標(biāo)題: Titlebook: Recombinant Protein Expression in Mammalian Cells; Methods and Protocol David L. Hacker Book 2018 Springer Science+Business Media, LLC, par [打印本頁(yè)] 作者: 我沒有辱罵 時(shí)間: 2025-3-21 19:19
書目名稱Recombinant Protein Expression in Mammalian Cells影響因子(影響力)
書目名稱Recombinant Protein Expression in Mammalian Cells影響因子(影響力)學(xué)科排名
書目名稱Recombinant Protein Expression in Mammalian Cells網(wǎng)絡(luò)公開度
書目名稱Recombinant Protein Expression in Mammalian Cells網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Recombinant Protein Expression in Mammalian Cells被引頻次
書目名稱Recombinant Protein Expression in Mammalian Cells被引頻次學(xué)科排名
書目名稱Recombinant Protein Expression in Mammalian Cells年度引用
書目名稱Recombinant Protein Expression in Mammalian Cells年度引用學(xué)科排名
書目名稱Recombinant Protein Expression in Mammalian Cells讀者反饋
書目名稱Recombinant Protein Expression in Mammalian Cells讀者反饋學(xué)科排名
作者: 招募 時(shí)間: 2025-3-22 00:16
Bench-Scale Stirred-Tank Bioreactor for Recombinant Protein Production in Chinese Hamster Ovary (CHse hamster ovary (CHO-DG44) cells. These operations include bioreactor setup and configuration, batching media, inoculation of the seed cell culture, production phase, and harvest of cell-free fluids.作者: armistice 時(shí)間: 2025-3-22 04:02 作者: 高貴領(lǐng)導(dǎo) 時(shí)間: 2025-3-22 06:37
Scalable Production and Purification of Adeno-Associated Viral Vectors (AAV),ss, cell cultures are further processed using different methods for lysis and recovery. Methods for the purification of viral particles are described, including iodixanol gradient purification, immunoaffinity chromatography, and ultrafiltration, as well as quantitative PCR to quantify vector titer.作者: 放棄 時(shí)間: 2025-3-22 09:55 作者: Frenetic 時(shí)間: 2025-3-22 15:08 作者: isotope 時(shí)間: 2025-3-22 17:12 作者: Legion 時(shí)間: 2025-3-22 23:19 作者: itinerary 時(shí)間: 2025-3-23 02:42 作者: FLASK 時(shí)間: 2025-3-23 06:52 作者: Jacket 時(shí)間: 2025-3-23 12:37 作者: 在前面 時(shí)間: 2025-3-23 15:16 作者: 不滿分子 時(shí)間: 2025-3-23 19:13 作者: 場(chǎng)所 時(shí)間: 2025-3-23 23:19 作者: 拍翅 時(shí)間: 2025-3-24 02:27 作者: FACT 時(shí)間: 2025-3-24 07:13
CHO and HEK293 Cultivation and Transfection in Single-Use Orbitally Shaken Bioreactors,roteins. In this chapter the suspension cultivation and transfection of these cells in small-scale disposable bioreactors is described. The TubeSpin bioreactor 50 and TubeSpin bioreactor 600 are designed for the cultivation of suspension cells by orbital shaking and have maximum working volumes of a作者: 古老 時(shí)間: 2025-3-24 11:00
Bench-Scale Stirred-Tank Bioreactor for Recombinant Protein Production in Chinese Hamster Ovary (CHvariety of different scales ranging from bench to production scales, with working volumes from 10?mL to 25,000?L. Bench-scale STRs are commonly used to culture mammalian cells for process development, to troubleshoot production scale bioreactors using scale-down models (SDM), or to conduct fundament作者: 同義聯(lián)想法 時(shí)間: 2025-3-24 16:17
Continuous and Integrated Expression and Purification of Recombinant Antibodies,a perfusion bioreactor is discussed containing the preparation procedures, the seeding train and the preparation and control of a long-term production run. The downstream processes exploit the benefits of countercurrent chromatography. Their design from batch experiments is presented. The CaptureSMB作者: 積云 時(shí)間: 2025-3-24 20:50
High Throughput Transfection of HEK293 Cells for Transient Protein Production,drug development. Here, we describe a method for high throughput transient transfection of Human Embryonic Kidney 293 (HEK293) cells in 30?mL tubespins using polyethylenimine (PEI) as a transfection reagent. An automated liquid handler can be used to perform pipetting steps for transfecting batches 作者: 滴注 時(shí)間: 2025-3-25 01:39 作者: duplicate 時(shí)間: 2025-3-25 04:21
Genome-Wide High-Throughput RNAi Screening for Identification of Genes Involved in Protein Productie had a profound impact in many areas of basic and applied research. Many groups, both academic and industrial, have been focusing on developing cost-effective methods to improve the production of mammalian proteins that would support potential therapeutic applications. As it stands, while a wide ra作者: 恩惠 時(shí)間: 2025-3-25 07:44 作者: 淘氣 時(shí)間: 2025-3-25 14:48
Application of the CRISPR/Cas9 Gene Editing Method for Modulating Antibody Fucosylation in CHO Cellove both the productivity of recombinant cell lines as well as the quality of therapeutic antibodies. Antibody glycosylation is a critical quality attribute for therapeutic biologics because the glycan patterns on the antibody fragment crystallizable (Fc) region can alter its clinical efficacy and s作者: 產(chǎn)生 時(shí)間: 2025-3-25 17:13
Scalable Production and Purification of Adeno-Associated Viral Vectors (AAV),um in orbital shaken bioreactors and the subsequent purification of vector particles. The protocol for expression of AAV components is based on polyethyleneimine (PEI) mediated transfection of a 2-plasmid system and is specified for production in milliliter to liter scales. After PEI and plasmid DNA作者: 線 時(shí)間: 2025-3-25 22:56
Considerations in the Use of Codon Optimization for Recombinant Protein Expression,e (1) degeneracy of the genetic code enables most amino acids to be encoded by multiple codons and (2) different mRNAs encoding the same protein can vary dramatically in the amount of protein expressed. However, because codon optimization potentially disrupts overlapping information encoded in mRNA 作者: 變異 時(shí)間: 2025-3-26 01:12
Versatile Cell-Free Protein Synthesis Systems Based on Chinese Hamster Ovary Cells,ered mammalian cell lines, this protocol describes the preparation and principle of cell-free protein synthesis systems based on CHO cell lysates. The CHO cell-free system contains endogenous microsomes derived from the endoplasmic reticulum, which enables a direct integration of membrane proteins i作者: 彩色的蠟筆 時(shí)間: 2025-3-26 05:50 作者: 媒介 時(shí)間: 2025-3-26 09:22
PEI-Mediated Transient Gene Expression in CHO Cells,ng pDNA, 2.7?mg/L of nonspecific (filler) DNA and 15?mg/L of PEI. The production phase is performed at 31?°C in the presence of 0.25% .,.-dimethylacetamide (DMA). We also provide information on culture vessel options, recommended working volumes, and recommended shaking speeds for transfections at scales from 2?mL to 2?L.作者: Torrid 時(shí)間: 2025-3-26 14:48 作者: anesthesia 時(shí)間: 2025-3-26 18:19
Recombinant CHO Cell Pool Generation Using piggyBac Transposon System,(PEI). This is followed by a genetic selection for the generation of a cell pool. The resulting cell pool can be used to start a batch or fed-batch culture. Alternatively it can be used for generation of clonal cell lines or generation of cell banks for future use.作者: SAGE 時(shí)間: 2025-3-26 21:50 作者: Occupation 時(shí)間: 2025-3-27 03:06
Continuous and Integrated Expression and Purification of Recombinant Antibodies, process is introduced for continuous capturing while for polishing applications the design of the two-column MCSGP process is described. The chapter also puts these processes together in the context of their integration to an end-to-end production process.作者: 撤退 時(shí)間: 2025-3-27 07:50 作者: 脫水 時(shí)間: 2025-3-27 11:22 作者: Blood-Vessels 時(shí)間: 2025-3-27 15:52
Transient Gene Expression in Suspension HEK293-EBNA1 Cells, chapter, we describe a simple and robust transfection process of suspension-growing human embryo kidney 293 cells using two commercially available serum-free media and polyethylenimine as the transfection reagent. This chapter provides examples for the production and purification of a his-tagged recombinant protein and two monoclonal antibodies.作者: 并排上下 時(shí)間: 2025-3-27 20:22
Stable Expression by Lentiviral Transduction of Cells,and long term producing cells are established. Here, we describe the current state of the art and give details for lab scale production of lentiviral vectors as well as for infection and titration of the viral vectors.作者: 假裝是我 時(shí)間: 2025-3-28 00:52
978-1-4939-9377-2Springer Science+Business Media, LLC, part of Springer Nature 2018作者: 發(fā)生 時(shí)間: 2025-3-28 03:42 作者: languid 時(shí)間: 2025-3-28 08:03 作者: 不規(guī)則 時(shí)間: 2025-3-28 10:56 作者: legislate 時(shí)間: 2025-3-28 14:42 作者: 珍奇 時(shí)間: 2025-3-28 19:06 作者: 稀釋前 時(shí)間: 2025-3-29 01:12 作者: Density 時(shí)間: 2025-3-29 05:29
Yashas RajendraChapter 9, there are many ways to create a Web Forms interface, and the one I’ve created here is just one option among many..The key is that the business objects automatically enforce all business rules and provide business processing so that the UI doesn’t need to include any of that code. As you c作者: 貪婪性 時(shí)間: 2025-3-29 09:33 作者: 杠桿支點(diǎn) 時(shí)間: 2025-3-29 14:42
Iacovos P. Michael,Andras NagyChapter 9, there are many ways to create a Web Forms interface, and the one I’ve created here is just one option among many..The key is that the business objects automatically enforce all business rules and provide business processing so that the UI doesn’t need to include any of that code. As you c作者: GAVEL 時(shí)間: 2025-3-29 18:17 作者: 寬容 時(shí)間: 2025-3-29 23:03 作者: 破裂 時(shí)間: 2025-3-30 03:36
David I. FisherChapter 9, there are many ways to create a Web Forms interface, and the one I’ve created here is just one option among many..The key is that the business objects automatically enforce all business rules and provide business processing so that the UI doesn’t need to include any of that code. As you c作者: 主動(dòng) 時(shí)間: 2025-3-30 05:43
David L. Hacker,Laurence Durrer,Soraya Quincheth the server-side data portal components:.It is also possible to create your own custom network channel by implementing Data Portal Client. I Data Portal Proxy on the client, and Server. I Data Portal Server on the server, just as was done in Chapter 4 to create the four proxy/host combinations lis作者: 不理會(huì) 時(shí)間: 2025-3-30 09:24 作者: 正式通知 時(shí)間: 2025-3-30 12:54
Sarah Inwood,Michael J. Betenbaugh,Madhu Lal,Joseph Shiloach Tr?gerschaften, repr?sentiert durch Volksvertreterinnen und -vertreter ohne zwingenden Professionsbezug, sind dabei als oberstes Führungsorgan bestehen geblieben. In der Umsetzung der neuen Rahmenbedingungen entsteht nun bei den Führungsverantwortlichen ein Orientierungsbedarf, der bisher oft nicht作者: incite 時(shí)間: 2025-3-30 17:22
Kevin Kellner,Ankur Solanki,Thomas Amann,Nga Lao,Niall Barrong und Steuerung von Bildungs- bzw. Lernprozessen an Hochschulen ist eine hochkomplexe Angelegenheit, nicht zuletzt, weil viele verschiedene Anspruchsgruppen ihre Erwartung gegenüber der Hochschule formulieren und Trends wie z.?B. die Digitalisierung oder die Internationalisierung das ??kosystem Bild作者: Haphazard 時(shí)間: 2025-3-30 22:44 作者: 過于光澤 時(shí)間: 2025-3-31 04:34 作者: 負(fù)擔(dān) 時(shí)間: 2025-3-31 07:44
1064-3745 t Protein Expression in Mammalian Cells: Methods and Protocols. aims to aid researchers in building on our knowledge of protein structure and function and to speed the discovery of new therapeutic proteins..978-1-4939-9377-2978-1-4939-8730-6Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 暫停,間歇 時(shí)間: 2025-3-31 10:22
Genome Engineering of Hybridomas to Generate Stable Cell Lines for Antibody Expression,ge; subsequently, the variable heavy (VH) chain is replaced by a fluorescent reporter gene (mRuby) by Cas9-assisted homology-directed repair (HDR). This cell line is customized by replacing mRuby with a synthetic antibody (consisting of a VL, light constant region and VH) by once again using Cas9-as作者: 松馳 時(shí)間: 2025-3-31 15:20 作者: etidronate 時(shí)間: 2025-3-31 18:42 作者: 改正 時(shí)間: 2025-3-31 22:08 作者: neologism 時(shí)間: 2025-4-1 05:39 作者: 內(nèi)向者 時(shí)間: 2025-4-1 08:34 作者: BRAWL 時(shí)間: 2025-4-1 12:35
Iacovos P. Michael,Andras Nagyse security by running the data portal server components on a separate application server. In either case, all you need to do is change some settings in Web.config; the UI code and business objects will work in all these scenarios..In Chapter 11, I’ll show how you can create another type of interfac作者: 乞丐 時(shí)間: 2025-4-1 14:54
Sowmya Balasubramanianse security by running the data portal server components on a separate application server. In either case, all you need to do is change some settings in Web.config; the UI code and business objects will work in all these scenarios..In Chapter 11, I’ll show how you can create another type of interfac
