標(biāo)題: Titlebook: Riboregulator Design and Analysis; James Chappell,Melissa K. Takahashi Book 2022 The Editor(s) (if applicable) and The Author(s), under ex [打印本頁] 作者: Odious 時間: 2025-3-21 18:01
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Riboregulator Design and Analysis978-1-0716-2421-0Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Genome 時間: 2025-3-22 02:05
James Chappell,Melissa K. TakahashiIncludes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts作者: Expurgate 時間: 2025-3-22 06:47 作者: Obstreperous 時間: 2025-3-22 12:23 作者: homocysteine 時間: 2025-3-22 15:00 作者: COLON 時間: 2025-3-22 17:12 作者: 培養(yǎng) 時間: 2025-3-23 00:24 作者: Heart-Rate 時間: 2025-3-23 02:15 作者: 一加就噴出 時間: 2025-3-23 07:54
Design, Characterization, and Application of Targeted Gene Activation in Bacteria Using a Modular Cerest. Here, we describe a protocol for designing, characterizing, and applying the modular CRISPRa system for gene activation in .. We first focus on how to identify activating sites upstream of promoters and the cloning of the targeting sgRNA. We then describe how to perform a fluorescence experim作者: 愛了嗎 時間: 2025-3-23 13:05 作者: GLOOM 時間: 2025-3-23 15:12
Book 2022necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, andtips on troubleshooting and avoiding known pitfalls.?.Authoritative and practical, .Riboregulator Design and Analysis. is an ideal guide for scientists and engineers interested in the design and application作者: NUDGE 時間: 2025-3-23 21:35
Trevor R. Simmons,Andrew D. Ellington,Lydia M. Contrerasentionen oder Institutionen einerseits und andererseits durch materielle Beschr?nkungen (Budgetbeschr?nkung; begrenzter Zugriff auf Naturressourcen, Zeitlimit) individuelle Optimierungskalküle unter sehr einschr?nkenden Nebenbedingungen stattfinden. Wenn man in der Regel in der ?konomie annimmt, das作者: Confidential 時間: 2025-3-24 01:13 作者: 不斷的變動 時間: 2025-3-24 02:55
Seongho Hong,Dongwon Park,Soma Chaudhary,Griffin McCutcheon,Alexander A. Green,Jongmin Kimkunft aus den Lebensvorg?ngen der Organismen herleiten oder die mehr oder weniger ver?nderte überreste lebender Substanz darstellen; als Beispiele für solche Mineralien und Gesteine sind Phosphatgesteine, Kalksteine. die fossilen Harze und die Kohlen zu nennen. Nicht zu den Mineralien geh?ren die ho作者: 全等 時間: 2025-3-24 07:11 作者: Aqueous-Humor 時間: 2025-3-24 11:30 作者: intrigue 時間: 2025-3-24 17:09 作者: 群島 時間: 2025-3-24 20:45 作者: 愉快么 時間: 2025-3-24 23:19 作者: 掃興 時間: 2025-3-25 03:49 作者: Ointment 時間: 2025-3-25 08:03 作者: backdrop 時間: 2025-3-25 15:25 作者: innovation 時間: 2025-3-25 18:16
Maria Claudia Villegas Kcam,James Chappelle Chemie sich befa?t, so wird neben der Umwandlung von Materie auch die Analyse ihrer Zusammensetzung und die Beschreibung ihrer Eigenschaften (ihre Charakterisierung) zu nennen sein. Dabei behalten wir im Auge, da? Teilbereiche innerhalb dieser Gebiete von den naturwissenschaftlichen Schwesterdiszi作者: judicial 時間: 2025-3-25 22:25 作者: GRUEL 時間: 2025-3-26 01:45 作者: 多嘴 時間: 2025-3-26 07:36 作者: debase 時間: 2025-3-26 09:50
Ewelina M. Ma?ecka,Boyang Hua,Sarah A. Woodson dem Physiologen . empfohlene Jodoform, aber bei allen diesen Mitteln folgte, wie bei der Carbols?ure, einer Periode einseitiger übersch?tzung des Wertes eine skeptische Beurteilung, da die Zahl der Vergiftungen, zumal beim Jodoform, sehr groft blieb. Inzwischen war an die Stelle der antiseptischen 作者: 殘廢的火焰 時間: 2025-3-26 13:13 作者: 低三下四之人 時間: 2025-3-26 17:20 作者: Heart-Attack 時間: 2025-3-26 22:53
1064-3745 expertsThis collection explores the latest advances in riboregulators, such as RNA-only systems and ribonucleoprotein systems, and provides detailed techniques to study, evolve, and design them. Beginning with a set of chapters focused on the design and application of small RNA (sRNA) regulator sys作者: sorbitol 時間: 2025-3-27 02:56
Harnessing CRISPR-Cas9 for Epigenetic Engineering,to transiently transfecting the gRNA plasmid and the CRISPR/dCas9-based epigenetic effector and finalizing with chromatin immunoprecipitation (ChIP) to validate changes in epigenetic state at a targeted genomic region.作者: erythema 時間: 2025-3-27 08:05 作者: 愚笨 時間: 2025-3-27 13:12 作者: 蹣跚 時間: 2025-3-27 15:08
Design of RNA-Based Translational Repressors,llent orthogonality, and logic capabilities. Riboregulators that provide the inverse function – turning off translation in response to a trigger RNA – are also versatile tools for sensing and efficiently implementing logic gates such as NAND or NOR. Toehold and three-way junction (3WJ) repressors ar作者: Hamper 時間: 2025-3-27 17:59 作者: 光滑 時間: 2025-3-28 01:01 作者: Esalate 時間: 2025-3-28 02:54
Design and Assembly of Multilevel Transcriptional and Translational Regulators for Stringent Controy to stringently control gene expression due to the use of a single type of regulator that can be susceptible to unavoidable biomolecular fluctuations. In contrast, multilevel controllers (MLCs) employ several forms of regulation simultaneously to overcome this issue, ensuring a reduced basal expres作者: NAVEN 時間: 2025-3-28 08:16 作者: 強有力 時間: 2025-3-28 10:38 作者: Ovulation 時間: 2025-3-28 17:31
Development of Synthetic Riboswitches to Guide the Evolution of Metabolite Production in Microorganregulate the expression of a gene or an operon depending on the intracellular level of a target molecule and consequently modulate the cellular responses. In evolutionary engineering, riboswitch-based biosensors have been widely applied for high-throughput screening or selection of target phenotypes作者: Acetabulum 時間: 2025-3-28 21:16
Efficient Method to Identify Synthetic Riboswitches Using RNA-Based Capture-SELEX Combined with In based switches have to exhibit excellent binding affinity as well as ligand binding-induced structural changes. Selection via Capture-SELEX favors the enrichment of aptamers which exhibit both characteristics. For the Capture-SELEX, an RNA pool is used that gets immobilized onto a capture oligonucle作者: 破裂 時間: 2025-3-29 02:55
RNA Design Principles for Riboswitches that Regulate RNase P-Mediated tRNA Processing, an aptamer platform for the highly selective ligand recognition and an expression platform which controls gene activity typically at the level of transcription or translation. In previous work, we could successfully apply RNA folding prediction to implement a new riboswitch mechanism regulating pro作者: subordinate 時間: 2025-3-29 05:31
Design, Characterization, and Application of Targeted Gene Activation in Bacteria Using a Modular Cof the single guide RNA (sgRNA) to target DNA sequences. Included in this are CRISPR activation (CRISPRa) systems. These systems allow users to activate transcription of a target gene through the localization of transcription activation domains (ADs) near promoter elements, which in turn recruit RNA作者: 微生物 時間: 2025-3-29 08:33
Reprogramming TracrRNAs for Multiplexed RNA Detection,e how our recent discovery of noncanonical crRNAs inspired the engineering of reprogrammed tracrRNAs and led to a powerful platform for multiplexed RNA detection. We provide detailed protocols regarding how to design reprogrammed tracrRNA and carry out?assays in vitro and in vivo.作者: 嬰兒 時間: 2025-3-29 11:26
Harnessing CRISPR-Cas9 for Epigenetic Engineering, promoters and enhancers. Here, we describe a basic methodology for performing an epigenome editing experiment, starting from gRNA design and cloning to transiently transfecting the gRNA plasmid and the CRISPR/dCas9-based epigenetic effector and finalizing with chromatin immunoprecipitation (ChIP) t作者: 機制 時間: 2025-3-29 17:17
RNA Structure Prediction, Analysis, and Design: An Introduction to Web-Based Tools,utational algorithms that utilize thermodynamics have been developed to predict RNA secondary structure. Due to the importance of intermolecular interactions, the algorithms have been expanded to determine and predict RNA-RNA hybridization. This chapter discusses popular webservers with the tools fo作者: 匍匐前進 時間: 2025-3-29 22:19
Single-Molecule FRET Studies of RNA Structural Rearrangements and RNA-RNA Interactions, its target are typically facilitated by a protein partner. Single-molecule fluorescence microscopy is a powerful tool for dissecting the conformational states and intermediates that contribute to target recognition. This chapter describes protocols for studying target recognition by bacterial small作者: 無禮回復(fù) 時間: 2025-3-30 00:35 作者: Laconic 時間: 2025-3-30 05:43
Computational Design of Small Transcription Activating RNAs (STARs),of terminator hairpins to activate the transcription of targeted genes. STARs have found broad utility for creating synthetic gene circuits, engineering metabolic pathways, and creating new types of diagnostics. Here we describe the method to computationally design, build, and characterize STAR regulators.作者: 刪除 時間: 2025-3-30 08:44 作者: Meditative 時間: 2025-3-30 15:45 作者: intelligible 時間: 2025-3-30 17:29 作者: 闖入 時間: 2025-3-30 22:04 作者: Binge-Drinking 時間: 2025-3-31 04:23 作者: GEAR 時間: 2025-3-31 06:18
Efficient Method to Identify Synthetic Riboswitches Using RNA-Based Capture-SELEX Combined with In otide through structural changes. These sequences get reverse transcribed, PCR amplified, and used for the following selection rounds. In this publication, we present a detailed protocol for Capture-SELEX, followed by screening in yeast to identify aptamers suitable for the design of synthetic riboswitches.作者: neutralize 時間: 2025-3-31 11:41
RNA Design Principles for Riboswitches that Regulate RNase P-Mediated tRNA Processing,e biochemical analysis for the verification of the implemented mechanism. Furthermore, we discuss the applicability of the presented biochemical in vivo and in vitro methods for the characterization of other artificial riboswitches.作者: 隱藏 時間: 2025-3-31 16:33
Single-Molecule FRET Studies of RNA Structural Rearrangements and RNA-RNA Interactions,f suitable RNA substrates for sRNA-mRNA annealing reactions, preparation of internally labeled mRNA for detecting conformational changes in the target, and key steps of the data analysis. These protocols can be adapted to other RNA-binding proteins that chaperone RNA interactions.作者: 仲裁者 時間: 2025-3-31 19:18
RNP-Based Control Systems for Genetic Circuits in Synthetic Biology Beyond CRISPR,de users a streamlined method for identifying an RNP control system most useful to their own work. Lastly, we discuss future applications of RNP control systems and how they can be utilized to address the current grand challenges of the synthetic biology community.作者: 具體 時間: 2025-4-1 00:37 作者: 保守黨 時間: 2025-4-1 03:56 作者: 薄膜 時間: 2025-4-1 07:08
Trevor R. Simmons,Andrew D. Ellington,Lydia M. Contrerasnswettl?ufe, Protektionismus, ?ffentliche Verschwendung und Bürokratisierung, soziale Ungerechtigkeit, Umweltverschmutzung, Wohnungsleerstand bei gleichzeitigem Nachfrageüberhang auf dem Wohnungsmarkt und anderes mehr. Die Wirtschaftswissenschaften bieten zur Analyse der genannten Fragen eine Reihe
