標(biāo)題: Titlebook: Receptor Tyrosine Kinases; Methods and Protocol Serena Germano Book 2015 Springer Science+Business Media New York 2015 Aberrant expression. [打印本頁] 作者: JAZZ 時(shí)間: 2025-3-21 18:59
書目名稱Receptor Tyrosine Kinases影響因子(影響力)
作者: considerable 時(shí)間: 2025-3-21 23:12
XiangDong Le,Andrew T. Huang,Yunyun Chen,Stephen Y. Lai M.D., Ph.D.作者: Uncultured 時(shí)間: 2025-3-22 00:59
Liang Feng,Wei Wang,Hang-Ping Yao,Jianwei Zhou,Ruiwen Zhang,Ming-Hai Wang作者: 挑剔為人 時(shí)間: 2025-3-22 07:42 作者: thalamus 時(shí)間: 2025-3-22 09:09 作者: brother 時(shí)間: 2025-3-22 13:04
Mangelern?hrung vorbeugen. .Au?erdem:. Beispiele für erforderliche Formulare (z.B. Assessments) und viele?Tipps für den organisatorischen Bereich (z.B. strukturelle Voraussetzungen, Fortbildungen..978-3-642-29993-3作者: micturition 時(shí)間: 2025-3-22 19:58 作者: 圍裙 時(shí)間: 2025-3-22 21:47 作者: 整理 時(shí)間: 2025-3-23 01:36
Noriaki Shimokawa,Noriyuki Koibuchimulare (z.B. Assessment) und viele?Tipps für den organisatorischen Bereich (z.B. strukturelle Voraussetzungen, Fortbildungen..Eine echte Gebrauchsanleitung: kurz und?knapp, verst?ndlich und praktikabel..978-3-642-01323-2作者: 暗語 時(shí)間: 2025-3-23 09:27
1064-3745 and widely used methods employed in this field, providing detailed protocols that molecular and cellular biologists could easily adopt in their research programs on RTKs..978-1-4939-5449-0978-1-4939-1789-1Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Isometric 時(shí)間: 2025-3-23 12:55
Studying N-Linked Glycosylation of Receptor Tyrosine Kinasese beads, which enable precipitation of specifically glycosylated proteins. In this chapter, we describe in detail how to perform pull-down experiments with commercially available lectins to identify changes in the glycosylation of RTKs.作者: 協(xié)迫 時(shí)間: 2025-3-23 17:28
Wirtschaft auf der einen Seite und jenes der liberalen M?rkte auf der anderen Seite zu Kontroversen geführt. Beide Modelle wurden über Epochen hinweg immer wieder politisch und ?konomisch analysiert und fortentwickelt. Aus rechtlicher Sicht stellt sich dabei die Frage, ob und inwieweit solch polit?k作者: 煩躁的女人 時(shí)間: 2025-3-23 18:35 作者: 粗糙濫制 時(shí)間: 2025-3-23 22:42 作者: insular 時(shí)間: 2025-3-24 03:34 作者: 長矛 時(shí)間: 2025-3-24 07:45
Inhee Chung,Ira Mellman aus verschiedenen politischen Lagern. Bei der Beg- achtung des Standes der politischen und publizistischen aber auch der politikwissenscha- lichen Diskussion f?llt jedoch auf, dass viele Einsch?tzungen empirisch kaum fundiert sind. In diesem Forschungsfeld hat die essayistische Vermutung Konjunktur作者: Aggressive 時(shí)間: 2025-3-24 11:09 作者: CHOKE 時(shí)間: 2025-3-24 17:35 作者: 山頂可休息 時(shí)間: 2025-3-24 23:05
Lijuan He,Kalina Hristovagung. Notwendige Formulare, beispielsweise Vorschl?ge für ein geeignetes Assessment, ein Risikoformular für die Pflegeanamnese, ein Ern?hrungsplan, ein Ern?hrungsprotokoll oder ein Formular für die Bilanzierung, werden beschrieben oder im Anhang vorgestellt.作者: 樹膠 時(shí)間: 2025-3-25 02:49
gung. Notwendige Formulare, beispielsweise Vorschl?ge für ein geeignetes Assessment, ein Risikoformular für die Pflegeanamnese, ein Ern?hrungsplan, ein Ern?hrungsprotokoll oder ein Formular für die Bilanzierung, werden beschrieben oder im Anhang vorgestellt.作者: 漸強(qiáng) 時(shí)間: 2025-3-25 04:24
Analysis of Receptor Tyrosine Kinase (RTK) Phosphorylation by Immunoblottingdecades. Despite the development of quantitative mass spectrometry, reverse-phase protein array, and multiplex technologies, immunoblotting with phospho-specific antibodies is still used in parallel with these technologies and remains a powerful, and reproducible, method for interrogating signaling networks involving RTKs.作者: macular-edema 時(shí)間: 2025-3-25 09:23
Quantification of the Effects of Mutations on Receptor Tyrosine Kinase (RTK) Activation in Mammalianotocol for the quantification of the effect of mutations on RTK activation in mammalian cells. The activation measurements are based on Western blotting, and involve direct comparison of receptor phosphorylation under conditions that ensure identical expression of wild-type and mutant receptors.作者: Contend 時(shí)間: 2025-3-25 14:17
Downregulation of Receptor Tyrosine Kinases Through Ubiquitination: Analysis by Immunodetectionuate signal transduction. Ubiquitination is a critical step involved in the downregulation of RTK signaling. Here, we describe how to immunodetect the ligand-induced ubiquitination and degradation of TrkA, an RTK, by immunoprecipitation and Western blotting.作者: GROWL 時(shí)間: 2025-3-25 17:42 作者: Highbrow 時(shí)間: 2025-3-25 23:56
https://doi.org/10.1007/978-1-4939-1789-1Aberrant expression; Negative regulation; Pharmacological targets; Protein expression; RTK activation; RT作者: 轉(zhuǎn)換 時(shí)間: 2025-3-26 03:41 作者: commensurate 時(shí)間: 2025-3-26 06:08 作者: 歪曲道理 時(shí)間: 2025-3-26 11:42 作者: 包庇 時(shí)間: 2025-3-26 16:27
Analysis of Changes in Phosphorylation of Receptor Tyrosine Kinases: Antibody Arraysays that are basically used to analyse phosphorylation and dephosphorylation of RTKs. Antibody arrays include well-characterized antibodies for profiling, changes in RTK expression, and comparison between normal, diseased, or treated samples.作者: 植物群 時(shí)間: 2025-3-26 19:36 作者: 惰性女人 時(shí)間: 2025-3-26 22:49
Analysis of Receptor Tyrosine Kinase Gene Amplification on the Example of , the cell membranes for hybridization, so that the probe can bind to the complementary DNA target. Posthybridization includes washing steps to remove excessive probes and detection of probes via secondary marked fluorochromes. DAPI stains nuclei and serves as mounting media.作者: endarterectomy 時(shí)間: 2025-3-27 01:41 作者: 態(tài)學(xué) 時(shí)間: 2025-3-27 05:22 作者: diskitis 時(shí)間: 2025-3-27 09:50
1064-3745 s and implementation advice from the experts.Receptor Tyrosine Kinases: Methods and Protocols. explores on the most widely studied of the protein classes, known for their central role in several cellular functions and in a variety of human pathologies. Beginning with a section on activation and sign作者: 衣服 時(shí)間: 2025-3-27 15:04
Analysis of Receptor Tyrosine Kinase (RTK) Phosphorylation by Immunoblottingdecades. Despite the development of quantitative mass spectrometry, reverse-phase protein array, and multiplex technologies, immunoblotting with phospho-specific antibodies is still used in parallel with these technologies and remains a powerful, and reproducible, method for interrogating signaling 作者: Creatinine-Test 時(shí)間: 2025-3-27 18:31
Analysis of Changes in Phosphorylation of Receptor Tyrosine Kinases: Antibody Arraysransmembrane ligand-binding proteins are important mediators of the signaling cascade and includes EGFR, PDGFR (platelet-derived growth factor receptors), FGFR (fibroblast growth factor receptor) and the IR (insulin receptor). RTKs comprise 59 members and their structure includes an extracellular li作者: Kernel 時(shí)間: 2025-3-27 22:34 作者: 氣候 時(shí)間: 2025-3-28 03:23 作者: 好色 時(shí)間: 2025-3-28 07:34
Evaluation of the Dimerization Profiles of HER Tyrosine Kinases by Time-Resolved F?rster Resonance Eeceptor dimerization, which is a crucial step for cellular signal transduction and diversification. The HER family includes four members (EGFR/HER1, HER2, HER3, and HER4) that can homodimerize or heterodimerize. Here, we describe immunoassays based on time-resolved F?rster resonance energy transfer 作者: ASSAY 時(shí)間: 2025-3-28 10:52 作者: abduction 時(shí)間: 2025-3-28 14:45
Analysis of Receptor Tyrosine Kinase Gene Amplification on the Example of , aberrations (deletions and amplifications) and structural aberrations (translocations and fusions) are detectable. Probes bind complementary to the DNA strand of the region of interest. Subsequently, the probes are detected via fluorochromes and appear as colored dots that can be assessed under the作者: Exhilarate 時(shí)間: 2025-3-28 21:39
Quantification of the Effects of Mutations on Receptor Tyrosine Kinase (RTK) Activation in Mammalianotocol for the quantification of the effect of mutations on RTK activation in mammalian cells. The activation measurements are based on Western blotting, and involve direct comparison of receptor phosphorylation under conditions that ensure identical expression of wild-type and mutant receptors.作者: 摻和 時(shí)間: 2025-3-28 23:34 作者: 斜坡 時(shí)間: 2025-3-29 06:10
Studying N-Linked Glycosylation of Receptor Tyrosine Kinasessecondary pathway to glycolysis, hexosamine biosynthetic pathway (HBP). HBP provides substrate for N-linked glycosylation, which occurs in the endoplasmic reticulum and the Golgi apparatus. N-linked glycosylation supports protein folding and correct sorting of proteins to plasma membrane and secreti作者: 外來 時(shí)間: 2025-3-29 10:10 作者: 擁護(hù) 時(shí)間: 2025-3-29 11:40
Downregulation of Receptor Tyrosine Kinases Through Ubiquitination: Analysis by Immunodetectionuate signal transduction. Ubiquitination is a critical step involved in the downregulation of RTK signaling. Here, we describe how to immunodetect the ligand-induced ubiquitination and degradation of TrkA, an RTK, by immunoprecipitation and Western blotting.作者: coddle 時(shí)間: 2025-3-29 18:33 作者: pancreas 時(shí)間: 2025-3-29 23:22
Human Tumor Xenografts in Mouse as a Model for Evaluating Therapeutic Efficacy of Monoclonal Antibodlogy. Currently, numerous therapeutic monoclonal antibodies are under preclinical development. The potential for moving candidate antibodies into clinical trials relies heavily on therapeutic efficacy validated by human tumor xenografts in mice. Here we describe methods used to determine therapeutic作者: 審問 時(shí)間: 2025-3-30 02:56
Receptor Tyrosine Kinase Targeting in Multicellular Spheroidss becoming increasingly popular. This is due to the understanding that cells grown as spheroids tend to replicate the in vivo situation more reliably than monolayer cells. Thus, the use of multicellular spheroids may be more clinically relevant than monolayer cell cultures. Here, we describe methods作者: Judicious 時(shí)間: 2025-3-30 05:30 作者: ESO 時(shí)間: 2025-3-30 09:13
Cell Surface Biotinylation of Receptor Tyrosine Kinases to Investigate Intracellular Traffickingace into early endosomes and then travel through intracellular compartments to either recycle back to the membrane or degrade in lysosomes. The biotin-labeled proteins may be detected through affinity purification with streptavidin agarose resins. This chapter describes methods for cell surface biotinylation to assess RTK trafficking steps.作者: reject 時(shí)間: 2025-3-30 14:21 作者: Dislocation 時(shí)間: 2025-3-30 20:19
Analysis of Epidermal Growth Factor Receptor Dimerization by BS3 Cross-Linkingmers using BS. cross-linking. BS. cross-linking is well suited for this purpose because of its length, water solubility, and membrane impermeability. Although this protocol is written specifically for EGFR, the assay can be extrapolated in order to characterize dimerization of other receptor tyrosine kinases.作者: 去才蔑視 時(shí)間: 2025-3-30 22:11
Single-Molecule Optical Methods Analyzing Receptor Tyrosine Kinase Activation in Living Cellstal internal reflection fluorescence microscopy (TIRFM), and initial steps of data analysis to identify the time-dependent variation of single-receptor diffusion, which can be widely applied to studying activation of various cell surface receptors.作者: Temporal-Lobe 時(shí)間: 2025-3-31 00:51
Human Tumor Xenografts in Mouse as a Model for Evaluating Therapeutic Efficacy of Monoclonal Antibod efficacy of monoclonal antibodies or antibody-drug conjugates specific to human receptor tyrosine kinase using human tumor xenografts in mice as the model. The end point of the study is to determine whether treatment of tumor-bearing mice with a monoclonal antibody or antibody-drug conjugates results in significant delay of tumor growth.作者: 禁止 時(shí)間: 2025-3-31 05:35 作者: 畢業(yè)典禮 時(shí)間: 2025-3-31 11:03
nvestmentbank ?Lehmann Brothers“ 2008, dem Zusammenbruch der internationalen Finanzsysteme und dem übergreifen der Krise auf die Realwirtschaft wurde eine neue Grundsatzdiskussion über den Umgang mit dem ungezügelten globalen Kapitalismus und die damit verbundene Neuordnung der M?rkte entfacht. [1]
