標(biāo)題: Titlebook: Recombinant Protein Protocols; Detection and Isolat Rocky S. Tuan Book 1997 Springer Science+Business Media New York 1997 [打印本頁] 作者: GLOAT 時間: 2025-3-21 18:34
書目名稱Recombinant Protein Protocols影響因子(影響力)
書目名稱Recombinant Protein Protocols影響因子(影響力)學(xué)科排名
書目名稱Recombinant Protein Protocols網(wǎng)絡(luò)公開度
書目名稱Recombinant Protein Protocols網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Recombinant Protein Protocols被引頻次
書目名稱Recombinant Protein Protocols被引頻次學(xué)科排名
書目名稱Recombinant Protein Protocols年度引用
書目名稱Recombinant Protein Protocols年度引用學(xué)科排名
書目名稱Recombinant Protein Protocols讀者反饋
書目名稱Recombinant Protein Protocols讀者反饋學(xué)科排名
作者: Living-Will 時間: 2025-3-21 22:36
Rocky S. Tuanhang gebracht werden. über spezielle Funktionen in den CAD/CAM-Systemen (zB. Reporter-Funktion bei CATIA) k?nnen nicht grafische Informationen, die über Attribute erfa?t wurden, ausgewertet und damit umfassende Fertigungs- und Steuerungsunterlagen (Stücklistenerstellung, NC-Programmierung usw.) erst作者: 使成核 時間: 2025-3-22 03:41 作者: Brain-Imaging 時間: 2025-3-22 07:36
Peiyu Lee,Dennis E. Hrubyhang gebracht werden. über spezielle Funktionen in den CAD/CAM-Systemen (zB. Reporter-Funktion bei CATIA) k?nnen nicht grafische Informationen, die über Attribute erfa?t wurden, ausgewertet und damit umfassende Fertigungs- und Steuerungsunterlagen (Stücklistenerstellung, NC-Programmierung usw.) erst作者: 朝圣者 時間: 2025-3-22 11:53 作者: Ebct207 時間: 2025-3-22 16:05
Steven R. Kainf Seite 9). Dabei wurden Verfahren entwickelt, die für Bilder aus den verschiedensten Anwendungsbereichen einsetzbar sind. Für solche Verfahren wurden diverse Programmpakete erstellt (z.B. SPIDER, vgl. ?SPIDER — ein Software-Paket für Bildverarbeitung und Bildanalyse? auf Seite 13). Diese k?nnen an 作者: Decimate 時間: 2025-3-22 17:24 作者: 現(xiàn)存 時間: 2025-3-22 22:42
Corinne E. M. Olesen,John C. Voyta,Irena Bronstein作者: AV-node 時間: 2025-3-23 02:43 作者: synovial-joint 時間: 2025-3-23 08:04 作者: 簡略 時間: 2025-3-23 11:30 作者: 小平面 時間: 2025-3-23 13:54 作者: 弄污 時間: 2025-3-23 20:26 作者: 漂泊 時間: 2025-3-24 00:40 作者: 丑惡 時間: 2025-3-24 02:50
Book 1997of methodologies to enhance the detection and isolation of their gene product(s) of interest. A large number of molecular tags and labels and their usage are described, including enzymes, ligand-binding moieties, immunodetectable molecules, as well as methods to detect interactive proteins, and gene作者: Brain-Waves 時間: 2025-3-24 07:02 作者: hermetic 時間: 2025-3-24 12:52
Methods in Molecular Biologyhttp://image.papertrans.cn/r/image/824103.jpg作者: THROB 時間: 2025-3-24 16:22 作者: chemoprevention 時間: 2025-3-24 22:13
Detection of β-Galactosidase and β-Glucuronidase Using Chemiluminescent Reporter Gene Assaystivity with a variety of detection techniques including radioisotopic, colorimetric, luminescent, fluorescent, and immunoassay methods (.–.). Chemiluminescent assays have been very effective in detecting enzyme labels in direct enzyme activity assays, immunoassays, and nucleic acid hybridization assays.作者: 法律 時間: 2025-3-25 00:32
Detection and Selection of Cultured Cells Secreting Recombinant Product by Soft Agar Cloning and Antvels of heterologous protein. The combination of cloning and selection speeds up the generation of permanent cell lines, increases the number of potential insertion events that can be screened, and reduces the tedium of sequentially cloning then analyzing the production levels of each clone.作者: 能得到 時間: 2025-3-25 06:23
Detection and Isolation of Recombinant Proteins Based on Binding Affinity of Reporter: Protein Ae first described system allowing affinity purification of expressed gene products. To date, a multitude of proteins have been produced as fusions to the IgG-binding domains of staphylococcal protein A, in several different hosts such as gram-positive and gram-negative bacteria (.,.), yeast (.), CHO cells (.), insect cells (.) and plants (.).作者: olfction 時間: 2025-3-25 11:02
978-1-4899-4167-1Springer Science+Business Media New York 1997作者: FELON 時間: 2025-3-25 14:16
Recombinant Protein Protocols978-1-59259-549-5Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 寬容 時間: 2025-3-25 15:51
1064-3745 s of recombinant gene products cover a wide spectrum, including gene therapy, production of bioactive pharmaceuticals, synthesis of novel biopolymers, agriculture and animal husbandry, and so on. Inherent in bringing these appli- cations to fruition is the need to design "expression constructs" that作者: 白楊魚 時間: 2025-3-25 20:01 作者: TRAWL 時間: 2025-3-26 02:53
Expression and Purification of Recombinant Streptavidin-Containing Chimeric Proteinsr a variety of biological and biomedical analyses (.,.). The ability of biotin to be incorporated easily into various biological materials has also expanded the application of the streptavidin-biotin technology to a wider range of biological systems.作者: 感染 時間: 2025-3-26 04:59
Detection and Isolation of Recombinant Proteins from Mammalian Cells by Immunoaffinity Chromatographpitope on the recombinant protein is mapped to a short primary sequence a synthetic epitope peptide can be used to elute the protein from the antibody under mild conditions. This technique has been employed to elute proteins from immunoaffinity columns with the retention of biological function.作者: 惹人反感 時間: 2025-3-26 09:44
Alternative Yeast Two-Hybrid Systemsng cDNA libraries for novel interacting proteins, and screening novel proteins against predetermined sets of known proteins to identify which pairs interact. We here describe strategies for these purposes utilizing the interaction trap (.), a two-hybrid system variant developed in the laboratory of Roger Brent (.).作者: 輕信 時間: 2025-3-26 14:49 作者: 加入 時間: 2025-3-26 17:39
Detection of Heterologous Gs-Coupled Receptor Activity in LLC-PK1 Cells Based on Expression of Urokie visualized . by overlaying colonies of stimulated cells with agar containing both plasminogen and casein (.). The degradation of the milk-protein casein by plasmin leads to the formation of lytic zones that allow the identification of uPA secreting colonies.作者: 全部逛商店 時間: 2025-3-27 00:41
Histochemical and Fluorochrome-Based Detection of β-Galactosidasestaining fixed specimens, and the other for staining having cells. Each of these techniques has unique advantages and limitations, but together they offer a wide range of possibilities for taking advantage of β-gal as a reporter enzyme in a variety of applications.作者: 優(yōu)雅 時間: 2025-3-27 02:49 作者: 平項山 時間: 2025-3-27 05:42 作者: 富饒 時間: 2025-3-27 11:43
Chemiluminescent Immunoassay for the Detection of Chloramphenicol Acetyltransferase and Human Growthn used with Sapphire-II enhancing reagent for enzyme-linked immunoassay detection of chloramphenicol acetyltransferase and human growth hormone reporter gene products. These chemiluminescent reporter gene assay systems provide nonradioactive, simple, sensitive detection methods, performed in microplate or tube luminometers.作者: 彎曲的人 時間: 2025-3-27 15:41 作者: Exposure 時間: 2025-3-27 18:15
Reporter Systemsreporter system is and outline the many uses of genetic reporters. Furthermore, the currently available reporter genes and assays will be described in terms of their specific applications and limitations.作者: 受傷 時間: 2025-3-28 00:55 作者: FAWN 時間: 2025-3-28 05:02
Human Placental Alkaline Phosphatase as a Marker for Gene Expressionns. To achieve this, it is frequently convenient to place a marker (or surrogate) gene under the genetic control of the regulatory sequences of interest. Expression of the marker gene is then monitored by analyzing accumulation of the encoded protein and this, in turn, serves as a measure of gene ex作者: Heart-Rate 時間: 2025-3-28 06:24
Use of Secreted Alkaline Phosphatase as a Reporter of Gene Expression in Mammalian Cellsments in transfected cells. As a reporter of gene expression, SEAP differs from intracellular reporters such as chloramphenicol acetyltransferase (CAT) and firefly luciferase in that SEAP is secreted from transfected cells. SEAP can thus be assayed using a small portion of the culture medium. The SE作者: epinephrine 時間: 2025-3-28 14:09 作者: BRAVE 時間: 2025-3-28 17:02
Chemiluminescent Immunoassay for the Detection of Chloramphenicol Acetyltransferase and Human Growthimmunoassays for highly sensitive detection of many analytes (.–.). CSPD chemiluminescent 1,2-dioxetane substrate (.) for alkaline phosphatase has been used with Sapphire-II enhancing reagent for enzyme-linked immunoassay detection of chloramphenicol acetyltransferase and human growth hormone report作者: bronchiole 時間: 2025-3-28 22:42 作者: 溫和女孩 時間: 2025-3-29 00:12
Detection and Isolation of Recombinant Protein Based on Binding Affinity Reporternserted downstream from the . gene of ., which encodes maltose-binding protein (MBP), resulting in the expression of an MBP fusion protein (.,.). The method uses the strong “tat” promoter and the . translation initiation signals to give high-level expression of the cloned sequences (.,.), and a one-作者: fluoroscopy 時間: 2025-3-29 04:03
Detection and Isolation of Recombinant Proteins Based on Binding Affinity of Reporter: Protein Ae first described system allowing affinity purification of expressed gene products. To date, a multitude of proteins have been produced as fusions to the IgG-binding domains of staphylococcal protein A, in several different hosts such as gram-positive and gram-negative bacteria (.,.), yeast (.), CHO作者: Overdose 時間: 2025-3-29 09:33
Expression and Purification of Recombinant Streptavidin-Containing Chimeric Proteinstant of the streptavidin-biotin complex is approx 10..; the binding of streptavidin to biotin is one of the strongest noncovalent interactions found in biological systems. The extremely tight and specific biotin-binding ability of streptavidin has made this protein a very powerful biological tool fo作者: 五行打油詩 時間: 2025-3-29 11:26
Bacterial Expression, Purification, and Potential Use of His-Tagged GAL4 Fusion Proteins of proteins and to characterize and clone interacting proteins (.,.). The yeast transcriptional activator protein GAL4 contains the sequences necessary for DNA-binding, dimerization and nuclear localization within its N-terminal 147 amino acids (.). A dimer of this fragment can bind specifically to作者: 珍奇 時間: 2025-3-29 16:13
Detection of Expressed Recombinant Protein Based on Multidrug Resistance: P-Glycoproteinphenomenon of MDR was first observed in the clinic in tumors of patients undergoing chemotherapy. Multidrug resistance was recognized as a major factor contributing to the failure of chemotherapeutic treatment of cancer (.). Multidrug resistance also occurs in cultured cells selected for resistance 作者: Delectable 時間: 2025-3-29 23:29
Detection and Isolation of Recombinant Proteins from Mammalian Cells by Immunoaffinity Chromatographs the reduced number of steps required for purification. However, owing to the high avidity of antibodies for their respective antigens, it is often difficult to elute the recombinant protein from the immunoaffinity column without denaturing the antibody or protein antigen. If the antibody binding e作者: 追逐 時間: 2025-3-30 00:23
Yeast GAL4 Two-Hybrid System The system offers several innovations for identifying interacting proteins over conventional biochemical methods such as coimmunoprecipitation and affinity copurification. The two-hybrid system is the first genetic and molecular approach to detect interacting proteins, and the assay is performed in作者: Foam-Cells 時間: 2025-3-30 06:18
Alternative Yeast Two-Hybrid SystemsBD) fused P1 and activation-domain (AD) fused P2 by assaying transcriptional activation of one or more reporter genes containing cognate DNA binding site for the DBD (.), a number of groups have developed variants of this approach for purposes such as mapping interaction domains on proteins, screeni作者: assail 時間: 2025-3-30 10:21
Detection of Heterologous Gs-Coupled Receptor Activity in LLC-PK1 Cells Based on Expression of Urokitimulate intracellular CAMP synthesis (.). The increase of CAMP leads to a 200-fold activation of uPA gene transcription (.). In response to optimal concentrations of the peptide hormone calcitonin, i.e., for which the cells express a G.-coupled receptor (.,.), high levels of uPA protein are synthes作者: Formidable 時間: 2025-3-30 15:57
Histochemical and Fluorochrome-Based Detection of β-Galactosidaselly useful in transgenic and chimeric mouse studies. In such whole-animal applications, the experimental goals often include the evaluation of the spatial and tissue specific patterns of reporter gene expression .. In such instances, the LacZ reporter gene has the advantage over other reporters in t作者: Incorporate 時間: 2025-3-30 18:30
Combined, Sequential , Hybridization and Immunohistochemistry on the Same Tissue Sectiont localizes mRNA transcripts in particular cell populations using a labeled nucleic acid probe; and immunohistochemistry (IMH), that detects protein gene products via labeled primary or secondary antibodies. Often, ISH and IMH are performed separately on adjacent serial sections, allowing comparison作者: 避開 時間: 2025-3-30 21:00 作者: CAND 時間: 2025-3-31 03:08 作者: municipality 時間: 2025-3-31 07:06 作者: 走路左晃右晃 時間: 2025-3-31 11:05
Peiyu Lee,Dennis E. Hrubye ist die gemeinsame Benutzeroberfl?che der beiden Systeme KI und CAD/CAM, bei der ein Dialog zwischen dem Anwender und dem Kl-Werkzeug umfassend aus dem CAD/CAM-Umfeld unterstützt wird. Informationen an das Kl-System k?nnen durch Geometrieselektion in dem CAD/CAM-System erfa?t und an das Kl-System 作者: 礦石 時間: 2025-3-31 16:08 作者: Override 時間: 2025-3-31 21:00 作者: LINES 時間: 2025-3-31 22:37
Corinne E. M. Olesen,John J. Fortin,John C. Voyta,Irena Bronsteinentwickelt wurde. Der Gebrauch dieses Expertensystems wurde anhand einiger Beispiele erl?utert..Ausgehend von der Erkenntnis, dass das in Expertensystemen für den Einsatz von Subroutinenpaketen ben?tigte Wissen bzgl. seines Gültigkeitsbereichs in 3 Ebenen aufgeteilt werden kann, wurde zuerst eine Ex作者: 出處 時間: 2025-4-1 02:39
Textbook 2006in rule, which gives the derivative of a composite of two di?erentiable functions. The chain rule, when written in an inde?nite integral form, yields the method of substitution. In advanced calculus, the Riemann–Stieltjes integral is de?ned through the same procedure of “partition-evaluation-summati作者: 元音 時間: 2025-4-1 07:55 作者: 極大痛苦 時間: 2025-4-1 12:30
