標(biāo)題: Titlebook: RNAi and Small Regulatory RNAs in Stem Cells; Methods and Protocol Baohong Zhang Book 2017 Springer Science+Business Media, LLC, part of Sp [打印本頁(yè)] 作者: 夾子 時(shí)間: 2025-3-21 17:30
書(shū)目名稱(chēng)RNAi and Small Regulatory RNAs in Stem Cells影響因子(影響力)
書(shū)目名稱(chēng)RNAi and Small Regulatory RNAs in Stem Cells影響因子(影響力)學(xué)科排名
書(shū)目名稱(chēng)RNAi and Small Regulatory RNAs in Stem Cells網(wǎng)絡(luò)公開(kāi)度
書(shū)目名稱(chēng)RNAi and Small Regulatory RNAs in Stem Cells網(wǎng)絡(luò)公開(kāi)度學(xué)科排名
書(shū)目名稱(chēng)RNAi and Small Regulatory RNAs in Stem Cells被引頻次
書(shū)目名稱(chēng)RNAi and Small Regulatory RNAs in Stem Cells被引頻次學(xué)科排名
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書(shū)目名稱(chēng)RNAi and Small Regulatory RNAs in Stem Cells讀者反饋
書(shū)目名稱(chēng)RNAi and Small Regulatory RNAs in Stem Cells讀者反饋學(xué)科排名
作者: antidepressant 時(shí)間: 2025-3-21 23:45
https://doi.org/10.1007/978-1-4939-7108-4Stem cell differentiation; MicroRNAs; RNA interference; CRISPR-Cas9; Regenerative medicine; Gene knockdow作者: Physiatrist 時(shí)間: 2025-3-22 02:56 作者: 背帶 時(shí)間: 2025-3-22 08:35
RNAi and Small Regulatory RNAs in Stem Cells978-1-4939-7108-4Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 一罵死割除 時(shí)間: 2025-3-22 11:53
RNAi and MicroRNA-Mediated Gene Regulation in Stem Cells. In this short review, we give a brief overview of the discovery history, functions, and mechanisms of RNAi and miRNAs. We also discuss the RNAi as a tool to study the stem cell function and the potential future practical applications.作者: figurine 時(shí)間: 2025-3-22 14:07 作者: 確保 時(shí)間: 2025-3-22 19:47 作者: 我要威脅 時(shí)間: 2025-3-22 22:24
Gaoying Ren M.D.,Detlev Boisonor an der Berner Fachhochschule, Hochschule für Technik und Informatik, Biel. Der erste Band besch?ftigt sich mit dem elektrischen Energieversorgungsnetz. Besondere Bedeutung wird den modernen Methoden zur Modellierung der Dynamik der Netzelemente einger?umt.978-3-642-22346-4作者: VICT 時(shí)間: 2025-3-23 02:40
1064-3745 ls.?.Practical and authoritative, .RNAi and Small Regulatory RNAs in Stem Cells: Methods and Protocols. aims to accelerate progress in this crucial field by reducing the time required to decipher and put into practice procedures published in the literature..978-1-4939-8386-5978-1-4939-7108-4Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: POINT 時(shí)間: 2025-3-23 08:37
An Efficient Transfection Method for Differentiation and Cell Proliferation of Mouse Embryonic Stem ucing plasmids encoding the protein; (2) decreased SOX-2 and Oct-3/4 expression and subsequent morphological evidences of cell differentiation after introducing siRNA expression for suppressing SOX-2 and Oct-3/4, which are known to be essential for maintenance of stem cell properties in mouse ES cel作者: 沉思的魚(yú) 時(shí)間: 2025-3-23 13:06
Engineering Human Mesenchymal Stem Cells to Release Adenosine Using miRNA Technologyve passages, and EmGFP-positive hMSC grafts survived in the hippocampal fissure of mouse brains and provided efficient adenosine-dependent neuroprotection in a mouse model of seizure-induced cell loss.作者: 調(diào)整 時(shí)間: 2025-3-23 16:38
Book 2017y reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.?.Practical and authoritative, .RNAi and Small Regulatory RNAs in Stem Cells: Methods and Protocols. aims to accelerate progress in this crucial field by reducing the time required to decipher and put into practice procedures published in the literature..作者: 流利圓滑 時(shí)間: 2025-3-23 19:57 作者: 預(yù)測(cè) 時(shí)間: 2025-3-23 22:42 作者: Recessive 時(shí)間: 2025-3-24 04:54 作者: Estrogen 時(shí)間: 2025-3-24 06:42 作者: 高貴領(lǐng)導(dǎo) 時(shí)間: 2025-3-24 11:20
worden. Darüber hinaus sind die im Buch ben?tigten VDE-Bestimmungen an den neuesten Stand angepasst worden. Bei dieser tiefgeifenden überarbeitung ist wieder strikt darauf geachtet worden, dass das Buch auch weiterhin für ein Selbststudium geeignet ist. 72 Aufgaben mitMusterl?sungen erm?glichem dem Leser ein978-3-663-12081-0作者: 羊齒 時(shí)間: 2025-3-24 15:42 作者: preeclampsia 時(shí)間: 2025-3-24 20:23
Alexander K. Murashov M.D., Ph.D.fasst..Wenn nur station?re Vorg?nge betrachtet werden, verwendet man im technischen Sprachgebrauch anstelle des Begriffes ?Modell“ auch h?ufig den Begriff ?Betriebsverhalten“. Es wird sich zeigen, dass sich das Betriebsverhalten bei einer Reihe von Netzelementen durch galvanisch und induktiv gekoppe作者: Ophthalmologist 時(shí)間: 2025-3-24 23:35
fasst..Wenn nur station?re Vorg?nge betrachtet werden, verwendet man im technischen Sprachgebrauch anstelle des Begriffes ?Modell“ auch h?ufig den Begriff ?Betriebsverhalten“. Es wird sich zeigen, dass sich das Betriebsverhalten bei einer Reihe von Netzelementen durch galvanisch und induktiv gekoppe作者: 愛(ài)國(guó)者 時(shí)間: 2025-3-25 06:41 作者: 舞蹈編排 時(shí)間: 2025-3-25 11:07
Jiao Wang,Qian Wang,Fangfang Zhou,Dong Wang,Tieqiao Wenfasst..Wenn nur station?re Vorg?nge betrachtet werden, verwendet man im technischen Sprachgebrauch anstelle des Begriffes ?Modell“ auch h?ufig den Begriff ?Betriebsverhalten“. Es wird sich zeigen, dass sich das Betriebsverhalten bei einer Reihe von Netzelementen durch galvanisch und induktiv gekoppe作者: reaching 時(shí)間: 2025-3-25 14:02
Carsten Hagemann,Diana Amend,Almuth F. Kessler,Thomas Linsenmann,Ralf-Ingo Ernestus,Mario L?hricht generell bei Studenten nach dem Vorexamen zu erwarten sind, werden erl?utert oder zumindest noch einmal gestreift. Um die Verst?ndlichkeit weiter zu erh?hen, wurden die Modelle, von denen ausgegangen wird, zun?chst sehr einfach gehalten. Für die analytische Formulierung werden nur die Kenntnisse der Math978-3-528-08547-6978-3-322-89730-5作者: Affection 時(shí)間: 2025-3-25 18:29 作者: 遍及 時(shí)間: 2025-3-25 23:51 作者: Commission 時(shí)間: 2025-3-26 02:34
Efficient Depletion of Essential Gene Products for Loss-of-Function Studies in Embryonic Stem CellsER shRNA vector, a high efficiency RNA interference vector, which is based on a 2.5-kb mouse genomic fragment encompassing the H1 gene. We provide here a pHYPER-based protocol optimized to study the function of essential gene products in mouse embryonic stem cells.作者: 老巫婆 時(shí)間: 2025-3-26 04:56 作者: BRUNT 時(shí)間: 2025-3-26 09:06
A Recessive Genetic Screen for Components of the RNA Interference Pathway Performed in Mouse Embryonnd select for RNAi mutants. Putative RNAi mutants were confirmed using a separate functional assay. The location of the gene-trap was then identified using molecular techniques such as splinkerette PCR. Our screening strategy successfully isolated several mutant clones of Argonaute 2, a vital component of the RNAi pathway.作者: 情愛(ài) 時(shí)間: 2025-3-26 13:28
Implanting Glioblastoma Spheroids into Rat Brains and Monitoring Tumor Growth by MRI Volumetryation, and tumor cell invasion into the surrounding brain tissue. In addition, we describe a method for tumor volumetry utilizing the CISS 3D- or contrast-enhanced T1-weighted 3D sequence and freely available post-processing software.作者: LAIR 時(shí)間: 2025-3-26 17:48 作者: 信任 時(shí)間: 2025-3-27 00:35 作者: Axillary 時(shí)間: 2025-3-27 01:11 作者: effrontery 時(shí)間: 2025-3-27 05:45
RNAi and MicroRNA-Mediated Gene Regulation in Stem Cells. In this short review, we give a brief overview of the discovery history, functions, and mechanisms of RNAi and miRNAs. We also discuss the RNAi as a tool to study the stem cell function and the potential future practical applications.作者: 有其法作用 時(shí)間: 2025-3-27 11:42
Forward RNAi Screens in Human Hematopoietic Stem Cellsogy. Here, we describe a method for RNA interference (RNAi)-based screens combined with next-generation sequencing (NGS) in primary human hematopoietic stem and progenitor cells (HSPCs). These cells are suitable targets for complex, selection-based screens using pooled lentiviral short hairpin RNA (作者: indicate 時(shí)間: 2025-3-27 16:43 作者: paradigm 時(shí)間: 2025-3-27 17:50 作者: 精致 時(shí)間: 2025-3-28 01:44 作者: Judicious 時(shí)間: 2025-3-28 03:58
Efficient Depletion of Essential Gene Products for Loss-of-Function Studies in Embryonic Stem Cellss essential for cell survival remains problematic, because genetic ablation of these genes results in cell death. As a consequence, cells recombined at the targeted gene and fully depleted of the gene product cannot be obtained. RNA interference is well suited for the study of essential genes, but t作者: 存心 時(shí)間: 2025-3-28 09:57
Regulation and/or Repression of Cholinergic Differentiation of Murine Embryonic Stem Cells Using RNAene. Regarding differentiation of cells into nervous system components, these techniques would play key roles in elucidating, not only the differentiation mechanisms of neuronal and glial cells but also how neuronal phenotypes are determined. In this chapter, we describe a RNA interference method fo作者: cartilage 時(shí)間: 2025-3-28 11:49
A Recessive Genetic Screen for Components of the RNA Interference Pathway Performed in Mouse Embryonfy components of the mammalian RNAi pathway, we developed a recessive genetic screen in mouse embryonic stem (ES) cells. Recessive genetic screens are feasible in ES cells that are Bloom-syndrome protein deficient (Blm-deficient). We constructed a reporter cell line in Blm-deficient ES cells to isol作者: 寬大 時(shí)間: 2025-3-28 15:51
RNAi Knockdown of Ape1 Gene in the Differentiation of Mouse Embryonic Stem Cellsentiation can be regarded as a valuable biological tool that has led to major advances in our understanding of cell and developmental biology. In vitro differentiation of mouse ES cells can be directed to a specific lineage formation, such as hematopoietic lineage, by appropriate cytokine and/or gro作者: sulcus 時(shí)間: 2025-3-28 20:33 作者: ostensible 時(shí)間: 2025-3-29 02:00
Implanting Glioblastoma Spheroids into Rat Brains and Monitoring Tumor Growth by MRI Volumetryve therapeutical approaches or to analyze the effect of protein overexpression or protein knockdown by RNA interference in vivo, animal models are mandatory. Here, we describe the implantation of C6 glioma spheroids into the rats’ brain and how to follow tumor growth by MRI scans. We show that C6 ce作者: 蹣跚 時(shí)間: 2025-3-29 05:15 作者: 偽造 時(shí)間: 2025-3-29 07:58
In vivo RNAi in the , Follicular Epithelium: Analysis of Stem Cell Maintenance, Proliferation, and D allows to control exactly where and when during development the function of a gene is depleted. The epithelium of the ovary is a particularly good model to study in a living animal how stem cells are maintained and how their descendants proliferate and differentiate. Here I provide basic informatio作者: 恭維 時(shí)間: 2025-3-29 12:41
A Phenotype-Based RNAi Screening for Ras-ERK/MAPK Signaling-Associated Stem Cell Regulators in ,tem cell homeostasis and normal animal development. Particularly, Ras-ERK/MAPK signaling pathway is critical for stem cell self-renewal and differentiation in mammals, including humans. Aberrant regulation of Ras-ERK/MAPK signaling pathway results in either stem cell or overproliferation. Therefore,作者: nominal 時(shí)間: 2025-3-29 19:03
Engineering Human Mesenchymal Stem Cells to Release Adenosine Using miRNA Technologyine-regulating enzyme, is critical to adapt its intra- and extracellular levels in response to environmental changes. Lentiviral RNAi-mediated downregulation of ADK in human mesenchymal stem cells (hMSCs) has therefore been considered an effective tool for engineering therapeutically effective adeno作者: 朝圣者 時(shí)間: 2025-3-29 22:03
Book 2017s. Beginning with a brief introductory section, the volume continues by exploring methods and protocols for RNAi screening, transfection, and the knockdown of specific genes and pathways in several animal species, including humans and mice, recently developed methods for miRNA expression and functio作者: GLUE 時(shí)間: 2025-3-29 23:56 作者: NICE 時(shí)間: 2025-3-30 07:18 作者: HACK 時(shí)間: 2025-3-30 10:38
A Phenotype-Based RNAi Screening for Ras-ERK/MAPK Signaling-Associated Stem Cell Regulators in ,l regulator genes associated with Ras-ERK/MAPK signaling within the context of a whole organism. Importantly, this phenotype-based RNAi screening can be applied for other stem cell-associated signaling pathways such as Wnt/β-catenin and Notch using the ..作者: Generalize 時(shí)間: 2025-3-30 13:51
RNAi Technique in Stem Cell Research: Current Status and Future Perspectivesw of how RNAi suppresses gene expression and to examine some published RNAi approaches that have resulted in changes in stem cell function and suggest the possible clinical relevance of this work in cancer therapy through targeting cancer stem cells.作者: Adulterate 時(shí)間: 2025-3-30 19:22 作者: 甜瓜 時(shí)間: 2025-3-31 00:09
Silencing of ATP11B by RNAi-Induced Changes in Neural Stem Cell Morphologyession of ATP11B, we found distinct morphological changes in neural stem cells. More important, psiRNA-ATP11B-transfected cells displayed short neurite outgrowth compared to the control cells. These data strongly suggest that ATP11B plays a key role in the morphological change of neural stem cells.作者: 教育學(xué) 時(shí)間: 2025-3-31 04:23 作者: 玩忽職守 時(shí)間: 2025-3-31 07:25 作者: 從屬 時(shí)間: 2025-3-31 11:28
Gang-Ming Zoui einem Hersteller oder Betreiber energietechnischer Anlagen t?tig werden wollen. Dementsprechend umfasst dieses Buch die gesamte Breite der elektrischen Energieversorgung. Es wird die Kette von der Energieerzeugung bis hin zu den Verbrauchern behandelt. Den Schwerpunkt bilden die Einrichtungen zum
