標題: Titlebook: Ras Activity and Signaling; Methods and Protocol Ignacio Rubio,Ian Prior Book 2021 Springer Science+Business Media, LLC, part of Springer N [打印本頁] 作者: Magnanimous 時間: 2025-3-21 16:54
書目名稱Ras Activity and Signaling影響因子(影響力)
書目名稱Ras Activity and Signaling影響因子(影響力)學科排名
書目名稱Ras Activity and Signaling網(wǎng)絡公開度
書目名稱Ras Activity and Signaling網(wǎng)絡公開度學科排名
書目名稱Ras Activity and Signaling被引頻次
書目名稱Ras Activity and Signaling被引頻次學科排名
書目名稱Ras Activity and Signaling年度引用
書目名稱Ras Activity and Signaling年度引用學科排名
書目名稱Ras Activity and Signaling讀者反饋
書目名稱Ras Activity and Signaling讀者反饋學科排名
作者: Obstacle 時間: 2025-3-21 22:28 作者: 輕浮思想 時間: 2025-3-22 02:16 作者: Antioxidant 時間: 2025-3-22 06:58 作者: 不容置疑 時間: 2025-3-22 12:08
ed since Volume 3 was published in 1977. There is also a small number of entries giving recently determined structure or addi- tional information regarding alkaloids given in the preceding three volumes. It is a great pleasure to thank the staff of the John Rylands Science Library of the Univer- sit作者: 白楊魚 時間: 2025-3-22 13:03 作者: 俗艷 時間: 2025-3-22 20:07 作者: Glaci冰 時間: 2025-3-23 01:06 作者: Sinus-Node 時間: 2025-3-23 02:25 作者: Hallowed 時間: 2025-3-23 07:30 作者: 訓誡 時間: 2025-3-23 12:00
Kari Kopra,Harri H?rm?ed since Volume 3 was published in 1977. There is also a small number of entries giving recently determined structure or addi- tional information regarding alkaloids given in the preceding three volumes. It is a great pleasure to thank the staff of the John Rylands Science Library of the Univer- sit作者: Iatrogenic 時間: 2025-3-23 17:31
Ryan C. Killoran,Matthew J. Smithed since Volume 3 was published in 1977. There is also a small number of entries giving recently determined structure or addi- tional information regarding alkaloids given in the preceding three volumes. It is a great pleasure to thank the staff of the John Rylands Science Library of the Univer- sit作者: PURG 時間: 2025-3-23 19:50
olume 3 was published in 1977. There is also a small number of entries giving recently determined structure or addi- tional information regarding alkaloids given in the preceding three volumes. It is a great pleasure to thank the staff of the John Rylands Science Library of the Univer- sity of Manch作者: LEVY 時間: 2025-3-24 00:11
Ras Variant Biology and Contributions to Human Disease isoform and mutation variants differentially contribute to these diseases and provide an opportunity for deeper understanding of Ras function. The putative mechanisms underpinning these differences, new approaches that are being applied, and some of the key questions and challenges that remain are 作者: BRAND 時間: 2025-3-24 03:08
Regulation of the Small GTPase Ras and Its Relevance to Human Diseaseed and conditioned by a strongly reinvigorated determination within the Ras community to develop therapeutics attacking . the Ras oncoproteins. As a member of the small G-protein superfamily, function and transforming activity of Ras all revolve about its GDP/GTP loading status. For one thing, the e作者: declamation 時間: 2025-3-24 09:49 作者: SPALL 時間: 2025-3-24 14:41
Absolute Quantitation of GTPase Protein Abundancenal redundancy between closely related family members are commonly observed. Antibody-based methods are commonly used to characterize their protein expression. However, these approaches are typically semi-quantitative, and the requirement to use different antibodies means that this strategy is not s作者: 殺人 時間: 2025-3-24 18:47 作者: 共同生活 時間: 2025-3-24 20:09 作者: 姑姑在炫耀 時間: 2025-3-25 01:01
Active GTPase Pulldown Protocol exists in a transient GTP bound state that mediates downstream signalling events. The dysregulation of these GTPases has been associated with the progression of multiple diseases, most prominently cancer and developmental syndromes known as .. Determining the activation state of Ras and its relativ作者: delusion 時間: 2025-3-25 07:22 作者: curettage 時間: 2025-3-25 10:38 作者: 平靜生活 時間: 2025-3-25 12:46 作者: 紅腫 時間: 2025-3-25 19:49 作者: dry-eye 時間: 2025-3-25 23:38 作者: 內(nèi)向者 時間: 2025-3-26 03:35 作者: 發(fā)炎 時間: 2025-3-26 06:59
Assessment of Plasma Membrane Fatty Acid Composition and Fluidity Using Imaging Flow Cytometryg. Our previous work has demonstrated that plasma membrane fatty acid composition is an important determinant of oncogenic Ras signaling and that dietary (exogenous) modulation of membrane composition may underlie the chemoprotective benefits of long chain n-3 polyunsaturated fatty acids (PUFA). In 作者: 縱火 時間: 2025-3-26 08:55 作者: 瑣碎 時間: 2025-3-26 13:59
1064-3745 ation advice from the experts.This volume compiles new experimental approaches and concepts focusing mostly, but not solely, on ways to manipulate and regulate Ras activity and its downstream signaling output. Chapters detail standard methodologies, biochemical methods, Ras processing trafficking an作者: Mumble 時間: 2025-3-26 18:22 作者: CAGE 時間: 2025-3-26 23:21 作者: 變異 時間: 2025-3-27 02:59
Super-Resolution Imaging and Spatial Analysis of RAS on Intact Plasma Membrane Sheetscluster size, clustered fraction, and population distribution can be obtained by the univariate spatial distribution analysis. Intermolecular associations between different RAS isoforms, RAS and various PM lipids, as well as RAS and diverse effectors can be quantified via bivariate co-localization analysis.作者: indigenous 時間: 2025-3-27 07:48
Book 2021s downstream signaling output. Chapters detail standard methodologies, biochemical methods, Ras processing trafficking and localization, Ras signaling and inhibition, and .in vivo. models for studying Ras function. Written in the highly successful?.Methods in Molecular Biology.?series format, chapte作者: Notify 時間: 2025-3-27 10:54
Validation of Isoform- and Mutation-Specific RAS Antibodiesd for well-validated antibodies to evaluate the function and expression of the different RAS isoforms. Here we describe our experimental approaches to characterize RAS antibodies for their isoform- and mutant-specificity for use in immunoblot analyses.作者: 包租車船 時間: 2025-3-27 16:10
Production and Membrane Binding of N-Terminally Acetylated, C-Terminally Farnesylated and Carboxymetassociate with the plasma membrane and fulfill its function in cell signaling. We describe here the production of recombinant KRAS4b from our modified baculovirus/insect cell expression system that accurately incorporates these in vivo modifications to allow experiments that anchor KRAS4b to membrane mimetics (e.g., nanodiscs and liposomes).作者: 自由職業(yè)者 時間: 2025-3-27 18:40
Assessment of Plasma Membrane Fatty Acid Composition and Fluidity Using Imaging Flow Cytometrythis chapter, we describe in vitro methods to modulate membrane phospholipid fatty acid composition of cultured cells using fatty acids complexed to bovine serum albumin (BSA). Furthermore, we describe a method to quantify the biophysical properties of plasma membranes in live cells using Di-4-ANEPPDHQ (Di4) and image-based flow cytometry.作者: SPER 時間: 2025-3-27 23:52
Spatiotemporal Imaging of Small GTPase Activity Using Conformational Sensors for GTPase Activity (CO for imaging small GTPase activity in the cell. Conformational changes upon GDP/GTP binding can be visualized directly in solution, on beads, or in live cells using COSGA by fluorescence lifetime imaging microscopy (FLIM) technique. Herein, we describe the construction of COSGA for imaging K-Ras GTPase activity in live cells.作者: Femish 時間: 2025-3-28 05:46
Precise Characterization of KRAS4B Proteoforms by Combining Immunoprecipitation with Top-Down Mass Serization of intact KRAS4B proteoforms. We provide detailed protocols for the IP, LC-MS/MS, and data analysis comprising a successful IP-TDMS assay in the contexts of cancer cell lines and tissue samples.作者: 斷言 時間: 2025-3-28 09:11
NMR Detection Methods for Profiling RAS Nucleotide Cyclingmultiplex these assays, allowing for the precise monitoring of activation states for mixtures of RAS oncoproteins or other RAS superfamily GTPases. Here, we describe the protocols necessary to express and purify isotopically labeled RAS and detail how to carry out an RT-NMR assay on a singular RAS protein or on a mixture of small GTPases.作者: lavish 時間: 2025-3-28 12:43
Ras Diffusion and Interactions with the Plasma Membrane Measured by FRAP Variations the dynamics of the association of N-Ras with the plasma membrane of living cells and their dependence on several parameters (cholesterol, clustering of raft proteins, and palmitoylation/depalmitoylation).作者: 少量 時間: 2025-3-28 17:38
Understanding Ras Spatial Cycles Through Reaction-Diffusion Simulationsreaction networks. This chapter aims to give a brief introduction to setting up reaction-diffusion simulations and applies these in silico techniques to take apart the spatial cycles that maintain Ras localization in the cell.作者: Blood-Vessels 時間: 2025-3-28 22:43
978-1-0716-1192-0Springer Science+Business Media, LLC, part of Springer Nature 2021作者: 歡笑 時間: 2025-3-29 02:44 作者: 釋放 時間: 2025-3-29 04:29 作者: Axon895 時間: 2025-3-29 07:19
Methods to Monitor Ras Activation Staterently used methods, utilizing single- or multi-luminophore-conjugated molecules and external probes. Here we describe methods enabling Ras activity and activation state monitoring in vitro. This chapter focuses mainly on luminescence-based techniques.作者: 單片眼鏡 時間: 2025-3-29 13:58
Ignacio Rubio,Ian PriorIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: oxidant 時間: 2025-3-29 17:21 作者: MUT 時間: 2025-3-29 21:59 作者: 連接 時間: 2025-3-30 02:48 作者: 無能力之人 時間: 2025-3-30 05:56 作者: 推延 時間: 2025-3-30 11:20 作者: 枯燥 時間: 2025-3-30 13:01 作者: 印第安人 時間: 2025-3-30 19:15
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Lauren M. Adams,Caroline J. DeHart,Neil L. Kelleher. . . . . . . . . . . . . . . . . . 79 P . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 273 0 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 108 Q . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 299 E . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 141 R .978-1-4615-9324-9978-1-4615-9322-5
