標題: Titlebook: Recombineering; Methods and Protocol Christopher R. Reisch Book 2022 Springer Science+Business Media, LLC, part of Springer Nature 2022 CRI [打印本頁] 作者: 烹飪 時間: 2025-3-21 18:17
書目名稱Recombineering影響因子(影響力)
書目名稱Recombineering影響因子(影響力)學科排名
書目名稱Recombineering網(wǎng)絡公開度
書目名稱Recombineering網(wǎng)絡公開度學科排名
書目名稱Recombineering被引頻次
書目名稱Recombineering被引頻次學科排名
書目名稱Recombineering年度引用
書目名稱Recombineering年度引用學科排名
書目名稱Recombineering讀者反饋
書目名稱Recombineering讀者反饋學科排名
作者: Pillory 時間: 2025-3-21 21:31
Lambda Red Recombineering of Bacteriophage in the Lysogenic State,genome of a lysogenized host with a prophage compatible with bacteriophage lambda. Linear DNA is used as the recombination substrate and antibiotic resistance is used as the basis for selection of recombinants. The method enables the genetic manipulation of a prophage in 3–5?days.作者: 比賽用背帶 時間: 2025-3-22 03:09
Recombineering-Mediated Genome Editing in Burkholderiales Strains, also closely related Gram-negative bacteria for efficient genome editing. However, some distant bacterial species like Burkholderiales strains require host-specific Redα/Redβ recombinase pair for highly efficient genome editing. A pair of recombinases Redαβ7029 from the Burkholderiales strain DSM 7作者: 用不完 時間: 2025-3-22 06:47
High-Efficiency Multi-site Genomic Editing (HEMSE) Made Easy,chnology allows to introduce mutations within bacterial genomes in a very simple and straightforward way. This technology was initially developed for . but was later extended to other organisms of interest, including the environmentally and metabolically versatile .. The technology is based on three作者: epicondylitis 時間: 2025-3-22 09:16 作者: Hectic 時間: 2025-3-22 13:10
Plant Gene Modification by BAC Recombineering,rried by bacterial artificial chromosomes (BACs) in .. Whereas BAC transformation provides a simple means for integration of modified genes into the genomes of animal cells to generate knock-in and knockout lines, successful application of this strategy is hampered by low frequency of homologous rec作者: BOOST 時間: 2025-3-22 18:56
Dup-In and DIRex: Techniques for Single-Step, Scar-Free Mutagenesis with Marker Recycling, (DIRex). Dup-In is used for transferring existing mutations between strains, and DIRex for generating almost any type of mutation. Both techniques use intermediate insertions with counter-selectable cassettes, flanked by directly repeated sequences that enable exact and spontaneous excision of the 作者: Barrister 時間: 2025-3-23 01:13
Genome Editing in , Using CRISPR/Cas9 Technology,ave recently developed a CRISPR/Cas9-based genome editing system (pCasKP-pSGKP) by coupling a CRISPR/Cas9 system with the lambda Red recombination system as well as a cytidine deaminase-mediated base editing system (pBECKP) in ., enabling rapid, scarless, and efficient genetic manipulation in divers作者: 水獺 時間: 2025-3-23 04:56 作者: 歌曲 時間: 2025-3-23 06:13 作者: inclusive 時間: 2025-3-23 10:19 作者: locus-ceruleus 時間: 2025-3-23 15:56 作者: triptans 時間: 2025-3-23 18:46 作者: 異端邪說下 時間: 2025-3-24 02:01
CRISPR/Cas-Mediated Genome Editing of ,o proven to be functional chassis for the discovery and production of bioactive compounds and enzymes. However, genetic engineering of . is often laborious and time-consuming. Here we describe protocols for CRISPR/Cas-mediated genome editing of .. Starting from the design and assembly of all-in-one 作者: addition 時間: 2025-3-24 04:52
Cas9 Nickase-Based Genome Editing in , efficiency is quite low and, therefore, metabolically engineered strains with increased efficiency can decrease both the overall cost and time required for biofuel production. Traditional genetic tools are inefficient, expensive, and time-consuming, but recent developments in the use of CRISPR-Cas 作者: 溺愛 時間: 2025-3-24 08:54 作者: spinal-stenosis 時間: 2025-3-24 13:03
Scarless Recombineering of Phage in Lysogenic State,ls without a selection marker is feasible due to its high recombination frequency, estimated as more than 40% after six cycles. The method enables scarless editing of the genome of a bacteriophage in 4–5?days.作者: gnarled 時間: 2025-3-24 15:43
Recombineering-Mediated Genome Editing in Burkholderiales Strains,029, recently identified as ., were identified for efficient genetic manipulation in the native strain and several other Burkholderiales strains. In this chapter, we describe a detailed protocol for genome engineering in Burkholderiales strains via the Redγ-Redαβ7029 recombineering and Cre/loxP site-specific recombination.作者: vertebrate 時間: 2025-3-24 22:26 作者: 脆弱吧 時間: 2025-3-25 02:54
Book 2022 topics such as scarless DNA recombineering of phage in the lysogenic state; HEMSE; Dup-In and DIRex; recombineering in Staphylococcus aureus; and genome editing with Cas9 in lactobacilli. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions t作者: 使人煩燥 時間: 2025-3-25 04:10 作者: Proponent 時間: 2025-3-25 09:03
Genome Editing in , Using CRISPR/Cas9 Technology,tem as well as a cytidine deaminase-mediated base editing system (pBECKP) in ., enabling rapid, scarless, and efficient genetic manipulation in diverse . strains. In this chapter, we introduce the detailed procedures of using these two tools for genome editing in ..作者: COMA 時間: 2025-3-25 13:50
Genome Editing of , Using CRISPR-Cpf1 System,icroorganisms but cannot work well in .. We recently developed two . (.) CRISPR-Cpf1 assisted systems for genome editing via homologous recombination in .. Here, we describe the protocols and demonstrated that N iterative rounds of genome editing can be achieved in 3?.?+?4 or 3?.?+?2?days, respectively.作者: 災禍 時間: 2025-3-25 17:44
CRISPR/Cas-Mediated Genome Editing of ,rious and time-consuming. Here we describe protocols for CRISPR/Cas-mediated genome editing of .. Starting from the design and assembly of all-in-one CRISPR/Cas constructs for efficient double-strand break-mediated genome editing, we also present protocols for intergeneric conjugation, CRISPR/Cas plasmid curing, and validation of edited strains.作者: 廣大 時間: 2025-3-25 20:09 作者: outset 時間: 2025-3-26 00:36
Genome Editing Methods for , needed, strengths and limitations, time considerations, and troubleshooting notes to perform each method. Use of the methods outlined in this chapter will allow researchers to create gene insertions, deletions, substitutions, and RNA interference strains through a variety of methods custom to each application.作者: investigate 時間: 2025-3-26 07:52 作者: crucial 時間: 2025-3-26 10:12
Dup-In and DIRex: Techniques for Single-Step, Scar-Free Mutagenesis with Marker Recycling, selection for spontaneous loss of the counter-selectable cassette, which leaves only the intended mutation behind in the final strain. The techniques have been used in several strains of . and ., and should be readily adaptable to other organisms where λ Red recombineering or similar methods are available.作者: Celiac-Plexus 時間: 2025-3-26 16:24
Christopher R. ReischIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: milligram 時間: 2025-3-26 16:59 作者: audiologist 時間: 2025-3-27 00:50
https://doi.org/10.1007/978-1-0716-2233-9CRISPR; DNA nucleases; genotyping; Escherichia coli; Gram-Negative Bacteria; Gram-Positive Bacteria; recom作者: 博識 時間: 2025-3-27 02:00
978-1-0716-2235-3Springer Science+Business Media, LLC, part of Springer Nature 2022作者: 易于出錯 時間: 2025-3-27 07:36
Recombineering978-1-0716-2233-9Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Simulate 時間: 2025-3-27 12:53
Lambda Red Recombineering of Bacteriophage in the Lysogenic State,genome of a lysogenized host with a prophage compatible with bacteriophage lambda. Linear DNA is used as the recombination substrate and antibiotic resistance is used as the basis for selection of recombinants. The method enables the genetic manipulation of a prophage in 3–5?days.作者: 抱負 時間: 2025-3-27 13:43 作者: 苦惱 時間: 2025-3-27 18:46
Piyush Behari Lal,Fritz Wells,Patricia J. Kiley-box nature of neural models. We show that nilpotent logical systems studied in the previous chapters offer an appropriate mathematical framework for the hybridization of continuous nilpotent logic and neural models, and help improve the interpretability and safety of machine learning. In this appro作者: 難聽的聲音 時間: 2025-3-28 00:01
Zhoubo Hu,Ajit Ghosh,Csaba Konczcus on nilpotent logical systems and describe aggregative operators in such systems ranging from symmetric ones (that treat all the inputs equally) to weighted ones, where some inputs are given more weight than others. As a starting point, instead of associativity, we focus on the necessary and suff作者: Alpha-Cells 時間: 2025-3-28 03:40
Joakim N?svallcus on nilpotent logical systems and describe aggregative operators in such systems ranging from symmetric ones (that treat all the inputs equally) to weighted ones, where some inputs are given more weight than others. As a starting point, instead of associativity, we focus on the necessary and suff作者: Conflict 時間: 2025-3-28 08:56
Zhipeng Wang,Yu Wang,Quanjiang Jicus on nilpotent logical systems and describe aggregative operators in such systems ranging from symmetric ones (that treat all the inputs equally) to weighted ones, where some inputs are given more weight than others. As a starting point, instead of associativity, we focus on the necessary and suff作者: Vertebra 時間: 2025-3-28 10:49
Adam J. Ellington,Christopher R. Reischking the choices that are needed in order to fully specify an IT2 fuzzy system, introduces two approaches to design—the partially dependent approach and the totally independent approach (but this time for singleton, T1 non-singleton, and IT2 non-singleton IT2 fuzzy systems), includes some important 作者: 輕推 時間: 2025-3-28 17:13 作者: 補角 時間: 2025-3-28 22:35
Katherine J. Wozniak,Lyle A. Simmons context in which explanation methods can be evaluated regarding their adequacy. The goal of this chapter is to bridge the gap between expert users and lay users. Different kinds of users are identified and their concerns revealed, relevant statements from the General Data Protection Regulation are 作者: hypnogram 時間: 2025-3-29 00:20
Wei Hong,Jie Zhang,Guzhen Cui,Qian Zhou,Pixiang Wang,Yi Wang provide easy-to-interpret rationales for their behavior—so that passengers, insurance companies, law enforcement, developers etc., can understand what triggered a particular behavior. Here, we explore the use of visual explanations. These explanations take the form of real-time highlighted regions 作者: Disk199 時間: 2025-3-29 06:39
Zhiqiang Wen,Fenghui Qian,Jiao Zhang,Yu Jiang,Sheng Yangman-machine interactions. Expressing the rationale behind such a system’s output is an important aspect of human-machine interaction as AI continues to be prominent in general, everyday use-cases. In this paper, we introduce a novel framework integrating Grenander’s pattern theory structures to prod作者: Instinctive 時間: 2025-3-29 08:10 作者: 過份好問 時間: 2025-3-29 13:43 作者: 向下 時間: 2025-3-29 19:04
Justin M. Vento,Chase L. Beiselg to some of the attributes in our classification system, summarized in Tables?., ., . and .. The methods in each category are described and discussed. The categories are Decision Tree, Single Decision Tree, Single Altered Decision Tree, Multiple or Combined Decision Trees, Saliency Maps, Post-Hoc S作者: 小丑 時間: 2025-3-29 23:01
help facilitate discussion of and research into this interdisciplinary field. We consider classification techniques used in past surveys and papers and attempt to unify terminology, describing 12 attributes that can be used to classify explainable/interpretable techniques: (i) Model-Agnostic or Mod作者: 正面 時間: 2025-3-30 00:59 作者: Exhilarate 時間: 2025-3-30 05:16 作者: 分開如此和諧 時間: 2025-3-30 10:12 作者: Stress-Fracture 時間: 2025-3-30 13:40
Joakim N?svallnt types of operators using only one generator function. The formula also contains a parameter with the semantical meaning of the threshold of expectancy. Interestingly, the resulting formula turns out to be equivalent to that used in current deep learning techniques.作者: 首創(chuàng)精神 時間: 2025-3-30 17:12
Zhipeng Wang,Yu Wang,Quanjiang Jint types of operators using only one generator function. The formula also contains a parameter with the semantical meaning of the threshold of expectancy. Interestingly, the resulting formula turns out to be equivalent to that used in current deep learning techniques.作者: Homocystinuria 時間: 2025-3-30 21:54
Adam J. Ellington,Christopher R. Reischence system—ANFIS, and structure identification and feature extraction—SIFE—for TSK systems); and the continuation of the Chap. . five case studies from T1 to IT2 fuzzy systems (forecasting of time series, knowledge mining using surveys, rule-based classification of video traffic, fuzzy logic contro作者: Friction 時間: 2025-3-31 01:00 作者: 圓錐 時間: 2025-3-31 08:02
Katherine J. Wozniak,Lyle A. Simmonsare missing and we speculate which criteria these methods/interfaces should satisfy. Finally it is noted that two important concerns are difficult to address with explanation methods: the concern about bias in datasets that leads to biased DNNs, as well as the suspicion about unfair outcomes.作者: ANTI 時間: 2025-3-31 12:31
