher per-capita-GDP and higher info and communication technologies (ICT) infrastruc作者: 強(qiáng)制令 時(shí)間: 2025-3-31 07:44
e. We will also provide an extensive review of the basic principles in erection physiology and pharmacology, allowing a broader understanding of the implications of PDU in clinical practice...978-3-031-55651-7978-3-031-55649-4作者: 功多汁水 時(shí)間: 2025-3-31 11:16 作者: 保全 時(shí)間: 2025-3-31 16:39 作者: Anticoagulants 時(shí)間: 2025-3-31 21:08
strategies, mass customization stands out, for producing nearly tailored products based on customers preferences, while still using massive production techniques that allow keeping costs burdened. However, to embrace mass customization several operations at shop-floor level of the industry have to
