標(biāo)題: Titlebook: Recombinant and In Vitro RNA Synthesis; Methods and Protocol Graeme L. Conn Book 2012 Springer Science+Business Media, LLC 2012 RNA purific [打印本頁(yè)] 作者: Concave 時(shí)間: 2025-3-21 16:40
書(shū)目名稱(chēng)Recombinant and In Vitro RNA Synthesis影響因子(影響力)
書(shū)目名稱(chēng)Recombinant and In Vitro RNA Synthesis影響因子(影響力)學(xué)科排名
書(shū)目名稱(chēng)Recombinant and In Vitro RNA Synthesis網(wǎng)絡(luò)公開(kāi)度
書(shū)目名稱(chēng)Recombinant and In Vitro RNA Synthesis網(wǎng)絡(luò)公開(kāi)度學(xué)科排名
書(shū)目名稱(chēng)Recombinant and In Vitro RNA Synthesis被引頻次
書(shū)目名稱(chēng)Recombinant and In Vitro RNA Synthesis被引頻次學(xué)科排名
書(shū)目名稱(chēng)Recombinant and In Vitro RNA Synthesis年度引用
書(shū)目名稱(chēng)Recombinant and In Vitro RNA Synthesis年度引用學(xué)科排名
書(shū)目名稱(chēng)Recombinant and In Vitro RNA Synthesis讀者反饋
書(shū)目名稱(chēng)Recombinant and In Vitro RNA Synthesis讀者反饋學(xué)科排名
作者: 細(xì)微差別 時(shí)間: 2025-3-22 00:08
Christopher J. Kershaw,Raymond T. O’Keefe erprobten innovativen Management-Ansatz.Ein Buch von PraktiDieses Buch zeigt, dass wirksames Kulturmanagement vor allem darauf abzielt, bereits vorhandene positive Muster der Unternehmenskultur zu identifizieren und diese im Sinne des heutigen und zukünftigen Erfolgs gezielt zu st?rken. Es geht fol作者: ALTER 時(shí)間: 2025-3-22 01:57 作者: 木訥 時(shí)間: 2025-3-22 07:10 作者: glacial 時(shí)間: 2025-3-22 10:47 作者: 種族被根除 時(shí)間: 2025-3-22 13:57 作者: gimmick 時(shí)間: 2025-3-22 18:29
Recombinant and In Vitro RNA Synthesis978-1-62703-113-4Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: FECK 時(shí)間: 2025-3-22 22:51 作者: antenna 時(shí)間: 2025-3-23 04:47 作者: Substitution 時(shí)間: 2025-3-23 07:33
Graeme L. ConnDetails a collection of protocols that guide the reader from the start of a new RNA project to the final sample.Provides step-by-step detail essential for reproducible results.Contains key notes and i作者: labile 時(shí)間: 2025-3-23 12:08 作者: pellagra 時(shí)間: 2025-3-23 17:24
Affinity Purification of RNA Using an ARiBo Tag,. ribozyme with glucosamine-6-phosphate. This Chapter outlines the experimental details for affinity batch purification of RNAs using ARiBo tags. Although the procedure was originally developed for purification of a stable purine riboswitch mutant, it is demonstrated here for purification of the ter作者: 阻塞 時(shí)間: 2025-3-23 20:19
,Plasmid Template Design and In Vitro Transcription of Short RNAs Within a “Structure Cassette” for ethods by which any RNA of interest may be cloned into a new plasmid preloaded with sequences that encode a structure cassette surrounding the short internal target RNA. Next, we outline key considerations and analyses of the RNAs produced that should be performed prior to SHAPE or other structure p作者: 投票 時(shí)間: 2025-3-24 00:50 作者: 猛擊 時(shí)間: 2025-3-24 03:58
Lina Lin,Zhen Huangs umfassende Werk zur Histotechnik deckt die Anforderungen der Fachhochschule bzw. Akademie für Biomedizinische Analytik ab, ist für jeden Studenten der Medizin als Einblick in die Histotechnik geeignet und empfiehlt sich als Standardnachschlagewerk für alle, die mit Gewebe arbeiten..作者: 侵蝕 時(shí)間: 2025-3-24 08:45
Book 2012tep, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls..?.Authoritative and practical, .Recombinant and In Vitro RNA Synthesis: Methods and Protocols. aids scientists in continuing to study the fascinating world of RNA..作者: dialect 時(shí)間: 2025-3-24 13:12
Luc Ponchon,Geneviève Beauvais,Sylvie Nonin-Lecomte,Frédéric Dardel作者: 受傷 時(shí)間: 2025-3-24 15:58
Jessica C. Bowman,Bahareh Azizi,Timothy K. Lenz,Poorna Roy,Loren Dean Williams作者: considerable 時(shí)間: 2025-3-24 19:36 作者: 名字的誤用 時(shí)間: 2025-3-25 01:26
Geneviève Di Tomasso,Philipe Lampron,James G. Omichinski,Pascale Legault作者: 征兵 時(shí)間: 2025-3-25 05:16 作者: 使殘廢 時(shí)間: 2025-3-25 11:17 作者: hyperuricemia 時(shí)間: 2025-3-25 14:57
Christopher J. Kershaw,Raymond T. O’Keefer und beschreiben aus welchen fünf zentralen Fragmenten sich diese zusammensetzt. Anschlie?end stellen sie ihren vielfach in der Praxis erprobten Prozess vor, der Kultur sichtbar, verst?ndlich und damit für all978-3-658-42768-9978-3-658-42769-6作者: 破譯 時(shí)間: 2025-3-25 17:40 作者: Yourself 時(shí)間: 2025-3-25 23:21 作者: 浸軟 時(shí)間: 2025-3-26 03:59 作者: HEAVY 時(shí)間: 2025-3-26 05:38 作者: 流出 時(shí)間: 2025-3-26 09:42
General Protocols for Preparation of Plasmid DNA Template, RNA In Vitro Transcription, and RNA Purier the last three decades. The numerous approaches available today to study RNA structure and function vary widely in their demands on the quality and quantity of material needed. These range for example from a few micrograms in biochemical assays, RNA structure probing or RNA folding studies using 作者: Mangle 時(shí)間: 2025-3-26 13:15
Preparation of Short RNA by In Vitro Transcription,t of specific RNA sufficient for structural studies in a more economical way than chemical synthesis. Here, we provided protocols optimized for the synthesis of very short RNA of 10–20 nucleotides in length by in vitro transcription. These RNA oligomers can be purified conveniently by gel filtration作者: 氣候 時(shí)間: 2025-3-26 17:09 作者: bronchodilator 時(shí)間: 2025-3-26 21:04
,,-Acting Ribozymes for the Production of RNA In Vitro Transcripts with Defined 5′ and 3′ Ends, at both the 5′- and 3′-ends. This chapter describes the use of .-acting ribozymes, 5′-end hammerhead (HH) and 3′-end hepatitis delta virus (HDV), for direct transcriptional processing to yield target RNAs with precisely defined ends. The method is focused on the use of the pRZ and p2RZ plasmids tha作者: phase-2-enzyme 時(shí)間: 2025-3-27 03:41 作者: 使尷尬 時(shí)間: 2025-3-27 08:22
Rapid Preparation of RNA Samples Using DNA-Affinity Chromatography and DNAzyme Methods,ription method results in heterogeneity at the RNA 3′-terminus. RNA purification requires single-nucleotide resolution to separate the transcript of the correct length from the aborted or add-on transcripts that are usually present in comparable amounts. Here, we describe an RNA preparation method t作者: Predigest 時(shí)間: 2025-3-27 13:22
,Preparation of λN-GST Fusion Protein for Affinity Immobilization of RNA, Affinity purification is particularly advantageous because it can be performed in a few hours under non-denaturing conditions. However, the performance of affinity purification methods can vary tremendously depending on the RNA immobilization matrix. It was previously shown that RNA immobilization 作者: 受人支配 時(shí)間: 2025-3-27 17:20 作者: dissent 時(shí)間: 2025-3-27 20:25 作者: Engulf 時(shí)間: 2025-3-28 00:12
In Vitro Transcription of Modified RNAs,o standard transcription protocols. RNAs containing a single phosphate or a cap structure at their 5′ ends can readily be generated either co-transcriptionally or through enzymatic treatments of transcription products. Likewise, a variety of modified bases, including fluorescent or biotinylated spec作者: medium 時(shí)間: 2025-3-28 05:21
End-Labeling Oligonucleotides with Chemical Tags After Synthesis,le nucleic acid detection, yield information about its state, and can serve as a handle by which the nucleic acid and associated factors can be purified from a mixture. Radioactive phosphate is commonly added to the 5′ or 3′ end of an oligonucleotide post synthesis using enzyme-catalyzed reactions. 作者: abduction 時(shí)間: 2025-3-28 08:11
High-Purity Enzymatic Synthesis of Site-Specifically Modified tRNA,ndent on the presence of modified nucleotides in tRNA, each of which performs a distinct function. To better understand how individual modifications modulate tRNA function, a method to isolate and purify a site-specifically modified tRNA is essential. This chapter describes an enzymatic method to sy作者: 懶鬼才會(huì)衰弱 時(shí)間: 2025-3-28 10:28 作者: 充氣女 時(shí)間: 2025-3-28 17:28 作者: 窒息 時(shí)間: 2025-3-28 19:17
Preparative Separation of Ribonucleoside Monophosphates by Ion-Pair Reverse-Phase HPLC,g these, nucleotide-specific and site-specific labeling methods can help tremendously in simplifying complex NMR data, while providing unique opportunities for structural investigation of larger RNAs. Such methods generally require separation of individual isotopically labeled ribonucleoside monopho作者: 障礙物 時(shí)間: 2025-3-29 02:11 作者: 致敬 時(shí)間: 2025-3-29 05:38
Preparation of Short RNA by In Vitro Transcription,nthesis of very short RNA of 10–20 nucleotides in length by in vitro transcription. These RNA oligomers can be purified conveniently by gel filtration chromatography. We also described how to study RNA and protein binding by gel filtration chromatography.作者: Acumen 時(shí)間: 2025-3-29 10:43
Native RNA Purification by Gel Filtration Chromatography,fication using Superdex 75 or 200 gel filtration columns. This approach can be extended to purify biologically interesting RNA complexes such as RNA–protein complexes that have been generated from either synthetic or in vitro transcribed RNAs and recombinant proteins.作者: 確定無(wú)疑 時(shí)間: 2025-3-29 15:02 作者: 沉著 時(shí)間: 2025-3-29 18:13 作者: 表臉 時(shí)間: 2025-3-29 20:31
Biosynthetic Preparation of 13C/15N-Labeled rNTPs for High-Resolution NMR Studies of RNAs,hion from bacterial cultures, using common and versatile . strains. This chapter also covers procedures for extraction and digestion of the total RNA from bacterial cells, purification of the ribonucleoside 5′-monophosphates and their enzymatic phosphorylation to rNTPs.作者: 男生如果明白 時(shí)間: 2025-3-30 01:57
Splint Ligation of RNA with T4 DNA Ligase,in an RNA and for the assembly of smaller synthetic RNAs into longer RNA molecules. Such modifications enable a wide range of experiments to be carried out with the modified RNA including structural studies, co-immunoprecipitations, and the ability to map sites of RNA:RNA and RNA:protein interactions.作者: Watemelon 時(shí)間: 2025-3-30 06:41 作者: Arthropathy 時(shí)間: 2025-3-30 11:18
General Protocols for Preparation of Plasmid DNA Template, RNA In Vitro Transcription, and RNA Puriaphy. Therefore, robust and scalable protocols, such as those described in this chapter, for production of plasmid DNA template, RNA in vitro transcription, and RNA purification, are an essential component of any RNA laboratory’s experimental repertoire.作者: 小卷發(fā) 時(shí)間: 2025-3-30 14:39 作者: 使絕緣 時(shí)間: 2025-3-30 18:16
,Preparation of λN-GST Fusion Protein for Affinity Immobilization of RNA,Chapter outlines the experimental procedure employed to prepare the λN-GST fusion protein used for RNA immobilization in successful affinity purifications of RNA. It describes the details of protein expression and purification as well as routine quality control analyses.作者: expdient 時(shí)間: 2025-3-30 23:19
In Vitro Transcription of Modified RNAs, incorporation of modified base relative to its standard counterpart. Finally, an approach to place a poly(A) tail at the exact 3′ end of a desired transcription product is presented. Collectively, these protocols allow one to synthesize RNAs with a variety of modifications to serve as versatile molecules to analyze biological questions.作者: 正論 時(shí)間: 2025-3-31 04:30 作者: harbinger 時(shí)間: 2025-3-31 06:33
1064-3745 essential for reproducible results.Contains key notes and i.The discovery of catalytic RNAs in the mid-1980s marked the beginning of a new era in RNA biology and an ever increasing appreciation of the diverse and critical roles played by this fascinating molecule. In. .Recombinant and In Vitro RNA 作者: Prosaic 時(shí)間: 2025-3-31 10:29
Microbiologye for cultures and came to the conclusion that it was infinite. We were faced with designing a system which incorporated the advantages of nursing station ordering along with the flexibility of free-format English.作者: Crohns-disease 時(shí)間: 2025-3-31 16:13
Estimation Based Adaptive ACB Scheme for M2M Communications,to the network load conditions. At the same time, we summarize the boundedness of the adaptive scheme. Finally, simulation results illustrate that our scheme can increase the system throughput and reduce access delay obviously.作者: 絆住 時(shí)間: 2025-3-31 17:33
Index der Krystallformen der Mineralien obvious, acute effects. Furthermore, nutrient enrichment interacts with other major drivers, such as hydrology, food web interactions, and climate change, in both direct and indirect ways. Many questions remain, however. Much needs to be done in parameterizing rates, characterizing traits, and how 作者: lipids 時(shí)間: 2025-4-1 01:15
Grace Davieerrt sich ja der Histomat auf weiteste Strecken jeder originelleren Ausarbeitung; daran ?ndert auch die Tatsache nichts, da? eine kritische Gesamtdarstellung des sowjetischen Histomat (etwa in der Art der bekannten Arbeiten von Wetter, Bocheński u.a. über den Diamat) noch immer ein Desiderat der wes作者: Exploit 時(shí)間: 2025-4-1 02:16
IDENTIFICATION OF COMPLEX PROCESSES BASED ON ANALYSIS OF PHASE SPACE STRUCTURES,tc. The past two decades of research on qualitative and especially quantitative investigations of dynamics of real processes of different origin brought significant progress in the understanding of behavior of natural processes. At the same time serious drawbacks have also been revealed.作者: 描述 時(shí)間: 2025-4-1 08:45
1876-1100 udents, researchers and professionals interested in the digital transformation of manufacturing domains and work environments.?.978-3-030-48604-4978-3-030-48602-0Series ISSN 1876-1100 Series E-ISSN 1876-1119 作者: Assignment 時(shí)間: 2025-4-1 12:17 作者: 充滿人 時(shí)間: 2025-4-1 15:56
Christopher Adair-Toteffents..What You‘ll Learn.Work with the core fundamentals of Go.Persist data into NoSQL databases.Build scalable backend APIs.Test your Go applications.Create HTTP web servers in Go.Who This Book Is For.Experienced programmers who have some or no prior experience with Go..978-1-4842-1189-2978-1-4842-1188-5作者: obscurity 時(shí)間: 2025-4-1 19:00
0947-2657 he volumes focus on topics that have developed a solid data and conceptual foundation rather than on those for which a literature is only beg- ning to develop. New research areas will be covered on a timely basis in the series as they begin to mature.978-1-4614-2649-3978-1-4419-6114-3Series ISSN 0947-2657 Series E-ISSN 2197-1897
