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標(biāo)題: Titlebook: Receptor Signal Transduction Protocols; Gary B. Willars,R. A. John Challiss Book 20042nd edition Humana Press 2004 [打印本頁]

作者: 習(xí)慣    時間: 2025-3-21 17:16
書目名稱Receptor Signal Transduction Protocols影響因子(影響力)




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書目名稱Receptor Signal Transduction Protocols讀者反饋學(xué)科排名





作者: 半身雕像    時間: 2025-3-21 22:15

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1064-3745 tionally recognized experts in their fields who have many years of experience using the methods they describe. This not only allows each chapter to impart a clear description of the method, but978-1-59259-754-3Series ISSN 1064-3745 Series E-ISSN 1940-6029
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ding to the real ones in terms of internal pressure, temperature, corrosion, etc., which has been of decisive importance for the transferability of the test results to real components. In addition, a series of complementary issues has been investigated (material science aspects, nondestructive exami
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David B. Bylund,Jean D. Deupree,Myron L. Toewsding to the real ones in terms of internal pressure, temperature, corrosion, etc., which has been of decisive importance for the transferability of the test results to real components. In addition, a series of complementary issues has been investigated (material science aspects, nondestructive exami
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Sebastian Lazarenoding to the real ones in terms of internal pressure, temperature, corrosion, etc., which has been of decisive importance for the transferability of the test results to real components. In addition, a series of complementary issues has been investigated (material science aspects, nondestructive exami
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Lucy F. Donaldsonogy in urban planning. This book introduces expert systems for problem solving in urban planning and describes the way in which heuristic knowledge and rules of thumb of expert planners can be represented through computer programs. The book presents practical applications of expert systems for solving many im978-1-4612-7976-1978-1-4612-3348-0
作者: Asymptomatic    時間: 2025-3-24 03:16
Rafael Lujánogy in urban planning. This book introduces expert systems for problem solving in urban planning and describes the way in which heuristic knowledge and rules of thumb of expert planners can be represented through computer programs. The book presents practical applications of expert systems for solving many im978-1-4612-7976-1978-1-4612-3348-0
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Ian Mullaney,Graeme Milligannking about sets of data instead of individual rows. Your querieswill run faster, be easier to write, and easier to deconstruct, maintain, and enhance in the future..Just knowing and using these functions is not enough. You also need to understand how to tune the queries. .Expert T-SQL Window Functi
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Richard J. Ward,Graeme Milligannking about sets of data instead of individual rows. Your querieswill run faster, be easier to write, and easier to deconstruct, maintain, and enhance in the future..Just knowing and using these functions is not enough. You also need to understand how to tune the queries. .Expert T-SQL Window Functi
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Adriano Marchese,Jeffrey L. Benovicct fits into the Twisted ecosystem and what it does, and offers several examples to bring readers up to speed, with pointers to additional resources for more depth. Examples include using Twisted with Docker, as a WSGI container, for file sharing, and more..What You‘ll Learn.Integrate Twisted and as
作者: 繼承人    時間: 2025-3-25 18:45
Marion Blomenr?hr,Henry F. Vischer,Jan Bogerdct fits into the Twisted ecosystem and what it does, and offers several examples to bring readers up to speed, with pointers to additional resources for more depth. Examples include using Twisted with Docker, as a WSGI container, for file sharing, and more..What You‘ll Learn.Integrate Twisted and as
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作者: 粗俗人    時間: 2025-3-26 00:55
Methods in Molecular Biologyhttp://image.papertrans.cn/r/image/823548.jpg
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,Identification and Quantitation of G-Protein α-Subunits,ins) has led to the development of specific techniques that can be used to identify which of these polypeptide(s) is involved on receptor activation by ligand. In addition, these methods can be used to probe the specificity of the interaction and to yield information about the stoichiometries involved.
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Book 20042nd editionr properties per se, and their coupling to immediate downstream binding partners—principally, although not exclusively, the heterotrimeric G-proteins. The new edition combines updates of key chapters from the first edition, as well as a large number of new contributions covering key methodologies th
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作者: 厚顏無恥    時間: 2025-3-27 02:03
Identification of G-Protein-Coupled Receptor mRNA Expression by Northern Blotting and , Hybridizatis the ability to identify genetic splice variants, or to study multiple RNA molecules sequentially in the same tissue samples..These protocols give step-by-step instructions for the performance of these techniques, and the analysis of the data that can be obtained using them.
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Generation of Model Cell Lines Expressing Recombinant G-Protein-Coupled Receptors,ran, cationic lipids, and electroporation), allowing their transient or stable expression in diverse cell lines. This chapter gives a brief overview of the different techniques and provides methodology for the generation of transiently transfected cells and for selection, isolation and maintenance of stable transfected cell lines.
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Radioligand-Binding Methods for Membrane Preparations and Intact Cells,ing assays for G-protein-coupled receptors in membranes and intact cells are presented for these types of experiments. Detailed procedures for analysis of the data obtained from these experiments are also given.
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Radioligand-Binding Methods for Membrane Preparations and Intact Cells,igand-binding experiments: (1) saturation experiments from which the affinity of the radioligand for the receptor and the binding site density can be determined; (2) inhibition experiments from which the affinity of a competing, unlabeled compound for the receptor can be determined; and (3) kinetic
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Immunocytochemical Identification of G-Protein-Coupled Receptor Expression and Localization, expression and localization of proteins both in model systems and their physiological context. This chapter details the theory and practice of the technique as well as lists the materials required. A general protocol is proposed, which can be adapted to suit the needs of individual investigators us
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Identification of G-Protein-Coupled Receptor mRNA Expression by Northern Blotting and , Hybridizati. hybridization (ISH) and Northern blotting are powerful methods for the localization of mRNAs and the study of regulation of mRNA expression. ISH combines the power of precise cellular localization with the ability to perform semiquantitative analysis of the mRNA level, whereas Northern blotting ha
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Generation of Model Cell Lines Expressing Recombinant G-Protein-Coupled Receptors, recombinant systems. In this respect, transfected mammalian cell lines constitute the most frequently used model for investigating the pharmacological and biochemical properties of these receptors. Several protocols have been described (based on the use of calcium phosphate precipitation, DEAE dext
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Covalent Modification of G-Proteins by Affinity Labeling,nsmembrane receptors function as the guanine nucleotide exchange factors (GEFs). A research goal may be to determine which G-protein α-subunit is activated by the receptor under investigation. In a membrane preparation obtained from cells or tissues this can be achieved in a seemingly straightforwar
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,Identification and Quantitation of G-Protein α-Subunits,ins) has led to the development of specific techniques that can be used to identify which of these polypeptide(s) is involved on receptor activation by ligand. In addition, these methods can be used to probe the specificity of the interaction and to yield information about the stoichiometries involv
作者: inspiration    時間: 2025-3-30 14:31
Analysis of Function of Receptor-G-Protein and Receptor-RGS Fusion Proteins,ese signaling systems. Here we describe some of the various techniques and methods used in detail. The process of fusing the two components, the receptor and the G-protein α-subunit, result in a construct that acts as an agonist stimulated GTPase enzyme whose expression level can be accurately deter
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Ubiquitination of G-Protein-Coupled Receptors,R with an epitope-tagged ubiquitin construct in a heterologous expression system. Modification by ubiquitin of the GPCR resulting from agonist activation is detected by immunoprecipation and subsequent immunoblotting for the epitope-tagged ubiquitin. We use here the chemokine receptor CXCR4 as the m
作者: 障礙物    時間: 2025-3-31 11:30
Receptor Mutagenesis Strategies for Examination of Structure-Function Relationships,ted mutagenesis using either the Altered Sites? II in vitro mutagenesis system or the GeneTailor? site-directed mutagenesis system can generate base substitutions/deletions/insertions that yield single/multiple amino acid substitutions/deletions/insertions and/or N- or C-terminal truncations in GPCR
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ent, and of the efforts directed towards the development and application of the related expert systems. The above mentioned activities are mostly related to pressurized nuclear and non-nuclear components. A large part of the research work has been performed within the scope of the Reactor Safety Res
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Emmanuel HermansTENGRUPPE*** Of interest to Urban and Regional planners, civil engineers, geographers; computer scientists; operations researchers; landscape architects; and advanced students in the above disciplines.- Level: Technical Book, Monograph ***URHEBER*** .T.J. Kim,. University of Illinois, Champaign, IL;




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