標題: Titlebook: Recombinant Protein Production in Yeast; Brigitte Gasser,Diethard Mattanovich Book 2019 Springer Science+Business Media, LLC, part of Spri [打印本頁] 作者: Intimidate 時間: 2025-3-21 19:27
書目名稱Recombinant Protein Production in Yeast影響因子(影響力)
書目名稱Recombinant Protein Production in Yeast影響因子(影響力)學(xué)科排名
書目名稱Recombinant Protein Production in Yeast網(wǎng)絡(luò)公開度
書目名稱Recombinant Protein Production in Yeast網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Recombinant Protein Production in Yeast被引頻次
書目名稱Recombinant Protein Production in Yeast被引頻次學(xué)科排名
書目名稱Recombinant Protein Production in Yeast年度引用
書目名稱Recombinant Protein Production in Yeast年度引用學(xué)科排名
書目名稱Recombinant Protein Production in Yeast讀者反饋
書目名稱Recombinant Protein Production in Yeast讀者反饋學(xué)科排名
作者: 柱廊 時間: 2025-3-21 23:20 作者: Cumulus 時間: 2025-3-22 02:30
Felix Bischoff,Martin Giersberg,Falko Matthes,Tobias Schwalenberg,Sebastian Worch,Gotthard Kunze Pflege DNQP vergeben. Der Entwurf wurde dann im M?rz 2014 auf der Konsensuskonferenz vorgestellt und diskutiert. Zu diesem Zeitpunkt wurde der erste Expertenstandard nach diesem Verfahren in der Fach?ffentlichkeit bereits mit Spannung erwartet, zumal das Thema Mobilit?t für alle Pflegeeinrichtungen作者: 審問 時間: 2025-3-22 06:59
Roland Weis Ziel, detaillierte Angaben zur Behandlung von Wunden und Produkten zur Wundversorgung anzubieten. Vielmehr liegt der Fokus auf den Einschr?nkungen der Betroffenen durch die Wunde, etwa durch die Beeintr?chtigung des Selbstwertgefühls, durch Abh?ngigkeit und Einschr?nkungen im Alltag, durch Ver?nder作者: antecedence 時間: 2025-3-22 12:15
Roslyn M. Bill,Annegret Ulke-Lemée,Stephanie P. Cartwright,Rena Far,Jay Kim,Justin A. MacDonald für Patienten und Bewohner eine bedeutende Rolle spielt. Auch in diesem Kapitel werden zun?chst die Inhalte der 1. Aktualisierung des Expertenstandards Ern?hrungsmanagement zur Sicherung und F?rderung der oralen Ern?hrung in der Pflege des Deutschen Netzwerks für Qualit?tsentwicklung in der Pflege 作者: cumber 時間: 2025-3-22 14:45 作者: Anthropoid 時間: 2025-3-22 18:00
Recombinant Protein Production in Yeast978-1-4939-9024-5Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: temperate 時間: 2025-3-22 22:56
Methods in Molecular Biologyhttp://image.papertrans.cn/r/image/824101.jpg作者: podiatrist 時間: 2025-3-23 01:25 作者: compel 時間: 2025-3-23 08:01 作者: certain 時間: 2025-3-23 09:52 作者: 評論者 時間: 2025-3-23 17:46
Tailoring N-Glycan Biosynthesis for Production of Therapeutic Proteins in ,,erythropoietin. The focus of this chapter is to describe the road from yeast-type N-glycosylation to human-type complex N-glycosylation. The chapter describes the cell engineering and provides the detailed analytical procedures required to perform glycan analysis using MALDI-TOF mass spectrometry.作者: 赤字 時間: 2025-3-23 21:18
Guidelines for Small-Scale Production and Purification of Hepatitis B Surface Antigen Virus-Like Pa other areas of biotechnology. Here, we describe in detail simple small-scale production and purification procedures for the generation of hepatitis B surface antigen (HBsAg) VLPs using . as expression host. This protocol may also be applicable with variations to other HBsAg-based VLPs additionally carrying antigens of other pathogens.作者: 不利 時間: 2025-3-24 01:26 作者: cavity 時間: 2025-3-24 05:39 作者: ostrish 時間: 2025-3-24 08:22
Aysun Türkano?lu ?z?elik,Semiramis Y?lmaz,Mehmet Inang gering sind, eine Wirksamkeit konnte jedoch nicht nachgewiesen werden. Der erweiterte Qualit?tsausschuss Pflege hat deshalb im Februar 2018 die freiwillige Einführung des Expertenstandards Mobilit?t für zun?chst zwei Jahre beschlossen. Parallel soll eine Aktualisierung des Expertenstandards und ei作者: medium 時間: 2025-3-24 14:24 作者: 遷移 時間: 2025-3-24 17:06 作者: 死亡率 時間: 2025-3-24 21:19
Roslyn M. Bill,Annegret Ulke-Lemée,Stephanie P. Cartwright,Rena Far,Jay Kim,Justin A. MacDonaldgung. Notwendige Formulare, beispielsweise Vorschl?ge für ein geeignetes Assessment, ein Risikoformular für die Pflegeanamnese, ein Ern?hrungsplan, ein Ern?hrungsprotokoll oder ein Formular für die Bilanzierung, werden beschrieben oder im Anhang vorgestellt.作者: Clumsy 時間: 2025-3-25 02:34 作者: 延期 時間: 2025-3-25 06:47
Promoters, a constitutive promoter. Recent literature showed that newly identified promoters of . are promising as well, in addition to pAOX1 and pGAP..In this chapter, the regulation mechanisms of inducible pAOX1 and constitutive pGAP promoters are discussed. In addition, here we present an overview about th作者: cluster 時間: 2025-3-25 10:49
CRISPR/Cas9-Mediated Homology-Directed Genome Editing in , including simultaneous overexpression of multiple genes. To meet this challenge, we have developed a CRISPR/Cas9-based kit for gene insertions, deletions, and replacements, which paves the way for precise genomic modifications in .. In this chapter, the versatile method for performing these modific作者: 光亮 時間: 2025-3-25 14:08 作者: 抱怨 時間: 2025-3-25 19:04 作者: Brain-Imaging 時間: 2025-3-25 23:58
Yeast Surface Display and Cell Sorting of Antigen-Binding Fc Fragments,ding to multiple antigen molecules, require modifications of traditional affinity maturation strategies. Preferred to equilibrium selections are kinetically driven antigen selections, designed to specifically influence the binding off-rate, which in many cases augments the desired biological effect.作者: 全部逛商店 時間: 2025-3-26 02:18
Efficient Development of a Mixed Feed Process for ,,ulation and guarantee highest productivity. Here, we present an efficient strategy comprising only one experiment to determine the settings of such a mixed feed system based on the physiology of the respective yeast strain.作者: Substance-Abuse 時間: 2025-3-26 07:39
1064-3745 and Protocols.?aims to provide state of the art background and methods for protein producing yeast platforms, as well as case studies for special applications.?.978-1-4939-9024-5Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Dna262 時間: 2025-3-26 08:44
Burcu Gündüz Ergün,Damla Hücceto?ullar?,Sibel ?ztürk,Eda ?elik,P?nar ?al?k作者: promote 時間: 2025-3-26 16:35 作者: 合適 時間: 2025-3-26 17:33 作者: Monolithic 時間: 2025-3-26 22:25
Thomas Gassler,Lina Heistinger,Diethard Mattanovich,Brigitte Gasser,Roland Prielhofer作者: 玷污 時間: 2025-3-27 05:10
Mari A. Piirainen,Heidi Salminen,Alexander D. Frey作者: keloid 時間: 2025-3-27 06:30
Anita Emmerstorfer-Augustin,Tamara Wriessnegger,Melanie Hirz,Guenther Zellnig,Harald Pichler作者: 健談 時間: 2025-3-27 13:16 作者: VOC 時間: 2025-3-27 15:12 作者: 認為 時間: 2025-3-27 20:45
Screening and Selection of Production Strains: Secretory Protein Expression and Analysis in ,itations, such as over-glycosylation and proteolytic degradation, can be encountered in the development of production strains for secretory proteins. Here, . genetic tools and host strains, developed for authentic processing and modification of secretory recombinant proteins, are introduced with the analytical protocols.作者: Suppository 時間: 2025-3-28 01:51
Book 2019bleshooting and avoiding known pitfalls..Authoritative and cutting-edge,?.Recombinant Protein Production in Yeast:?Methods and Protocols.?aims to provide state of the art background and methods for protein producing yeast platforms, as well as case studies for special applications.?.作者: 面包屑 時間: 2025-3-28 03:52 作者: enfeeble 時間: 2025-3-28 06:14
Selection of Heterologous Protein-Producing Strains in ,Here, we present the Golden Gate cloning strategy for the construction of multiple expression cassettes, the transformation into ., and the selection of efficient enzyme-producing strains using an insect alpha-amylase as a reporter detected via a?thermal cycler-based microassay.作者: 和諧 時間: 2025-3-28 14:18
Assessing the Quality of Recombinant Products Made in Yeast,determination of posttranslational modifications such as glycan analysis are not described. In this regard, chromatographic, high-resolution technologies for the integrity of proteins as well as Western blot with specific detection methods are introduced, and individual strengths and perceived limitations are highlighted.作者: 自制 時間: 2025-3-28 17:08
1064-3745 tion advice from the experts.This volume provides an overview of the main yeast production platforms currently used and future yeast cell factories for recombinant protein production. Chapters detail approaches of genetic and metabolic engineering, co-factor containing proteins and virus-like partic作者: HIKE 時間: 2025-3-28 19:33
Established and Upcoming Yeast Expression Systems,rical use, vast amount of data, and experience paved the way for . as a first yeast cell factory, and still it is an important expression platform as being the production host for several large volume products. Continuing special needs of each targeted product and different requirements of bioproces作者: Gerontology 時間: 2025-3-29 02:13 作者: –FER 時間: 2025-3-29 06:25 作者: 安定 時間: 2025-3-29 08:27
Selection of the Optimal Yeast Host for the Synthesis of Recombinant Enzymes,ndatory to obtain full functionality. The wide-range transformation/expression platform presented in this chapter can be used to select the optimal yeast host for high-level synthesis of the desired enzyme with favorable biochemical properties. This platform is composed of a selection marker and up 作者: 有害 時間: 2025-3-29 15:07
Screening and Selection of Production Strains: Secretory Protein Expression and Analysis in ,rapeutic proteins. Despite favorable characteristics of the .-based platform for application to heterologous gene expression, several problems and limitations, such as over-glycosylation and proteolytic degradation, can be encountered in the development of production strains for secretory proteins. 作者: 商店街 時間: 2025-3-29 18:23
Selection of Heterologous Protein-Producing Strains in ,ted for this species have been developed, ranging from the simple cloning of expression vectors to recently developed high-throughput methodologies using efficient cloning and assembly such as Gateway and Golden Gate strategies. The latter strategies, due to their modular character, enable multiple 作者: AVERT 時間: 2025-3-29 22:21
High-Throughput Screening and Selection of , Strains,uction capabilities for all assessed transformants. As clonal variation in such experiments is caused by diverging numbers and possibly also genomic locations of integrated (linearized) expression constructs, the productivity assessment of a larger number of strains is mandatory for selecting a set 作者: facetious 時間: 2025-3-30 02:06
CRISPR/Cas9-Mediated Homology-Directed Genome Editing in , deletion of host genes. For efficient gene deletion, methods such as the split-marker technique have been established. However, synthetic biology trends move toward building up large and complex reaction networks, which often require endogenous gene knockouts and simultaneous overexpression of indi作者: 嘲弄 時間: 2025-3-30 06:28 作者: 野蠻 時間: 2025-3-30 10:24 作者: scrutiny 時間: 2025-3-30 15:11 作者: Indurate 時間: 2025-3-30 17:53 作者: Accrue 時間: 2025-3-30 21:40
Guidelines for Small-Scale Production and Purification of Hepatitis B Surface Antigen Virus-Like Pa other areas of biotechnology. Here, we describe in detail simple small-scale production and purification procedures for the generation of hepatitis B surface antigen (HBsAg) VLPs using . as expression host. This protocol may also be applicable with variations to other HBsAg-based VLPs additionally 作者: 無孔 時間: 2025-3-31 03:35 作者: inchoate 時間: 2025-3-31 05:06
Primary Recovery of Yeast Culture Supernatant for Recombinant Protein Purification,ed. The process consists of a centrifugation step and a depth filtration, followed by a (sterile) membrane filtration. The procedure yields supernatant ready for the next purification step such as chromatography.作者: Cholesterol 時間: 2025-3-31 10:55
Purification of Recombinant Glycoproteins from , Culture Supernatants,ed efficiently by only a few conventional chromatography steps, the purification of glycosylated recombinant proteins is a very challenging process due to the intrinsic feature of the yeast of hypermannosylation. The resulting vast glycosylation pattern on the recombinant target protein masks its ph作者: 他一致 時間: 2025-3-31 16:35 作者: 織物 時間: 2025-3-31 20:51 作者: 殺人 時間: 2025-4-1 00:32
Detection and Elimination of Cellular Bottlenecks in Protein-Producing Yeasts,ein production. Special focus is given to the metabolic impact of protein production. Furthermore, strategies that were shown to enhance secretion of recombinant proteins in different yeast species are presented.作者: 閃光你我 時間: 2025-4-1 03:15 作者: Ablation 時間: 2025-4-1 06:14
Purification of Recombinant Glycoproteins from , Culture Supernatants,ysicochemical properties hampering a conventional downstream process. Here, we describe a fast and efficient two-step chromatography strategy, where both steps are operated in flow-through mode, to purify recombinant glycoproteins from . culture supernatants.
