作者: aptitude 時(shí)間: 2025-3-21 21:34
Anthony P. Davenport,Rhoda E. Kucated the common press to coin the phrase ‘Dr. med. computer‘. Papers printed under this heading rebuffed the majority of physiCians for many years. Today we kno978-3-540-50317-0978-3-642-48706-4Series ISSN 0172-7788 作者: Calculus 時(shí)間: 2025-3-22 04:22
Peter Johnstr?m,Tim D. Fryer,Hugh K. Richards,Olivier Barret,Anthony P. Davenportescribe the prototypes. This material is usually in the form of sample rules and a description of the process for applying the rules. The third location of the 978-94-010-5746-2978-94-011-3923-6Series ISSN 0923-6716 Series E-ISSN 2199-1057 作者: observatory 時(shí)間: 2025-3-22 07:22
escribe the prototypes. This material is usually in the form of sample rules and a description of the process for applying the rules. The third location of the 978-94-010-5746-2978-94-011-3923-6Series ISSN 0923-6716 Series E-ISSN 2199-1057 作者: 規(guī)章 時(shí)間: 2025-3-22 12:20
Receptor Databases and Computational Websites for Ligand Binding,rotein and nucleotide sequences, classification, experimental results, and computational tools for modeling interactions. A number of websites in the public domain provide useful data-mining tools and contain information on specific families of receptors or receptor subfamilies, such as the G protei作者: FORGO 時(shí)間: 2025-3-22 13:14
,Identification of Orphan G Protein-Coupled Receptor Ligands Using FLIPR? Assays,25 billion in worldwide pharmaceutical sales. Therefore, considerable effort has been made to identify cognate ligands for oGPCRs and functionally characterize these receptors in order to elucidate their physiological and therapeutic relevance.作者: Manifest 時(shí)間: 2025-3-22 18:11
mRNA, in pathological states and the possible modes of action of drugs used to treat such conditions (.–.). Changes at the molecular level to alter mRNA expression represent rapid changes within a cell; therefore, it can be envisaged that such studies on human biopsy and post mortem tissue will lead to a作者: 精美食品 時(shí)間: 2025-3-22 21:53
Radioligand-Binding and Molecular-Imaging Techniques for the Quantitative Analysis of Established aes, and has been very successful. More than 45 receptors have been ″de-orphanized″ or paired with their cognate ligand, with nearly half of these putative endo- genous transmitters turning out to be peptides (..). The number of pairings continues to increase. It is estimated that about 70 of the rem作者: fallible 時(shí)間: 2025-3-23 04:45
Live Cell Imaging of G Protein-Coupled Receptors,ffinity, signal transduction, or intracellular trafficking) of GPCRs are not, or are only slightly, altered (.–.). Thus, GFP and its derivatives have been widely applied as fluorescent probes to visualize trafficking of GPCRs in real time, to analyze GPCRs‘ mobility by fluorescence recovery after ph作者: 模范 時(shí)間: 2025-3-23 08:00 作者: Oversee 時(shí)間: 2025-3-23 13:45 作者: 貝雷帽 時(shí)間: 2025-3-23 17:10 作者: paradigm 時(shí)間: 2025-3-23 18:58
Stephen A. Douglas,Zhaohui Ao,Douglas G. Johns,Kristeen Maniscalco,Robert N. Willette,Lea Sarov-Blatl and social implications of these rapid technological developments; referring to what he then called: ‘The Computer and the new decision making techniques ..? ‘ Concerning this last point, there is little need to emphasize that had been less practical application in organizations than the often impressive th978-94-010-8193-1978-94-009-3805-2作者: preservative 時(shí)間: 2025-3-24 01:35 作者: 序曲 時(shí)間: 2025-3-24 06:25
David A. Walsh,John Whartonrge cliques. This is not surprising because as we have seen in Chapter 8, the task of exact propagation has been proven to be .-hard (see Cooper (1990)). Thus, from the practical point of view, exact propagation methods may be restrictive or even inefficient in situations where the type of network s作者: 饑荒 時(shí)間: 2025-3-24 09:05
Anthony P. Davenport,Rhoda E. Kucrge cliques. This is not surprising because as we have seen in Chapter 8, the task of exact propagation has been proven to be .-hard (see Cooper (1990)). Thus, from the practical point of view, exact propagation methods may be restrictive or even inefficient in situations where the type of network s作者: Conquest 時(shí)間: 2025-3-24 14:43
Burkhard Wiesner,Michael Beyermann,Alexander Okscheof assessment criteria for matching schemes is derived. Initial ideas for a generic matching concept using graph matching techniques is proposed. Discussion of the new approach is mainly devoted to control of matching by prediction graphs derived from model data.作者: Insatiable 時(shí)間: 2025-3-24 17:44
Peter Johnstr?m,Anthony P. Davenportearn. There is a rich set of problems that are well-suited to this type of tool. Most of the systems described in this book have been developed with these relatively simple tools..Larger and more complex applications seem to require more sophisticated tools. Frame-based systems provide a growth path作者: 談判 時(shí)間: 2025-3-24 21:38 作者: cauda-equina 時(shí)間: 2025-3-25 00:57 作者: arcane 時(shí)間: 2025-3-25 06:59
Methods in Molecular Biologyhttp://image.papertrans.cn/r/image/823545.jpg作者: 木訥 時(shí)間: 2025-3-25 11:04
https://doi.org/10.1385/1592599273PCR; cell; enzymes; hybridization; ion channels; lipide; microscopy; protein; radiography; research作者: indoctrinate 時(shí)間: 2025-3-25 15:30 作者: Processes 時(shí)間: 2025-3-25 19:10 作者: leniency 時(shí)間: 2025-3-25 20:15 作者: entail 時(shí)間: 2025-3-26 02:38 作者: 不能仁慈 時(shí)間: 2025-3-26 07:06
,Quantitative Analysis of Orphan G Protein-Coupled Receptor mRNAs by TaqMan? Real-Time PCR,oximately one-half of all drugs currently marketed exert their actions, either directly or indirectly, via GPCRs (.). Given the potential commercial opportunities emanating from the identification of small molecule modulators of “novel” GPCRs (currently, GPCRs generate in excess of $25 billion per y作者: musicologist 時(shí)間: 2025-3-26 08:45
mRNA,hern hybridization, first described by Alwine et al. (.), has become a very powerful technique in many research areas, including that of receptor research. The applications of these techniques are many and include (1) direct assessment of the presence, distribution, and modulation under different ph作者: 阻撓 時(shí)間: 2025-3-26 14:25
Radioligand-Binding and Molecular-Imaging Techniques for the Quantitative Analysis of Established alike, seven-transmembrane-spanning G protein-coupled receptors (GPCRs). More than 200 receptors that transduce many important physiological processes and are the target for about 50% of all drugs have been identified in this family (.–.) . A further approx 160 or so ″orphan″ GPCRs have been predicte作者: Diskectomy 時(shí)間: 2025-3-26 18:36
Autoradiography of Enzymes, Second Messenger Systems, and Ion Channels,s have been studied via selective inhibitors, ion channels using naturally occurring toxins, and second messenger systems using inositol polyphosphates. Ligand binding complements immunohistochemistry (..) and . hybridization (..) by permitting pharmacological characterization and quantification of 作者: 清醒 時(shí)間: 2025-3-26 21:09
Immunocytochemical Localization of Receptors Using Light and Confocal Microscopy With Application tated using examples of how site-directed polyclonal antisera can be used to distinguish between subtypes of G protein-coupled receptors (GPCRs; endothelin ET. and ET.), (.) in sections of human tissue or whole-body sections of mice, as well as mapping previously designated orphan receptors (oGPCR) r作者: 柔美流暢 時(shí)間: 2025-3-27 02:31
Live Cell Imaging of G Protein-Coupled Receptors, analysis of the transport of GPCRs was the molecular cloning of the green fluorescent protein (GFP) from the jellyfish . by Prasher and coworkers (., .). Site-directed mutagenesis yielded derivatives with higher photostability, improved quantum yield, and different excitation/emission spectra (.– .作者: 離開真充足 時(shí)間: 2025-3-27 06:31
Imaging and Characterization of Radioligands for Positron Emission Tomography Using Quantitative Phon sensitive film (.) (.. and .). The major advantage of phosphor screens over film is the greatly increased sensitivity with exposure times reduced by at least one order of magnitude (.,.). This is essential for short-lived radionuclides—such as .F and .1C, with half-lives of 109.8 and 20.4 min, re作者: 手術(shù)刀 時(shí)間: 2025-3-27 10:16 作者: Neuropeptides 時(shí)間: 2025-3-27 17:39
Book 2005Latest editionfer, and quantitative analysis of receptor mRNA by TaqMan PCR. These book equips the researcher with techniques for exploring the unprecedented number of new receptor systems now emerging and the so-called "orphan" receptors whose activating ligand has not been identified.作者: 同義聯(lián)想法 時(shí)間: 2025-3-27 18:54
Autoradiography of Enzymes, Second Messenger Systems, and Ion Channels,ome available before molecular and immunological reagents have been developed. A pharmacologically active agent may be synthesized before the endogenous ligand for its binding site has been identified, and autoradiographic methods may help elucidate the site of action of such agents.作者: 柳樹；枯黃 時(shí)間: 2025-3-28 00:28 作者: Haphazard 時(shí)間: 2025-3-28 04:33 作者: 舊石器 時(shí)間: 2025-3-28 06:45 作者: 半導(dǎo)體 時(shí)間: 2025-3-28 13:27 作者: Influx 時(shí)間: 2025-3-28 15:48 作者: CORE 時(shí)間: 2025-3-28 21:03
Dynamic In Vivo Imaging of Receptors in Small Animals Using Positron Emission Tomography,ms such as the dopamine, serotonin, benzodiazepine, opiate, and cholinergic systems (.). Over the years, PET has increasingly been recognized as a very powerful tool to accelerate development and assessment of existing and novel drugs (.–.).作者: Nomadic 時(shí)間: 2025-3-29 00:05 作者: 殘酷的地方 時(shí)間: 2025-3-29 06:54 作者: NIP 時(shí)間: 2025-3-29 08:25 作者: connoisseur 時(shí)間: 2025-3-29 11:36 作者: Geyser 時(shí)間: 2025-3-29 18:38 作者: LIMN 時(shí)間: 2025-3-29 21:02
Anthony P. Davenport,Rhoda E. Kucnference of the European Federation for Medical Informatics and takes place at Hannover, F. R. of Germany, from September 26 to 29, 1988. It was planned and initililly prepared by the late Prof. P. L Reichertz, who headed the Hannover institute from 1969 to 1987. To commemorate his contribution to t作者: 天氣 時(shí)間: 2025-3-30 02:58
Jenny Berry,Molly Price-Jonesf the European Federation for Medical Informatics and takes place at Hannover, F. R. of Germany, from September 26 to 29, 1988. It was planned and initililly prepared by the late Prof. P. L Reichertz, who headed the Hannover institute from 1969 to 1987. To commemorate his contribution to the develop作者: Cultivate 時(shí)間: 2025-3-30 07:25
