標題: Titlebook: Reporter Gene Assays; Methods and Protocol Robert Damoiseaux,Samuel Hasson Book 2018 Springer Science+Business Media, LLC, part of Springer [打印本頁] 作者: PEL 時間: 2025-3-21 17:31
書目名稱Reporter Gene Assays影響因子(影響力)
書目名稱Reporter Gene Assays影響因子(影響力)學科排名
書目名稱Reporter Gene Assays網(wǎng)絡(luò)公開度
書目名稱Reporter Gene Assays網(wǎng)絡(luò)公開度學科排名
書目名稱Reporter Gene Assays被引頻次
書目名稱Reporter Gene Assays被引頻次學科排名
書目名稱Reporter Gene Assays年度引用
書目名稱Reporter Gene Assays年度引用學科排名
書目名稱Reporter Gene Assays讀者反饋
書目名稱Reporter Gene Assays讀者反饋學科排名
作者: Seizure 時間: 2025-3-21 22:22 作者: 微不足道 時間: 2025-3-22 03:44
Methods in Molecular Biologyhttp://image.papertrans.cn/r/image/827355.jpg作者: 虛假 時間: 2025-3-22 04:33 作者: Ondines-curse 時間: 2025-3-22 08:49 作者: 全能 時間: 2025-3-22 14:27
High-Throughput Firefly Luciferase Reporter Assays, identify chemical compounds capable of interfering with cellular signaling. Here, we present methods to prepare affordable firefly luciferase assay reagents and procedures to use these reagents in reporter gene high-throughput screening with large batches of 96-well cell culture plates.作者: 胰臟 時間: 2025-3-22 20:20
Using the 2A Protein Coexpression System: Multicistronic 2A Vectors Expressing Gene(s) of Interest llowed by a simple example of a “how to” protocol to concatenate multiple genes of interest, together with a reporter gene, into a single gene linked . 2As for easy identification or selection of transduced cells.作者: 過剩 時間: 2025-3-23 00:06 作者: Condense 時間: 2025-3-23 02:53 作者: 逢迎春日 時間: 2025-3-23 06:53
Gene Expression Analysis by Arylsulfatase Assays in the Green Alga ,,, we describe protocols for arylsulfatase activity assays using ., activity of which can be measured either quantitatively or qualitatively, and in low (individual sample) or high (96-well format) throughput.作者: ENNUI 時間: 2025-3-23 12:10
1064-3745 ation advice from the expertsThis volume explores the applications of reporter gene technology and the methodologies needed for their effective implementation. The chapters in this book cover practical topics such as how to integrate reporter constructs into cellular models, viral delivery, splicing作者: 讓空氣進入 時間: 2025-3-23 16:41
Reporter Gene Assays Using Transfectable Functional Genomics Libraries,f the principles for the generation, screening and data analysis of such arrayed libraries. Specifically we cover the differences between the various transfectable reagents, library selection and handling, and data analysis to offer a comprehensive understanding of these important technologies and how to apply them.作者: 財產(chǎn) 時間: 2025-3-23 21:10
Reporter Gene Assays Using Viral Functional Genomics Libraries,allenging than the transient transfection approach due to the viral production step, and the infrastructure necessary to generate them. The following pages will delve into the details for working with arrayed well formats for both lentiviral and retroviral libraries.作者: 易于出錯 時間: 2025-3-23 23:05
High-Content Reporter Assays, the screener to utilize fluorescent reporters to assess the activation state of such a high-content reporter on a cell by cell level, thus minimizing artifacts. Here we discuss the use of such a high-content reporter that enables screening for compounds useful for HIV reactivation on Jurkat cells with high-content screening.作者: 偽書 時間: 2025-3-24 06:13 作者: Corral 時間: 2025-3-24 08:08 作者: medieval 時間: 2025-3-24 12:49 作者: debunk 時間: 2025-3-24 16:45
Intravital Imaging of Human Melanoma Cells in the Mouse Ear Skin by Two-Photon Excitation Microscop Imaging fluorescence reporter gene expression in small living animals by 2PE provides a unique tool to investigate critical pathways and molecular events in cancer biology such as tumorigenesis and metastasis in vivo with high-spatial and temporal resolutions.作者: nitric-oxide 時間: 2025-3-24 21:07
Genome-Edited Cell Lines for High-Throughput Screening,ers resulting from disease-associated copy number variations, but also for induction/repression of genes modulating the severity of a disease phenotype. Traditional methods have employed transient or stable transfection of reporter constructs in which a single reporter is driven by selected regulato作者: 吹牛大王 時間: 2025-3-25 03:06 作者: GRE 時間: 2025-3-25 07:11 作者: Plaque 時間: 2025-3-25 10:02
Developing Mammalian Cellular Clock Models Using Firefly Luciferase Reporter,tissue types share a common molecular mechanism at the cellular and molecular level which underlies the rhythmic expression of genes. Individual cells are the functional units for rhythm generation and cell-based clock models offer experimental tractability for discovery. Cellular clock models can b作者: 增強 時間: 2025-3-25 14:08 作者: Melanoma 時間: 2025-3-25 18:27
High-Throughput Screening of a Luciferase Reporter of Gene Silencing on the Inactive X Chromosome, to a screening strategy for identifying factors that reactivate epigenetically silenced genes. This epigenetic luciferase reporter is subject to endogenous gene silencing mechanisms on the inactive X chromosome (Xi) in primary mouse cells and thus captures the multilayered nature of chromatin silen作者: Infect 時間: 2025-3-25 20:25
Making It All Work: Functional Genomics and Reporter Gene Assays,aling cascades, find new drug targets, or decipher the function of gene expression. The genome-wide scale of these experiments necessitates a different approach toward science than traditional single hypothesis driven research. High-throughput experimentation requires large project teams, automation作者: Agility 時間: 2025-3-26 00:58
Reporter Gene Assays Using Transfectable Functional Genomics Libraries,ies offer insight into fundamental cellular processes governing health and disease and can be utilized in an arrayed fashion which makes them uniquely suited to deconvolute complicated disease phenotypes and dissect biological networks that would otherwise be inaccessible. Here we give an overview o作者: bisphosphonate 時間: 2025-3-26 06:24
Reporter Gene Assays Using Viral Functional Genomics Libraries,ect cell lines and primary cells. One solution to this problem is?the use of virus to introduce genomic reagents. This strategy?is more commonplace now than ever before with libraries covering cDNAs, shDNAs, miRNAs, and guide RNAs readily available. Maintenance and use of these libraries are more ch作者: 認為 時間: 2025-3-26 11:15
Using YFP as a Reporter of Gene Expression in the Green Alga ,,ith promoter-fusion constructs in . is a powerful method for connecting metal-responsive regulation with .-regulatory elements, but overcoming expression-level variability between transformants and optimizing experimental conditions can be laborious. Here, we provide detailed protocols for the high-作者: venous-leak 時間: 2025-3-26 16:26 作者: 吞噬 時間: 2025-3-26 20:35 作者: 好忠告人 時間: 2025-3-26 23:01
High-Content Reporter Assays,, fluorescent reporters do not. They suffer from artifacts such as compound fluorescence and cellular debris which makes the assessment of whole well fluorescence signals difficult. However, the use of high-content screening allows for the isolation of individual cells, segmentation and thus enables作者: 連詞 時間: 2025-3-27 02:40
Data Mining and Computational Modeling of High-Throughput Screening Datasets,y datasets entering public and open databases such as ChEMBL and PubChem. The growth of academic HTS screening centers and the increasing move to academia for early stage drug discovery suggests a great need for the informatics tools and methods to mine such data and learn from it. Collaborative Dru作者: Morose 時間: 2025-3-27 07:08
Intravital Imaging of Human Melanoma Cells in the Mouse Ear Skin by Two-Photon Excitation Microscoptidimensional imaging down to single-cell detection sensitivity in vivo in deep tissues. Here we used 2PE microscopy to visualize green fluorescent protein (GFP) as a reporter gene in human melanoma cells implanted into the dermis of the mouse ear skin. We first provide a step-by-step methodology to作者: 喃喃而言 時間: 2025-3-27 10:16
Patricia Dranchak,John J. Moran,Ryan MacArthur,Camila Lopez-Anido,James Inglese,John Svaren作者: 充氣女 時間: 2025-3-27 14:33 作者: ASSET 時間: 2025-3-27 20:17 作者: Incorruptible 時間: 2025-3-28 00:45
Nathan Y. Bentolila,Raymond L. Barnhill,Claire Lugassy,Laurent A. Bentolila作者: 無意 時間: 2025-3-28 04:10
Ellen Siebring-van Olst,Victor W. van Beusechem Musiktheater besteht insofern eine Zweiteilung zwischen den ?mittelbaren“ Interpreten, die als Führungskr?fte die künstlerische Konzeption für die jeweilige Produktion entwickeln, und den ?unmittelbaren“ Interpreten, die als geführte künstlerische Mitwirkende diese Konzeption umsetzen (Dechant 1985作者: CAMP 時間: 2025-3-28 09:58 作者: perimenopause 時間: 2025-3-28 12:24 作者: Intentional 時間: 2025-3-28 16:09 作者: STEER 時間: 2025-3-28 18:45
Book 2018laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Cutting-edge and thorough, .Reporter Gene Assays: Methods and Protocols. is a valuable resource for anyone who is interestedin learning more about reporter genes..作者: 低三下四之人 時間: 2025-3-29 00:30 作者: 叫喊 時間: 2025-3-29 04:50 作者: 帶子 時間: 2025-3-29 09:39
Developing Mammalian Cellular Clock Models Using Firefly Luciferase Reporter,rcadian parameters including period length, amplitude, damping rate, and phase. Miniaturization of the assays improves throughput for large scale screens. In our lab, we have expertise for constructing circadian reporters and developing reporter cell lines. Here, we describe the procedure for establ作者: Thyroiditis 時間: 2025-3-29 11:58
High-Throughput Screening Method to Identify Alternative Splicing Regulators, response to exon inclusion and exclusion but oppositely. The method significantly eliminates false positives and allows for sensitive identification of true regulators of splicing. The method described here is designed to screen cDNA libraries, but can be applied to isolate splicing regulators from作者: multiply 時間: 2025-3-29 17:17
Data Mining and Computational Modeling of High-Throughput Screening Datasets,es researchers to share models, share predictions from models, and create models from distributed, heterogeneous data. Our system is built on top of the Collaborative Drug Discovery Vault Activity and Registration data repository ecosystem which allows users to manipulate and visualize thousands of 作者: Musket 時間: 2025-3-29 21:58 作者: 杠桿支點 時間: 2025-3-30 03:49
Garry A. Luke,Martin D. Ryan des Musiktheaters ist als weitgehend direktiv zu bezeichnen. Dies betrifft speziell den Führungsstil der künstlerischen Bühnenvorst?nde wie Dirigent, Regisseur, Chordirektor und Ballettdirektor gegenüber den künstlerischen Mitwirkenden wie Orchestermusiker, Chors?nger und T?nzer. Künstlerische Führ作者: hemorrhage 時間: 2025-3-30 07:04 作者: 接合 時間: 2025-3-30 10:37 作者: Aggressive 時間: 2025-3-30 15:34
https://doi.org/10.1007/978-3-658-43557-8uf ihrem Weg zum Meer mehrere der Ost-West angeordneten Klimagürtel. ?hnlich den überregionalen Windsystemen bilden sie bedeutende festl?ndische exogene Systeme, die unterschiedliche Klimareiche miteinander verbinden.作者: Ardent 時間: 2025-3-30 18:06 作者: ANNUL 時間: 2025-3-31 00:23 作者: Absenteeism 時間: 2025-3-31 00:54 作者: deficiency 時間: 2025-3-31 09:03 作者: 投射 時間: 2025-3-31 11:38
Book 2022ung von Lehrpersonen. Im Fokus der Untersuchung stehen dabei vor allem die Kompetenzen von Lehrerinnen und Lehrern im Zusammenhang mit der Diagnose und F?rderung von Kindern mit besonderen Schwierigkeiten beim Rechnenlernen. Um diese Kompetenzen quantitativ und handlungsnah zu erfassen, wird ein auf作者: Triglyceride 時間: 2025-3-31 16:35
