標(biāo)題: Titlebook: RNA-Protein Complexes and Interactions; Methods and Protocol Ren-Jang Lin Book 2023Latest edition The Editor(s) (if applicable) and The Aut [打印本頁(yè)] 作者: Coolidge 時(shí)間: 2025-3-21 19:40
書(shū)目名稱RNA-Protein Complexes and Interactions影響因子(影響力)
書(shū)目名稱RNA-Protein Complexes and Interactions影響因子(影響力)學(xué)科排名
書(shū)目名稱RNA-Protein Complexes and Interactions網(wǎng)絡(luò)公開(kāi)度
書(shū)目名稱RNA-Protein Complexes and Interactions網(wǎng)絡(luò)公開(kāi)度學(xué)科排名
書(shū)目名稱RNA-Protein Complexes and Interactions被引頻次
書(shū)目名稱RNA-Protein Complexes and Interactions被引頻次學(xué)科排名
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書(shū)目名稱RNA-Protein Complexes and Interactions年度引用學(xué)科排名
書(shū)目名稱RNA-Protein Complexes and Interactions讀者反饋
書(shū)目名稱RNA-Protein Complexes and Interactions讀者反饋學(xué)科排名
作者: POWER 時(shí)間: 2025-3-21 23:27
Regina T. Nostramo,Anita K. HopperHerausforderungen an die Akustik.Die anspruchsvollen?CO.2.-Ziele??bis 2030/2050 werden unser Mobilit?tsverhalten massiv ver?ndern. Ein wichtiger Beitrag wird von elektrifizierten Fahrzeugantrieben erwartet. Das Buch gibt einen überblick über alle heute diskutierten elektrifizierten Antriebskonzepte 作者: Spina-Bifida 時(shí)間: 2025-3-22 02:31 作者: 共同確定為確 時(shí)間: 2025-3-22 04:44 作者: 大包裹 時(shí)間: 2025-3-22 12:41
Andrzej Chramiec-G??bik,Micha? Rawski,Sebastian Glatt,Ting-Yu Lin作者: 淘氣 時(shí)間: 2025-3-22 14:26
Hironori Adachi,Jonathan L. Chen,Qiangzong Yin,Pedro Morais,Yi-Tao Yu作者: 馬具 時(shí)間: 2025-3-22 18:29 作者: 外露 時(shí)間: 2025-3-22 21:42 作者: ovation 時(shí)間: 2025-3-23 02:05
Identify MicroRNA Targets Using AGO2-CLASH (Cross-linking, Ligation, and Sequencing of Hybrids) and However, because the regulation by miRNAs varies among organs, tissues, cell types and species, identifying relevant targets in specific cells under conditions of interest may not be available. Here, the author describes simplified methods of AGO2-CLASH and AGO2-CLIP to identify miRNA targets by co作者: 一小塊 時(shí)間: 2025-3-23 06:37
In Vitro Reconstitution of Pseudouridylation Catalyzed by Human Box H/ACA Ribonucleoprotein Particlng human cell extracts. We show that a half guide RNA (only one hairpin) is just as functionally competent as the full-length guide RNA (two hairpins) in guiding site-specific pseudouridylation in the human cell extracts. This discovery offers the opportunity for direct delivery of a short guide RNA作者: Gastric 時(shí)間: 2025-3-23 12:38 作者: Aura231 時(shí)間: 2025-3-23 14:17
1064-3745 oritative and cutting-edge,. RNA-Protein Complexes and Interactions: Methods and Protocols, Second Edition .aims to be comprehensive guide for researchers in the field..978-1-0716-3193-5978-1-0716-3191-1Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 加強(qiáng)防衛(wèi) 時(shí)間: 2025-3-23 19:34 作者: Carbon-Monoxide 時(shí)間: 2025-3-24 00:46 作者: 猛烈責(zé)罵 時(shí)間: 2025-3-24 04:46
978-1-0716-3193-5The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines作者: 豐富 時(shí)間: 2025-3-24 09:31 作者: 不吉祥的女人 時(shí)間: 2025-3-24 12:39 作者: 使腐爛 時(shí)間: 2025-3-24 16:19
https://doi.org/10.1007/978-1-0716-3191-1snRNPs; Crispr-Cas9; immunoprecipitation; Mass-spec; Ribo-proteomics作者: Mitigate 時(shí)間: 2025-3-24 19:38
Ren-Jang LinIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: TAG 時(shí)間: 2025-3-25 00:31
A Simple Method for the Detection of Wybutosine-Modified tRNAPheGAA as a Readout of Retrograde tRNAof the movement of tRNAs and the proteins mediating these movements can be difficult. Here, we describe an easy and cost-effective assay to discover genes that are involved in two specific tRNA trafficking events, retrograde nuclear import and nuclear re-export for yeast, .. This assay, referred to 作者: 艱苦地移動(dòng) 時(shí)間: 2025-3-25 05:52
Analysis of Gene Expression Patterns and RNA Localization by Fluorescence in Situ Hybridization in of a method capable of visualizing a single RNA molecule by utilizing tiled fluorescently labeled oligonucleotide probes that together produce a diffraction-limited spot has greatly increased the resolution of this technique, allowing for the precise determination of subcellular RNA localization and作者: 種子 時(shí)間: 2025-3-25 11:24 作者: Heretical 時(shí)間: 2025-3-25 12:44 作者: Lacunar-Stroke 時(shí)間: 2025-3-25 17:07 作者: 感染 時(shí)間: 2025-3-25 20:37
Chemical Probing of RNA Structure In Vivo Using SHAPE-MaP and DMS-MaP, sulfate (DMS) base reactivity can be employed to investigate the flexibility of nucleotides and correlate it to the constraints imparted by base-pairing and/or protein-binding. In vivo, a multitude of proteins could bind an RNA molecule, regulating its structure and function. Hence, to obtain a mor作者: fatuity 時(shí)間: 2025-3-26 02:32 作者: Mammal 時(shí)間: 2025-3-26 08:01
Native RNA Immunoprecipitation (RIP) for Precise Detection and Quantification of Protein-Interactin RNA-binding molecules is called RNA immunoprecipitation (RIP). The RNA precipitated with the studied protein can be detected by real-time polymerase chain reaction (PCR), microarray or sequencing. Here, we detail a method for native immunoprecipitation, without cross-linking, to isolate protein-RNA作者: 小爭(zhēng)吵 時(shí)間: 2025-3-26 12:03 作者: perpetual 時(shí)間: 2025-3-26 16:37 作者: genuine 時(shí)間: 2025-3-26 16:59
Ribosomal Profiling by Gradient Fractionation of Cell Lysates,rful method that is typically employed for monitoring and measuring protein translation status and ribosome activity. Also, it has been used for monitoring the ribosomal stress-responsive events in the ribosome activity. Furthermore, this approach enables understanding of translational regulation, w作者: 網(wǎng)絡(luò)添麻煩 時(shí)間: 2025-3-26 21:30
Global Assessment of Protein Translation in Mammalian Cells Using Polysome Fractionation,ellular translation activity and can be assessed after biochemical fractionations of polysomes. Polysome fractionation begins with immobilizing ribosomes on mRNAs using inhibitors of translation elongation, for example, cycloheximide. Nuclei-free cell lysates are then isolated and layered on the top作者: 偽書(shū) 時(shí)間: 2025-3-27 03:27
,Fluorescent In Situ Detection of RNA–Protein Interactions in Intact Cells by RNA-PLA,ation of a circular DNA template occurs if the target RNA and protein are within 40 nanometers of each other. The resulting circular template is amplified by rolling circle amplification and abundantly recognized by fluorescent antisense DNA oligonucleotides. This strategy therefore enables localiza作者: surrogate 時(shí)間: 2025-3-27 08:28 作者: inflate 時(shí)間: 2025-3-27 11:48
Arresting Spliceosome Intermediates at Various Stages of the Splicing Pathway,tand the molecular mechanism of the splicing reaction, it is necessary to dissect the spliceosome pathway and isolate spliceosome intermediates in various stages of the pathway for biochemical and structural analysis. Here, we describe protocols for preparing intron-containing transcripts, cell-free作者: ADORE 時(shí)間: 2025-3-27 14:52
,Streamlined Purification of RNA–Protein Complexes Using UV Cross-Linking and RNA Antisense Purificadescribe an updated capture method to identify direct and specific RNA–protein interactions. First, RNA and protein are covalently cross-linked in living cells by treatment with UV light at 254 nanometers wavelength. The antisense purification approach is dependent upon nucleic acid hybridization be作者: chalice 時(shí)間: 2025-3-27 19:00
,MS2-MBP-Based Affinity Purification of Nucleus- or Cytoplasm-Localized lncRNA–Protein Complexes Forthe role of lncRNA and its biological function. However, lncRNP purification is still a daunting challenge. Here we describe a protocol to purify lncRNP formed in vivo with MS2-MBP-based affinity purification. Inducible lncRNA tagged with MS2 RNA hairpins is introduced into cells of interest, and RN作者: modifier 時(shí)間: 2025-3-27 22:22
RNA and Protein Interactomes of an RNA-Binding Protein Tagged with FLAG Epitopes Using Combinatory cellular functions, the complete knockout of these proteins may be lethal to the cell. Overexpression of RBPs, on the other hand, may create an altered transcriptome and aberrant phenotypes that can mask their physiological function. Additionally, biochemical characterization of RBP often requires 作者: 雪上輕舟飛過(guò) 時(shí)間: 2025-3-28 05:23
Detecting R-Loop Formation Using a Plasmid-Based In Vitro Transcription Assay,y Ronald Davis and colleagues over 40?years ago, the study of R-loops has become an increasingly expanded area of research. Numerous factors have been identified to modulate the dynamic formation and resolution of R-loops, which are critical for proper controls of gene expression and genome stabilit作者: 吵鬧 時(shí)間: 2025-3-28 07:18
Electrophoretic Mobility Shift Assay (EMSA) and Microscale Thermophoresis (MST) Methods to Measure ious tRNAs toward the homologs of Elp3 from various organisms. The rather qualitative results from EMSA analyses can be nicely complemented by MST measurements allowing precise determination of the dissociation constant (K.). We also provide detailed notes for users to mitigate potential ambiguities and technical pitfalls during the procedures.作者: 舉止粗野的人 時(shí)間: 2025-3-28 13:12
Chemical Probing of RNA Structure In Vivo Using SHAPE-MaP and DMS-MaP, interaction network. In this chapter, we describe methods for characterizing the in vivo nucleotide flexibility of RNA in cells by SHAPE-MaP (SHAPE by .ut.tional .rofiling) and DMS-MaP. In another chapter, we will discuss the characterization of RNA-protein interaction network by RNP-MaP.作者: handle 時(shí)間: 2025-3-28 18:09 作者: concubine 時(shí)間: 2025-3-28 20:46 作者: 古老 時(shí)間: 2025-3-29 02:25 作者: 窗簾等 時(shí)間: 2025-3-29 03:29
,SHAPE to Probe RNA Structure and RNA–Protein Interactions In Vitro,ranscription reaction. The number of RNA molecules with an adduct at a specific nucleotide position indicates the SHAPE reactivity of that nucleotide. Here, we describe the method for probing the structure of an RNA in a protein-free or a protein-bound state by in vitro SHAPE.作者: 藝術(shù) 時(shí)間: 2025-3-29 07:28
Native RNA Immunoprecipitation (RIP) for Precise Detection and Quantification of Protein-Interactinchain reaction (PCR), microarray or sequencing. Here, we detail a method for native immunoprecipitation, without cross-linking, to isolate protein-RNA complexes followed by subsequent extraction and quantification of the co-purified RNA.作者: inspired 時(shí)間: 2025-3-29 13:52
1064-3745 ation advice from the experts.This second edition updates, complements, and expands upon the first edition by providing a collection of cutting-edge techniques developed or refined in the past few years along with tried-and-true methods. Chapters explore the isolation and characterization of RNA-pro作者: 干涉 時(shí)間: 2025-3-29 18:09 作者: CLASP 時(shí)間: 2025-3-29 21:59
A Simple Method for the Detection of Wybutosine-Modified tRNAPheGAA as a Readout of Retrograde tRNAmodified tRNA. serves as a readout of retrograde nuclear import and nuclear re-export. This simple assay can be used to determine the role of any gene product in these previously elusive tRNA trafficking events.作者: meditation 時(shí)間: 2025-3-30 02:31 作者: PALSY 時(shí)間: 2025-3-30 06:03
Global Assessment of Protein Translation in Mammalian Cells Using Polysome Fractionation, gradient and generates the fractions. These fractions can be subjected to further RNA and protein analyses, for example, polysome profiling and mass spectrometry. Here, we present a detailed protocol of polysome fractionation for mammalian cells.作者: flutter 時(shí)間: 2025-3-30 11:59 作者: Spinous-Process 時(shí)間: 2025-3-30 12:56
,Streamlined Purification of RNA–Protein Complexes Using UV Cross-Linking and RNA Antisense Purifica specifically enriched in the target RNA capture. This method has been applied to discover the protein interactions of noncoding RNAs but can be used to capture any RNA where the target sequence is known.作者: 周興旺 時(shí)間: 2025-3-30 17:12
RNA and Protein Interactomes of an RNA-Binding Protein Tagged with FLAG Epitopes Using Combinatory o first stably express an exogenous Flag-tagged RBP and subsequently knockout the endogenous RBP using CRISPR-Cas9 gene editing. The generation of these cell lines will be beneficial for downstream RNA footprinting studies and mass spectrometry-mediated interactome studies.作者: Estrogen 時(shí)間: 2025-3-30 22:29
Analysis of Gene Expression Patterns and RNA Localization by Fluorescence in Situ Hybridization in relative abundance. Here, we present our method for single molecule RNA fluorescence in situ hybridization (smFISH) in whole mount . testes and discuss how we have utilized this method to better understand the expression pattern of the highly unusual Y-linked genes.作者: 網(wǎng)絡(luò)添麻煩 時(shí)間: 2025-3-31 04:56
Analysis of RNA-Protein Interaction Networks Using RNP-MaP,e site of protein-binding. We have discussed methods for chemical probing of structure of the RNA in the protein-free and protein-bound states in the preceding chapters. In this chapter, we describe a ribonucleoprotein mutational profiling (RNP-MaP) method for probing RNA-protein interaction networks.作者: Airtight 時(shí)間: 2025-3-31 08:45 作者: 防止 時(shí)間: 2025-3-31 10:21
The Example of IT System with Fault Tolerance in a Small Business Organizationservices for which reliability must be improved, on the basis of mathematical analysis. This chapter presents methods of improving reliability of web services and databases using the failover Cluster and database mirroring.作者: 品牌 時(shí)間: 2025-3-31 15:15 作者: Cougar 時(shí)間: 2025-3-31 19:06
Book 2017tock markets, tax justice, pension security and the international monetary system - “Bretton Woods II”. Its innovative approach presents several alternatives for each cornerstone, in addition to introducing a participatory democratic process whereby sovereign citizens can themselves determine the ru
