標(biāo)題: Titlebook: Regulatory Non-Coding RNAs; Methods and Protocol Gordon G. Carmichael Book 2015 Springer Science+Business Media New York 2015 Fluorescence [打印本頁(yè)] 作者: 他剪短 時(shí)間: 2025-3-21 17:23
書目名稱Regulatory Non-Coding RNAs影響因子(影響力)
作者: intangibility 時(shí)間: 2025-3-21 22:41
https://doi.org/10.1007/978-1-4939-1369-5Fluorescence in situ hybridization; Long non-coding RNAs; MicroRNA targets; Non-coding transcriptome; Pr作者: 高爾夫 時(shí)間: 2025-3-22 01:26 作者: Fatten 時(shí)間: 2025-3-22 06:28
A Biochemical Approach to Identify Direct MicroRNA Targets,y the tumor suppressor miRNA miR-34a. This method involves transfection of cells with biotinylated miRNA mimics, streptavidin pulldown, RNA isolation, and qRT-PCR. The protocol in this chapter describes these steps and the issues that should be considered while designing such pulldown experiments.作者: HALO 時(shí)間: 2025-3-22 11:13 作者: minion 時(shí)間: 2025-3-22 16:08
RNA Fluorescence In Situ Hybridization in Cultured Mammalian Cells, study of the distribution and function of lncRNAs is RNA fluorescence in situ hybridization (RNA-FISH). The protocols presented in this chapter describe this method in detail and also mention a number of critical points that must be considered when performing this technique.作者: mutineer 時(shí)間: 2025-3-22 18:14
Rapid and Efficient Elimination of Specific Nuclear Noncoding RNAs in Mammalian Cells with Antisensons have yet to be elucidated. Here, we describe a method for the rapid and efficient elimination of specific nuclear noncoding RNAs in mammalian cells using chemically modified antisense oligonucleotides, which may facilitate the functional assessment of nuclear ncRNAs.作者: 新星 時(shí)間: 2025-3-22 21:17 作者: 流動(dòng)才波動(dòng) 時(shí)間: 2025-3-23 02:13 作者: 正常 時(shí)間: 2025-3-23 08:51
Regulatory Non-Coding RNAs978-1-4939-1369-5Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 收藏品 時(shí)間: 2025-3-23 12:19 作者: Multiple 時(shí)間: 2025-3-23 15:23 作者: ATOPY 時(shí)間: 2025-3-23 19:01 作者: reflection 時(shí)間: 2025-3-23 22:52 作者: Prosaic 時(shí)間: 2025-3-24 03:46
Xuhang Liu,Zissimos Mourelatosether work carried out over four years by scientists from 25 countries working in the fields of forestry, health, environment and social sciences. While the focus is primarily on health priorities defined within Europe, this volume explicitly draws also on research from North America.978-94-007-9005-6978-90-481-9806-1作者: 引起痛苦 時(shí)間: 2025-3-24 10:04 作者: 玉米 時(shí)間: 2025-3-24 13:07
Qing-Fei Yin,Ling-Ling Chen Ph.D.,Li Yang Ph.D.ether work carried out over four years by scientists from 25 countries working in the fields of forestry, health, environment and social sciences. While the focus is primarily on health priorities defined within Europe, this volume explicitly draws also on research from North America.978-94-007-9005-6978-90-481-9806-1作者: 裝勇敢地做 時(shí)間: 2025-3-24 17:47 作者: abolish 時(shí)間: 2025-3-24 22:42
Oskar Marín-Béjar,Maite Huarteether work carried out over four years by scientists from 25 countries working in the fields of forestry, health, environment and social sciences. While the focus is primarily on health priorities defined within Europe, this volume explicitly draws also on research from North America.978-94-007-9005-6978-90-481-9806-1作者: cravat 時(shí)間: 2025-3-25 02:31 作者: Ingratiate 時(shí)間: 2025-3-25 04:19
Shinichi Nakagawaether work carried out over four years by scientists from 25 countries working in the fields of forestry, health, environment and social sciences. While the focus is primarily on health priorities defined within Europe, this volume explicitly draws also on research from North America.978-94-007-9005-6978-90-481-9806-1作者: GILD 時(shí)間: 2025-3-25 10:50 作者: Overthrow 時(shí)間: 2025-3-25 14:50
John R. Sinnamon,Kevin Czaplinskiether work carried out over four years by scientists from 25 countries working in the fields of forestry, health, environment and social sciences. While the focus is primarily on health priorities defined within Europe, this volume explicitly draws also on research from North America.978-94-007-9005-6978-90-481-9806-1作者: 上漲 時(shí)間: 2025-3-25 17:50
Tetsuro Hirose,Taro Mannenether work carried out over four years by scientists from 25 countries working in the fields of forestry, health, environment and social sciences. While the focus is primarily on health priorities defined within Europe, this volume explicitly draws also on research from North America.978-94-007-9005-6978-90-481-9806-1作者: 可能性 時(shí)間: 2025-3-25 21:34
Neena Groverether work carried out over four years by scientists from 25 countries working in the fields of forestry, health, environment and social sciences. While the focus is primarily on health priorities defined within Europe, this volume explicitly draws also on research from North America.978-94-007-9005-6978-90-481-9806-1作者: Sad570 時(shí)間: 2025-3-26 00:41
James A. Goodrich,Jennifer F. Kugelether work carried out over four years by scientists from 25 countries working in the fields of forestry, health, environment and social sciences. While the focus is primarily on health priorities defined within Europe, this volume explicitly draws also on research from North America.978-94-007-9005-6978-90-481-9806-1作者: prick-test 時(shí)間: 2025-3-26 08:05 作者: 粘土 時(shí)間: 2025-3-26 10:26 作者: 愉快么 時(shí)間: 2025-3-26 14:23 作者: Flustered 時(shí)間: 2025-3-26 18:09 作者: hallow 時(shí)間: 2025-3-26 21:17 作者: FANG 時(shí)間: 2025-3-27 05:06
Locating RNAs In Situ with FISH-STIC Probes,e a straightforward means for laboratories to design their own FISH probes that can be synthesized commercially. Here we provide a detailed protocol for applying FISH-STIC probes for in situ hybridization on cultured cells as a convenient and flexible method for localizing individual RNAs with many fluorophores using fluorescence microscopy.作者: Density 時(shí)間: 2025-3-27 05:28 作者: Accrue 時(shí)間: 2025-3-27 13:28
Sequence-Defined Oligoaminoamides for the Delivery of siRNAs, displayed efficient siRNA-mediated gene silencing in vitro and in vivo. In this chapter, we provide a brief background on the special features of these polymers and detailed protocols to evaluate polyplex formation, gene silencing efficiency, and cytotoxicity of siRNA-containing polyplexes.作者: 不滿分子 時(shí)間: 2025-3-27 16:49 作者: forecast 時(shí)間: 2025-3-27 21:49
Identification and Validation of miRNA Target Sites Within Nontraditional miRNA Targets, predicting target sites within these RNAs. Here, the principles for developing computational algorithms for identifying putative miRNA target sites outside of mRNA are discussed. Laboratory techniques for validating computational miRNA target predictions are described.作者: Negligible 時(shí)間: 2025-3-28 00:33 作者: 一再遛 時(shí)間: 2025-3-28 04:27
nRIP-seq: A Technique to Identify RNA Targets of an RNA Binding Protein on a Genome-Wide Scale,nteraction between RNA and chromatin modifiers, Polycomb proteins, as an example to describe this method. Using nRIP-seq, we provide a snapshot of Ezh2, a Polycomb component, and RNA interaction in mouse embryonic stem cells.作者: Graduated 時(shí)間: 2025-3-28 06:30
Diverse Functions and Mechanisms of Mammalian Long Noncoding RNAs,iological processes. Here, we discuss their discovery, features, and known functions in cells. While not comprehensive, this chapter should serve to illustrate the power and promise of studying long noncoding RNAs.作者: heartburn 時(shí)間: 2025-3-28 11:28
Sequence-Defined Oligoaminoamides for the Delivery of siRNAs,s attention as a tool for gene silencing in basic science and as a novel therapeutic modality. To develop carriers for cytosolic and systemic siRNA delivery, our laboratory has recently developed a sequence-defined polymer platform compatible with solid-phase-supported synthesis. These polymers have作者: legislate 時(shí)間: 2025-3-28 15:39
A Biochemical Approach to Identify Direct MicroRNA Targets,y the tumor suppressor miRNA miR-34a. This method involves transfection of cells with biotinylated miRNA mimics, streptavidin pulldown, RNA isolation, and qRT-PCR. The protocol in this chapter describes these steps and the issues that should be considered while designing such pulldown experiments.作者: Foam-Cells 時(shí)間: 2025-3-28 19:17 作者: Factual 時(shí)間: 2025-3-29 01:52
Identification and Validation of miRNA Target Sites Within Nontraditional miRNA Targets,derstood to repress gene expression posttranscriptionally through recognition of 3′-untranslated regions (3′-UTRs) of mRNA transcripts, they have the potential to target additional classes of RNAs. Understanding the expanding pool of potential miRNA targets has been hindered by the lack of tools for作者: prostate-gland 時(shí)間: 2025-3-29 04:00 作者: Isometric 時(shí)間: 2025-3-29 10:03
,Affinity Purification of Long Noncoding RNA–Protein Complexes from Formaldehyde Cross-Linked Mammalsses. The purification and analysis of lncRNA–protein (lncRNP) complexes is critical toward understanding the normal physiological function of these molecules. Here, we describe the purification of lncRNP complexes from human cells using a FLAG-tagged MS2-phage coat protein (MS2 CP) that binds in se作者: BORE 時(shí)間: 2025-3-29 13:15 作者: epidermis 時(shí)間: 2025-3-29 16:36 作者: infarct 時(shí)間: 2025-3-29 21:34 作者: 贊成你 時(shí)間: 2025-3-30 00:02 作者: Delude 時(shí)間: 2025-3-30 06:58 作者: 松果 時(shí)間: 2025-3-30 10:09 作者: 彎腰 時(shí)間: 2025-3-30 13:00
On Using Magnesium and Potassium Ions in RNA Experiments,teract with potassium and magnesium ions. The role of magnesium ions has been extensively studied in RNA, as it is necessary for proper folding of RNA and acts as a nucleophile in many reactions catalyzed by RNA. A short overview of the role of magnesium and potassium in RNA structures is provided h作者: aggrieve 時(shí)間: 2025-3-30 18:49
Studying the Affinity, Kinetic Stability, and Specificity of RNA/Protein Interactions: SINE ncRNA/Pchallenge is to experimentally characterize these interactions to ultimately understand their biological roles at a mechanistic level, which will require a combination of multiple experimental techniques. One such category of techniques is biochemical assays that determine the affinity, kinetic stab作者: CHARM 時(shí)間: 2025-3-31 00:25
