標題: Titlebook: Reverse Genetics of RNA Viruses; Methods and Protocol Daniel R. Perez Book 2017 Springer Science+Business Media LLC 2017 reverse genetics.R [打印本頁] 作者: fathom 時間: 2025-3-21 18:20
書目名稱Reverse Genetics of RNA Viruses影響因子(影響力)
書目名稱Reverse Genetics of RNA Viruses影響因子(影響力)學科排名
書目名稱Reverse Genetics of RNA Viruses網(wǎng)絡公開度
書目名稱Reverse Genetics of RNA Viruses網(wǎng)絡公開度學科排名
書目名稱Reverse Genetics of RNA Viruses被引頻次
書目名稱Reverse Genetics of RNA Viruses被引頻次學科排名
書目名稱Reverse Genetics of RNA Viruses年度引用
書目名稱Reverse Genetics of RNA Viruses年度引用學科排名
書目名稱Reverse Genetics of RNA Viruses讀者反饋
書目名稱Reverse Genetics of RNA Viruses讀者反饋學科排名
作者: 維持 時間: 2025-3-21 23:44
Book 2017ary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Authoritative and practical, .Reverse Genetics of RNA Viruses: Methods and Protocols .aims to ensure successful results in the further study of this vital field..作者: 軟弱 時間: 2025-3-22 02:47
Reverse Genetics for Mammalian Orthoreovirus,cs approaches have contributed immeasurably to our understanding of viral replication and pathogenesis, and also have led to development of novel vaccines and virus-based vectors. Here, we describe the reverse genetics system that allows for production and recovery of mammalian orthoreovirus, a doub作者: Indent 時間: 2025-3-22 05:14
Development and Characterization of an Infectious cDNA Clone of Equine Arteritis Virus,ribe the assembly of the full-length infectious cDNA clone of the virulent Bucyrus strain (VBS; ATCC VR-796) of EAV in a plasmid vector. This system allows generation of infectious in vitro-transcribed (IVT) RNA from the linearized plasmid that can be transfected or electroporated into mammalian cel作者: cumulative 時間: 2025-3-22 09:05 作者: dictator 時間: 2025-3-22 14:37
Reverse Genetics of Zika Virus,ne of ZIKV genome. To ensure the stability of the cDNA clone, we used a low copy vector (pACYC177) and a set of unique restriction enzyme sites on the ZIKV genome to assemble the full-length cDNA clone. A T7 promoter was engineered in front of the viral 5′ UTR for in vitro transcription. A hepatitis作者: aqueduct 時間: 2025-3-22 17:52
Efficient Reverse Genetic Systems for Rapid Genetic Manipulation of Emergent and Preemergent Infectto generate, grow, and genetically manipulate infectious CoVs in order to rapidly evaluate pathogenic mechanisms, host and tissue permissibility, and candidate antiviral therapeutic efficacy. CoVs encode the largest viral RNA genomes at about 28–32,000 nucleotides in length, and thereby complicate e作者: 樂器演奏者 時間: 2025-3-22 21:50
Reverse Genetics System for the Avian Coronavirus Infectious Bronchitis Virus,he IBV genome is inserted into the vaccinia virus genome under the control of a T7 promoter sequence. Vaccinia virus as a vector for the full-length IBV cDNA has the advantage that modifications can be introduced into the IBV cDNA using homologous recombination, a method frequently used to insert an作者: Cholagogue 時間: 2025-3-23 05:01
Rescue of Sendai Virus from Cloned cDNA, ., SeV has been characterized well, and these studies revealed numerous traits of paramyxovirus biology. The reverse genetics system to rescue SeV was first established in 1995. The virus was rescued from a cloned cDNA that contains full genome sequence flanked by T7 promoter and hepatitis delta vi作者: 雄辯 時間: 2025-3-23 06:26 作者: FAZE 時間: 2025-3-23 13:09
Recovery of a Paramyxovirus, the Human Metapneumovirus, from Cloned cDNA,arily in young children and persons with underlying disease. Based on genetic and antigenic variation, HMPV strains are classified into two serotypes, with isolates NL/1/00 and NL/1/99 as prototypes for serotypes A and B, respectively. The development of plasmid-based reverse genetics systems for bo作者: 雕鏤 時間: 2025-3-23 15:27 作者: 浪蕩子 時間: 2025-3-23 18:03 作者: 有常識 時間: 2025-3-24 02:11 作者: Projection 時間: 2025-3-24 05:45 作者: 我沒有強迫 時間: 2025-3-24 10:07
Reverse Genetics of Influenza B Viruses,ombinant influenza A viruses, plasmid-based reverse genetics techniques have allowed researchers the generation of wild type and mutant viruses from full-length cDNA copies of the influenza viral genome. These reverse genetics approaches have allowed researchers to answer important questions on the 作者: Fraudulent 時間: 2025-3-24 12:02 作者: gastritis 時間: 2025-3-24 18:20
Plasmid-Based Reverse Genetics of Influenza A Virus,merge with the potential to establish stable lineages in other avian and mammalian species, including humans. Understanding the molecular changes that allow influenza A viruses to change host range is essential to better assess their animal and public health risks. Reverse genetics systems have tran作者: 六邊形 時間: 2025-3-24 22:04
Reverse Genetics for Porcine Reproductive and Respiratory Syndrome Virus,ne expression, vaccine development, virulence determinants, and viral pathogenesis. Herein, we describe the modification of the pFL12 “RNA launch” reverse genetic system to the CMV promoter-driven pXJ41-FL13 “DNA launch” system. The generation of progeny PRRSV using pXJ41-FL13 is further elucidated.作者: 美色花錢 時間: 2025-3-25 02:55 作者: Individual 時間: 2025-3-25 06:04 作者: 粗糙 時間: 2025-3-25 09:41 作者: 強行引入 時間: 2025-3-25 12:40
Reverse Genetics System for the Avian Coronavirus Infectious Bronchitis Virus, genes. Infectious recombinant IBVs are generated in situ following the transfection of vaccinia virus DNA, containing the modified IBV cDNA, into cells infected with a recombinant fowlpox virus expressing T7 DNA-dependant RNA polymerase.作者: Exuberance 時間: 2025-3-25 18:07
Plasmid-Based Reverse Genetics of Influenza A Virus, of alternative vaccines and vaccination strategies. In this chapter we describe the cloning of cDNA copies of viral RNA segments derived from a type A influenza virus into reverse genetics plasmid vectors and the experimental procedures for the successful generation of recombinant influenza A viruses.作者: TAG 時間: 2025-3-25 20:55
Reverse Genetics for Mammalian Orthoreovirus,ines and virus-based vectors. Here, we describe the reverse genetics system that allows for production and recovery of mammalian orthoreovirus, a double-stranded (ds) RNA virus, from plasmids that encode the viral genome.作者: 舊病復發(fā) 時間: 2025-3-26 01:14 作者: debase 時間: 2025-3-26 04:36
BAC-Based Recovery of Recombinant Respiratory Syncytial Virus (RSV),sk populations. Current efforts to develop vaccines include development of live-attenuated RSV candidates. We describe here methods for preparation and recovery of recombinant RSV using an efficient bacterial artificial chromosome (BAC)-based system, expansion and plaque purification of recovered virus, and generation of master and working stocks.作者: 喪失 時間: 2025-3-26 10:10
Book 2017des readers through comprehensive protocols on RNA viruses, that were the most challenging to obtain and/or that were developed most recently. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necess作者: Highbrow 時間: 2025-3-26 12:45
1064-3745 ation advice from the experts.Includes supplementary materia.This volume is a compilation of sixteen chapters that detail reverse genetics protocols. .Reverse Genetics of RNA Viruses: Methods and Protocols. guides readers through comprehensive protocols on RNA viruses, that were the most challenging作者: helper-T-cells 時間: 2025-3-26 20:45 作者: licence 時間: 2025-3-26 22:39
B. G. van den Hoogen,R. A. M. Fouchier is useful in part by using an XML-based high-level protocol (SOAP) for message exchanges between clients and services. As long as the client can produce messages in the right format, it doesn‘t matter what lan978-1-59059-131-4978-1-4302-0818-1作者: Fraudulent 時間: 2025-3-27 03:09 作者: Ledger 時間: 2025-3-27 08:53
Adam S. Cockrell,Anne Beall,Boyd Yount,Ralph Baricnehmen bei der Umsetzung von Karriere-Websites, propagiert bereits seitJahren die Rolle von SEO im Kontext Recruiting und beobachtet Googles Aktivit?ten im Jobmarkt. Er ist Autor des Werks ?Karriere-Websites mit Wow!-Effekt und bloggt auf einem einflussreichen HR-Blog...作者: periodontitis 時間: 2025-3-27 10:26 作者: 帶子 時間: 2025-3-27 15:50
Shringkhala Bajimaya,Tsuyoshi Hayashi,Toru Takimotos, has been promoting the role of SEO in the context of recruiting for years and observes Google‘s activities in the job market. He is author of the book "Career Websites with Wow! effect" and blogs on an influential HR blog.. .This?.Springer essential.?is a translation of the original German 1st ed作者: badinage 時間: 2025-3-27 21:11 作者: Scleroderma 時間: 2025-3-28 01:39
Stivalis Cardenas-Garcia,Claudio L. Afonsoistant vision; but it still has problems. Interoperability has been achieved code creation is useful in part by using an XML-based high-level protocol (SOAP) for message exchanges between clients and services. As long as the client can produce messages in the right format, it doesn‘t matter what lan作者: INTER 時間: 2025-3-28 05:12 作者: Abutment 時間: 2025-3-28 09:38
Tobias Nolden,Stefan Finkemooted in academic circles in the 1970s, but was not allowed to appear in print because it sounded too close to bourgeois notions of rule of law (in the Anglo-American variant) or of . (in the German variant) (V. Savitsky, Institute of State and Law, Moscow, in private discussion). Now the idea has 作者: nurture 時間: 2025-3-28 12:39 作者: Conflagration 時間: 2025-3-28 17:53
Aitor Nogales,Daniel R. Perez,Jefferson Santos,Courtney Finch,Luis Martínez-Sobridoten unstable states and the dissolution of the CPSU. Perhaps this was inevitable. It is hard to see how Gorbachev could have achieved his dream of a reformed, modernized and dynamic USSR still within the grip of the Party and Marxism-Leninism. In many ways, . fits neatly into centuries-old patterns 作者: macabre 時間: 2025-3-28 19:23
Daniela Toro-Ascuy,Marcelo Cortez-San Martínot mind changing things, even drastically, as long as they were permitted to keep their positions of influence; they did not want reform to be used against them. Gorbachev stood between these two phalanxes in the fall of 1987, manoeuvring toward the more traditional positions that seemed strong sinc作者: GLEAN 時間: 2025-3-29 00:46
Daniel R. Perez,Matthew Angel,Ana Silvia Gonzalez-Reiche,Jefferson Santos,Adebimpe Obadan,Luis Marti 1987 among the proponents of . had not provided many insights, and had in fact even withered in the heat of counterattack by the Stalinist academic ideologues; second, the defence of orthodoxy tended to encourage his opponents who had brought about a tense standoff in the factional struggle in the 作者: 鄙視讀作 時間: 2025-3-29 06:09 作者: Manifest 時間: 2025-3-29 11:06
Johnasha D. Stuart,Matthew B. Phillips,Karl W. Boehme作者: Eclampsia 時間: 2025-3-29 12:44 作者: DUCE 時間: 2025-3-29 16:31
Efficient Reverse Genetic Systems for Rapid Genetic Manipulation of Emergent and Preemergent Infectgenome), each subcloned into a plasmid for increased stability and ease of genetic manipulation and amplification. Coronavirus genomes are conveniently partitioned by introducing type IIS or IIG restriction enzyme recognition sites that confer directional cloning. Since each restriction site leaves 作者: 空中 時間: 2025-3-29 21:28
Rescue of Sendai Virus from Cloned cDNA,the genome, and produces progeny virions. Rescued SeV needs to be plaque purified to exclude vTF7.3 from viral stock. Reverse genetics system of SeV is relatively efficient compared to other paramyxoviruses, but alternative approaches to rescue poorly growing mutant viruses are also available.作者: Preserve 時間: 2025-3-30 01:53
Rapid Reverse Genetics Systems for Rhabdoviruses: From Forward to Reverse and Back Again,tions into reverse genetics vector plasmids, we developed a system in which cDNA copies of complete rhabdovirus populations are inserted into a plasmid bank by linear-to-linear homologous RecE/T recombination (LLHR). Limited requirements for sequence information a priori, high cloning efficiencies, 作者: bacteria 時間: 2025-3-30 07:09
Lassa Virus Reverse Genetics,cloned cDNAs containing predetermined mutations in their genomes to investigate virus-host interactions and mechanisms of pathogenesis, as well as to facilitate screens to identify antiviral drugs against LASV and the implementation of novel strategies to develop live-attenuated vaccines (LAV). In t作者: 貞潔 時間: 2025-3-30 09:52
Reverse Genetics of Influenza B Viruses,t factors involved in influenza pathogenesis, transmissibility, host-range interactions and restrictions, and virulence. Likewise, reverse genetics techniques have been used for the implementation of inactivated or live-attenuated influenza vaccines and the identification of anti-influenza drugs and作者: Gratulate 時間: 2025-3-30 15:26
Chao Shan,Xuping Xie,Pei-Yong Shiur day-to-day life. However, Google has repeatedly been accused of acting outside the law in the development of services such as Adwords, Googlebooks or YouTube. One of the main purposes of this book is to assess whether those accusations are well-founded. But more important than that, this book pro作者: 陳腐思想 時間: 2025-3-30 16:35
Adam S. Cockrell,Anne Beall,Boyd Yount,Ralph Barics beste Sucherlebnis zu bieten, zeigt sich dank Google for Jobs nun auch in der Stellensuche. Henner Knabenreich stellt die wichtigsten Erkenntnisse zu Google for Jobs dar und vermittelt wichtige Tipps und Hinweise für die erfolgreiche Nutzung. Er zeigt, dass Unternehmen von einer nie da gewesenen M作者: Small-Intestine 時間: 2025-3-30 23:01
Erica Bickerton,Sarah M. Keep,Paul Brittons beste Sucherlebnis zu bieten, zeigt sich dank Google for Jobs nun auch in der Stellensuche. Henner Knabenreich stellt die wichtigsten Erkenntnisse zu Google for Jobs dar und vermittelt wichtige Tipps und Hinweise für die erfolgreiche Nutzung. Er zeigt, dass Unternehmen von einer nie da gewesenen M作者: zonules 時間: 2025-3-31 03:29 作者: 是剝皮 時間: 2025-3-31 06:47
Christopher C. Stobart,Anne L. Hotard,Jia Meng,Martin L. Mooregle‘s claim to offer users the best search experience is now also evident in the job search. Henner Knabenreich presents the most important findings about Google for Jobs and provides important tips and hints for successful use. He shows that companies benefit from an unprecedented opportunity to pr作者: 會犯錯誤 時間: 2025-3-31 11:14
B. G. van den Hoogen,R. A. M. Fouchierser Javascript, Java (including JSP) and .NET in a hands-on What Is This Book About? This is a book about Web Services. Web Services are still more like a movement than a mature technology. The movement is motivated by a vision of a semi-auto- mated Web that can support long chains of interactions b作者: 雪白 時間: 2025-3-31 15:23
Stivalis Cardenas-Garcia,Claudio L. Afonsovated by a vision of a semi-auto- mated Web that can support long chains of interactions between autonomous agents. There are three important components to that vision. One is interoperabil- ity: a service can have clients (agents) from any platform, in any language. Another is autonomy: an agent ca作者: buoyant 時間: 2025-3-31 19:50 作者: Orgasm 時間: 2025-4-1 01:01 作者: AFFIX 時間: 2025-4-1 03:15
Luis Martínez-Sobrido,Slobodan Paessler,Juan Carlos de la Torreof this ‘winter alliance’ saw a marked swing back towards the use of threat and violence in a vain attempt to restore the powers of the centre. Gorbachev never really had the heart and stomach to be a dictator, though. Massive protests in Moscow in March 1991 catalysed his doubts about his new allie
