標(biāo)題: Titlebook: Rheumatoid Arthritis; Methods and Protocol Shuang Liu Book 2024Latest edition The Editor(s) (if applicable) and The Author(s), under exclus [打印本頁] 作者: 宣告無效 時(shí)間: 2025-3-21 19:01
書目名稱Rheumatoid Arthritis影響因子(影響力)
書目名稱Rheumatoid Arthritis影響因子(影響力)學(xué)科排名
書目名稱Rheumatoid Arthritis網(wǎng)絡(luò)公開度
書目名稱Rheumatoid Arthritis網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Rheumatoid Arthritis被引頻次
書目名稱Rheumatoid Arthritis被引頻次學(xué)科排名
書目名稱Rheumatoid Arthritis年度引用
書目名稱Rheumatoid Arthritis年度引用學(xué)科排名
書目名稱Rheumatoid Arthritis讀者反饋
書目名稱Rheumatoid Arthritis讀者反饋學(xué)科排名
作者: 逗它小傻瓜 時(shí)間: 2025-3-21 21:03
nungsmotoren dargestellt. Mit Hilfe der diskutierten Verfahren k?nnen die grundlegenden Dimensionen und Geometrieparameter wie Durchmesser, Querschnittsverl?ufe, Radien, Formen aber auch andere Kenngr??en wie beispielsweise Nenndrehzahlen festgelegt werden, um sicherzustellen, dass die betreffende S作者: 過份艷麗 時(shí)間: 2025-3-22 02:45 作者: 網(wǎng)絡(luò)添麻煩 時(shí)間: 2025-3-22 08:24
Shuang Liu Detaillierungsgrad aber auch in ihren Rechenzeiterfordernissen unterscheiden, siehe Stiesch (2003). Als ph?nomenologische Modelle werden dabei üblicherweise die Berechnungsmodelle bezeichnet, die die Verbrennung und Schadstoffbildung in Abh?ngigkeit übergeordneter physikalischer und chemischer Ph?n作者: 任命 時(shí)間: 2025-3-22 11:17
Shuang Liunungsmotoren dargestellt. Mit Hilfe der diskutierten Verfahren k?nnen die grundlegenden Dimensionen und Geometrieparameter wie Durchmesser, Querschnittsverl?ufe, Radien, Formen aber auch andere Kenngr??en wie beispielsweise Nenndrehzahlen festgelegt werden, um sicherzustellen, dass die betreffende S作者: 生氣地 時(shí)間: 2025-3-22 15:18
Erika Takemasa,Shuang Liu Detaillierungsgrad aber auch in ihren Rechenzeiterfordernissen unterscheiden, siehe Stiesch (2003). Als ph?nomenologische Modelle werden dabei üblicherweise die Berechnungsmodelle bezeichnet, die die Verbrennung und Schadstoffbildung in Abh?ngigkeit übergeordneter physikalischer und chemischer Ph?n作者: growth-factor 時(shí)間: 2025-3-22 19:22 作者: Hemiplegia 時(shí)間: 2025-3-22 22:26 作者: 勛章 時(shí)間: 2025-3-23 03:10 作者: textile 時(shí)間: 2025-3-23 07:01 作者: Complement 時(shí)間: 2025-3-23 12:54 作者: 機(jī)械 時(shí)間: 2025-3-23 15:38 作者: 反對 時(shí)間: 2025-3-23 21:50 作者: 豎琴 時(shí)間: 2025-3-24 00:07
Erika Takemasa,Shuang Liudargestellt. Mit Hilfe der diskutierten Verfahren k?nnen die grundlegenden Dimensionen und Geometrieparameter wie Durchmesser, Querschnittsverl?ufe, Radien, Formen aber auch andere Kenngr??en wie beispielsweise Nenndrehzahlen festgelegt werden, um sicherzustellen, dass die betreffende Str?mungsmasch作者: FLEET 時(shí)間: 2025-3-24 04:17
Shuang Liu?mten K?rpers die erste fundamentale Voraussetzung zur analytischen Untersuchung und Behandlung aeroelastischer Vorg?nge. Dieses elasto-mechanische Verhalten kann in allgemeiner Form durch den in Abschnitt 1.2.2 definierten strukturellen Operator O. () zum Ausdruck gebracht werden. In diesem Kapitel作者: crease 時(shí)間: 2025-3-24 10:06
Human Xenograft Modelaneously. Same patient-derived peripheral mononuclear cell chimerism can be successfully achieved by intraperitoneal engraftment. This xenograft model is able to be used for the initial screening of human target-specified biologics.作者: 加花粗鄙人 時(shí)間: 2025-3-24 14:21
Preparation of Joint Extracts protein analysis is joint tissue extraction. Unlike other large rodents, obtaining synovium from model mice is challenging since it is so small and fragile. In this chapter, methods for harvesting synovium through a quadriceps approach and preparing protein extracts are introduced.作者: 整潔漂亮 時(shí)間: 2025-3-24 17:02
Production of Immunizing Antigen Proteoliposome Using Cell-Free Protein Synthesis Systemult. Recently, we developed a highly efficient cell-free production method of proteoliposome antigen using a cell-free protein synthesis method with liposome and dialysis cup. Here, we introduce practical and efficient integrated procedures to produce a large amount of proteoliposome antigen for anti-membrane protein antibody development.作者: uveitis 時(shí)間: 2025-3-24 20:07
Autoantibody Profiling Using Human Autoantigen Protein Array and AlphaScreen an autoantibody profiling assay using a cell-free synthesized protein array and high-throughput screening technology. Our assay system can sensitively detect interaction between recombinant antigen protein and autoantibody and efficiently analyze autoantibody profiling in patients’ sera.作者: 不可知論 時(shí)間: 2025-3-25 03:01 作者: prolate 時(shí)間: 2025-3-25 06:12 作者: SOW 時(shí)間: 2025-3-25 08:37
Denervation-Induced Sarcopenia Modele underlying mechanism of sarcopenia in RA could be assessed. Also, an examination of the efficacy of anti-rheumatic regents on sarcopenia and the influence of sarcopenia management on RA improvement is also achievable.作者: nocturia 時(shí)間: 2025-3-25 12:29
Histological Analyses of Arthritic Joints in Collagen-Induced Arthritis Model Miceng and decalcifying solution is important. In this chapter, we describe the procedures of preparing paraffin samples, including fixation, decalcification, embedding, and sectioning from the RA model mouse, as well as different staining methods (hematoxylin and eosin, tartrate-resistant acid?phosphatase).作者: 喚起 時(shí)間: 2025-3-25 16:38
1064-3745 ation advice from the experts.This second edition explores standard laboratory protocols and methodology commonly used in basic and translational studies in the field of rheumatoid arthritis (RA) treatment. Chapters detail including basic RA models, evaluation of disease activity and immunological s作者: reptile 時(shí)間: 2025-3-25 20:26
Collagen-Induced Arthritis Models erosion. In this chapter, a protocol for the successful induction of CIA in mice is described. In this protocol, CIA is induced by active immunization by inoculation with type II heterologous collagen in Freund’s adjuvant in susceptible DBA/1 mice.作者: Panacea 時(shí)間: 2025-3-26 03:28
Scaffolded Chondrogenic Spheroid-Engrafted Modelor cartilage repair can be screened on a large scale ex vivo. Furthermore, atelocollagen-scaffolded chondrogenic spheroids can be utilized for in vivo transplantation into a humanized xenografted arthritis model. Thus, the ability of cartilage self-repair can be qualitatively and quantitatively evaluated.作者: 假設(shè) 時(shí)間: 2025-3-26 08:18
Long-Term Constant Subcutaneous Drug Administrationules. Most importantly, a stable peripheral concentration of a drug can be obtained using this constant drug delivery system, which would benefit researchers in verifying the efficiency of anti-rheumatoid drugs and establishing safety profiles in preclinical studies.作者: 玩笑 時(shí)間: 2025-3-26 09:33
Production of Neutralizing Antibody cell fusion, hybridoma screening, expanding positive hybridomas, and purification, is introduced. Aiming at specific affinity to a membrane protein, synthetic proteoliposomes are used in the immunization and screening steps.作者: irreducible 時(shí)間: 2025-3-26 13:23
Collagen-Induced Arthritis Modelsfor further understanding the pathophysiology of RA and seeking potential therapeutic targets or strategies. The collagen-induced arthritis (CIA) model is one of the standard RA models used in preclinical research. The CIA model shares several pathological features with RA, such as breach of toleran作者: Insatiable 時(shí)間: 2025-3-26 20:38 作者: 多余 時(shí)間: 2025-3-26 21:46 作者: Paraplegia 時(shí)間: 2025-3-27 04:13 作者: 憤怒歷史 時(shí)間: 2025-3-27 05:18
Long-Term Constant Subcutaneous Drug Administrationic Pumps osmotic pump, continuous zero-order delivery of drugs under investigation that need repeated oral or intravenous dosing is realizable. Compared to traditional delivery systems, implanted osmotic pumps present several advantages, such as that no external connections or researcher interventio作者: ingestion 時(shí)間: 2025-3-27 10:31
Clinical Scoring of Disease Activity in Animal Modelsstological examination of joints. Clinical scoring using a qualitative scoring system of paw inflammation (paw thickness, width, or volume) over time is the standard method used for subjective quantification of arthritis activity. To evaluate paw swelling status, a quantitative method using three-di作者: 高貴領(lǐng)導(dǎo) 時(shí)間: 2025-3-27 16:13
Histological Analyses of Arthritic Joints in Collagen-Induced Arthritis Model Miceammatory disease characterized by increased synovial tissue and osteoclasts, angiogenesis, infiltration of inflammatory cells, and pannus formation. These pathologies can be observed in a collagen-induced arthritis model mouse using formaldehyde-fixated paraffin-embedded (FFPE) samples. For the prep作者: 無法取消 時(shí)間: 2025-3-27 20:46 作者: canvass 時(shí)間: 2025-3-28 01:50
Production of Immunizing Antigen Proteoliposome Using Cell-Free Protein Synthesis Systemrane proteins. However, developing antibodies against membrane proteins remains a big challenge because mass production of membrane proteins is difficult. Recently, we developed a highly efficient cell-free production method of proteoliposome antigen using a cell-free protein synthesis method with l作者: Hyperplasia 時(shí)間: 2025-3-28 04:46 作者: 彎曲道理 時(shí)間: 2025-3-28 06:37
Autoantibody Profiling Using Human Autoantigen Protein Array and AlphaScreen diseases. Analysis of autoantibodies is essential for investigating pathology mechanisms, diagnosis, and therapeutics of these diseases. We developed an autoantibody profiling assay using a cell-free synthesized protein array and high-throughput screening technology. Our assay system can sensitivel作者: 一起 時(shí)間: 2025-3-28 11:47
Generation of Specific Aptamersherapeutic molecules with high affinity and specificity and have been developed as potent drugs for the treatment of rheumatoid arthritis. Aptamers have unique and advantageous features over antibodies, such as superior affinity with nano- or pico-molar dissociation constants and ease of chemical sy作者: 心神不寧 時(shí)間: 2025-3-28 17:54 作者: vibrant 時(shí)間: 2025-3-28 20:39
RNA Interference Ex Vivoly accessed by traditional pharmacological approaches. For preclinical research on rheumatoid arthritis (RA), silencing of target genes in primary immune cells can be easily achieved by the application of small interfering RNA (siRNA) and synthetic short hairpin RNA (shRNA). Cellular and systemic ad作者: Delude 時(shí)間: 2025-3-28 23:27
Lentiviral-Mediated Systemic RNA Interference In Vivomise in its ability to efficiently transduce immune cells and hematopoietic stems cells, which are the initial therapeutic target of autoimmune diseases, and considering that gene manipulation of these cells is usually difficult to achieve using other techniques. In previous chapters, we have descri作者: Monocle 時(shí)間: 2025-3-29 05:54
Lentiviral Production Platformmodification can be achieved easily in immune cells, nondividing, and terminally differentiated cells, including hematopoietic stem cells, neurons, and even tumor cells, which other viral vectors cannot do. The main considerations of therapeutic gene delivery using a lentiviral system are the risk o作者: elucidate 時(shí)間: 2025-3-29 09:31
Book 2024Latest editiond arthritis (RA) treatment. Chapters detail including basic RA models, evaluation of disease activity and immunological status, systemic drug delivery, and new research tools. Written in the highly successful?.Methods in Molecular Biology.?series format, chapters include introductions to their respe作者: enflame 時(shí)間: 2025-3-29 13:25
Clinical Scoring of Disease Activity in Animal Modelsmensional T2-weighted flash sequence magnetic resonance imaging (MRI) is introduced. The efficacy of a therapeutic approach can be semiologically quantified using a clinical scoring system and an index of paw inflammation in CIA mice.作者: evasive 時(shí)間: 2025-3-29 16:17
Generation of Specific Aptamersnthesis, modification, and inactivation by designing antisense sequences. In this chapter, using a DNA-oligonucleotide pool, the technology of proteoliposome-systematic evolution of ligands by exponential enrichment (SELEX) is introduced. By using this technique, potential therapeutic agents with high affinity and specificity could be obtained.作者: 棲息地 時(shí)間: 2025-3-29 21:23
RNA Interference Ex Vivoministration of siRNA or shRNA has been a significant advance in preclinical research on RA. In this chapter, the basic techniques for gene silencing in human-derived peripheral T cells using liposome-dependent siRNA transfection and lentiviral-mediated shRNA delivery, aiming at gene silencing of therapeutic targets, are introduced.作者: Nonthreatening 時(shí)間: 2025-3-30 02:05 作者: follicular-unit 時(shí)間: 2025-3-30 06:28 作者: 祖?zhèn)?nbsp; 時(shí)間: 2025-3-30 10:08
rie gilt, d. h. dass die Str?mung als rein eindimensional betrachtet werden kann. Zudem wird meist angenommen, dass die Str?mung als viskosit?tsfrei betrachtet werden kann. In erster N?herung sind diese Annahmen sicher auch gerechtfertigt. Str?mungen in Str?mungsmaschinen sind jedoch im Allgemeinen 作者: 夾死提手勢 時(shí)間: 2025-3-30 16:17
Maya Miyoshi,Shuang Liurie gilt, d. h. dass die Str?mung als rein eindimensional betrachtet werden kann. Zudem wird meist angenommen, dass die Str?mung als viskosit?tsfrei betrachtet werden kann. In erster N?herung sind diese Annahmen sicher auch gerechtfertigt. Str?mungen in Str?mungsmaschinen sind jedoch im Allgemeinen 作者: lymphoma 時(shí)間: 2025-3-30 16:47
Shuang Liurennraum zu jedem Zeitpunkt vereinfachend als ideal durchmischt annehmen und die auf empirischen Ans?tzen für die Brennrate beruhen. Auf der anderen Seite unterscheiden sich die ph?nomenologischen Verbrennungsmodelle von den CFD-Codes (CFD = computational fluid dynamics, vgl. Kap. 12 ff.), indem auf作者: 脫落 時(shí)間: 2025-3-30 23:55
Shuang Liurie gilt, d. h. dass die Str?mung als rein eindimensional betrachtet werden kann. Zudem wird meist angenommen, dass die Str?mung als viskosit?tsfrei betrachtet werden kann. In erster N?herung sind diese Annahmen sicher auch gerechtfertigt. Str?mungen in Str?mungsmaschinen sind jedoch im Allgemeinen 作者: Loathe 時(shí)間: 2025-3-31 03:44
Erika Takemasa,Shuang Liurennraum zu jedem Zeitpunkt vereinfachend als ideal durchmischt annehmen und die auf empirischen Ans?tzen für die Brennrate beruhen. Auf der anderen Seite unterscheiden sich die ph?nomenologischen Verbrennungsmodelle von den CFD-Codes (CFD = computational fluid dynamics, vgl. Kap. 12 ff.), indem auf作者: 減震 時(shí)間: 2025-3-31 06:07 作者: 情愛 時(shí)間: 2025-3-31 12:26 作者: BROW 時(shí)間: 2025-3-31 15:45
