作者: 小口啜飲 時(shí)間: 2025-3-21 22:19 作者: Mhc-Molecule 時(shí)間: 2025-3-22 01:49 作者: Emasculate 時(shí)間: 2025-3-22 05:21
Tuning the Expression of Long Noncoding RNA Loci with CRISPR Interference,CRISPRi has negligible off-target effects and does not involve changes in the underlying genomic DNA sequence, it represents a valuable addition to the existing armamentarium used to investigate lncRNA biology.作者: Overthrow 時(shí)間: 2025-3-22 12:40 作者: 誘拐 時(shí)間: 2025-3-22 14:23 作者: 可轉(zhuǎn)變 時(shí)間: 2025-3-22 19:09 作者: admission 時(shí)間: 2025-3-22 23:13 作者: Calculus 時(shí)間: 2025-3-23 02:26 作者: 無(wú)能力之人 時(shí)間: 2025-3-23 06:29
Tuning the Expression of Long Noncoding RNA Loci with CRISPR Interference,an genome, but only a subset has been involved in regulation of key cellular processes, organismal development, and diseases. Hence, the functional role for the majority of the lncRNA genes remains unknown. With the recent developments of different CRISPR/Cas9 technologies, the function of lncRNAs c作者: scrutiny 時(shí)間: 2025-3-23 11:59
Identification of Chromatin Binding Sites for Long Noncoding RNAs by Chromatin Oligo-Affinity Preciey regulate gene expression. Chromatin oligo-affinity precipitation (ChOP) technique was the first method developed to analyze the association of LncRNAs with genomic regions in the chromatin context. The first step of the procedure is cell cross-linking, aimed at stabilizing the RNA–protein–DNA com作者: 使熄滅 時(shí)間: 2025-3-23 14:02
Knockdown of Nuclear lncRNAs by Locked Nucleic Acid (LNA) Gapmers in Nephron Progenitor Cells,understood. To characterize the function of lncRNAs, knockdown studies are essential. However, the conventional silencing methods for mRNA, such as RNA interference (RNAi), may not be as efficient against lncRNAs, partly due to the mismatch of the localization of lncRNAs and RNAi machinery. To circu作者: 美麗的寫(xiě) 時(shí)間: 2025-3-23 18:23 作者: 災(zāi)禍 時(shí)間: 2025-3-23 22:19 作者: 過(guò)于光澤 時(shí)間: 2025-3-24 05:37
Simultaneous Detection of RNAs and Proteins with Subcellular Resolution,red cells and formal-fixed, paraffin-embedded (FFPE) tissue sections. On cultured cells, we detect specific transcripts using the Stellaris fluorescence in situ hybridization (FISH) probes labeled with fluorophores that target multiple regions along the desired transcripts and proteins combining the作者: Gastric 時(shí)間: 2025-3-24 07:56 作者: Dealing 時(shí)間: 2025-3-24 13:56
Proteomics-Based Systematic Identification of Nuclear Proteins Anchored to Chromatin via RNA,hromatin is likely to be covered with many RNA molecules..Here we describe a simple, reliable, and cross-link-free method for the systematic identification of chromatin-associated RBPs that exhibit RNA-dependent chromatin association.作者: Immortal 時(shí)間: 2025-3-24 18:12 作者: Thymus 時(shí)間: 2025-3-24 21:32 作者: 投票 時(shí)間: 2025-3-25 00:56 作者: 種子 時(shí)間: 2025-3-25 05:12
,Transcriptome-Wide Mapping of Protein–RNA Interactions,e regulation of these processes. The strategy called CLIP-seq (cross-linking and immunoprecipitation) has been developed to capture endogenous protein–RNA interactions with UV cross-linking followed by immunoprecipitation. Despite the wide use of conventional CLIP-seq method in RBP study, the CLIP e作者: 法律 時(shí)間: 2025-3-25 08:02
Proximity RNA-seq : A Sequencing Method to Identify Co-localization of RNA,n which RNA molecules are sequenced in their spatial, cellular context to derive RNA co-localization and transcriptome organization. Transcripts in individual subcellular particles from chemically crosslinked cells are tagged with the same, unique DNA barcode in water-in-oil emulsion droplets. First作者: Emmenagogue 時(shí)間: 2025-3-25 15:42
Characterization of R-Loop Structures Using Single-Molecule R-Loop Footprinting and Sequencing,id and a looped-out single-stranded DNA (ssDNA) strand. These structures are important for normal cellular processes and aberrant R-loop formation has been implicated in a number of pathological outcomes, including certain cancers and neurodegenerative diseases. Mapping R-loops has primarily been pe作者: 滔滔不絕的人 時(shí)間: 2025-3-25 17:13 作者: 加花粗鄙人 時(shí)間: 2025-3-25 20:11
,Analyzing RNA–DNA Triplex Formation in Chromatin,e. Mechanisms of direct and indirect RNA-chromatin interactions have been described, including the sequence-specific formation of triple helix structures. Triplexes are formed by the sequence-specific binding of RNA to the bases located in the major groove of DNA. We recently showed that triplexes d作者: Cerebrovascular 時(shí)間: 2025-3-26 00:57
,Mapping RNA–Chromatin Interactions In Vivo with RNA-DamID,in vivo and many interact with chromatin to regulate gene expression. However, the limited sensitivity of RNA-purification techniques has precluded the identification of genomic targets of cell-type specific lncRNAs. RNA-DamID is a powerful new approach to understand the mechanisms by which lncRNAs 作者: nephritis 時(shí)間: 2025-3-26 05:25 作者: antenna 時(shí)間: 2025-3-26 10:56 作者: sparse 時(shí)間: 2025-3-26 13:12
Book 2020tion and manipulation of gene expression. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubl作者: ARCHE 時(shí)間: 2025-3-26 16:53
Ewan K. S. McRae,David E. Davidson,Sean A. McKennases with solutions make the text suitable for advanced courses or individual study. This completely updated revised new edition contains an enlarged chapter on quantum chromodynamics and a widely updated chapter on neutrino physics..978-3-642-26951-6978-3-642-20241-4Series ISSN 1868-4513 Series E-ISSN 1868-4521 作者: 憤憤不平 時(shí)間: 2025-3-26 22:22 作者: Jocose 時(shí)間: 2025-3-27 03:03 作者: BRINK 時(shí)間: 2025-3-27 09:06 作者: 無(wú)禮回復(fù) 時(shí)間: 2025-3-27 11:34 作者: abstemious 時(shí)間: 2025-3-27 17:04 作者: 雪崩 時(shí)間: 2025-3-27 21:36
t: Stars as Laboratories for Fundamental Physics (Univ. Chicago Press, Chicago 1996) and by H.V. Klapdor-Kleingrothaus and K. Zuber: Particle Astrophysics. 2nd ed. (Inst. of Phys., Bristol 2000), where the basics of this new science can be studied. However, new facts and ideas appear so fast that it作者: ARCHE 時(shí)間: 2025-3-27 21:58 作者: 失眠癥 時(shí)間: 2025-3-28 05:18 作者: parsimony 時(shí)間: 2025-3-28 06:45
Masaki Nishikawa,Norimoto Yanagawald theory is required other than familiarity with effective Lagrangians and Feynmann diagrams. This book will be useful for both theorists and experimentalists who are interested in the phenomenology of electroweak interactions.978-1-4419-2310-3978-0-387-21635-5Series ISSN 0081-3869 Series E-ISSN 1615-0430 作者: 宿醉 時(shí)間: 2025-3-28 10:52 作者: misanthrope 時(shí)間: 2025-3-28 17:30 作者: spinal-stenosis 時(shí)間: 2025-3-28 21:38
Sunjong Kwon,Koei Chin,Michel Nederlof models: The first model is based on the new strong Yukawa dynamics and serves as a platform for studying the ability to reproduce fermion masses. The second, more realistic model introduces a flavor gauge dynamics and its phenomenological consequences are studied..Even though, in the past, this typ作者: incredulity 時(shí)間: 2025-3-29 01:25
Yong-Eun Kim,Kyoon Eon Kim,Kee K. Kimt predicted. One of these is parity violation: The fact that QED conserves parity whereas (bare) CC interactions as well as neutrino induced NC interactions break parity maximally, is introduced into the theory by hand. There is not even a hint at an answer to the question of why right-handed neutri作者: 剝皮 時(shí)間: 2025-3-29 03:34
Kyoko Hiragami-Hamada,Naoki Tani,Jun-ichi Nakayamat predicted. One of these is parity violation: The fact that QED conserves parity whereas (bare) CC interactions as well as neutrino induced NC interactions break parity maximally, is introduced into the theory by hand. There is not even a hint at an answer to the question of why right-handed neutri作者: Wordlist 時(shí)間: 2025-3-29 09:06
Yafei Yin,Xiaohua Sheny. In Sect.?., we show a more dedicated treatment of solving DM density evolution assuming the degenerated parity-odd particles in the system. The derived formulas are necessary to discuss the electroweak-interacting DM system where we have a nearly degenerated parity-odd spectrum.作者: 斗爭(zhēng) 時(shí)間: 2025-3-29 15:06 作者: 邊緣帶來(lái)墨水 時(shí)間: 2025-3-29 19:34 作者: floodgate 時(shí)間: 2025-3-29 21:45 作者: 在前面 時(shí)間: 2025-3-30 03:00 作者: 蠟燭 時(shí)間: 2025-3-30 05:45
galuppi, Caslav Brukner, Jeffrey Bub, Arthur Fine, Christopher Fuchs, GianCarlo Ghirardi, Shelly Goldstein, Daniel Greenberger, Lucien Hardy, Anthony Leggett, Tim Maudlin, David Mermin, Lee Smolin, Antony Valentini, David Wallace, Anton Zeilinger, and Wojciech Zurek. .作者: 滴注 時(shí)間: 2025-3-30 11:18
Targeting Polyadenylation for Retention of RNA at Chromatin,ring with the critical step of transcription termination and 3′end formation provides a means for assaying the functional potential of a given RNA of interest..In this protocol, we describe a method for blocking 3′end processing of the nascent RNA transcript, by using RNase H-inactive antisense olig作者: Initial 時(shí)間: 2025-3-30 12:34 作者: 莊嚴(yán) 時(shí)間: 2025-3-30 19:57 作者: carbohydrate 時(shí)間: 2025-3-30 23:27 作者: 殺子女者 時(shí)間: 2025-3-31 03:58
Characterization of R-Loop Structures Using Single-Molecule R-Loop Footprinting and Sequencing,t relies on the chemical reactivity of the displaced ssDNA strand to non-denaturing sodium bisulfite and single molecule long-read sequencing as a readout, to characterize R-loops. SMRF-seq can be used independently of S9.6 to generate high resolution, strand-specific, maps of individual R-loops at 作者: 瑣碎 時(shí)間: 2025-3-31 06:15 作者: 記憶法 時(shí)間: 2025-3-31 12:58
Lovorka Stojicrity date, when the science manifested itself as a separate and consistent discipline. For particle astrophysics, the science that has developed very - tensively over the last two decades, the birth is most likely to be dated to the beginning of the 1930s. Just then, after the discovery of a neutron作者: NOT 時(shí)間: 2025-3-31 17:13
Marianna Nicoletta Rossi,Rossella Maionerity date, when the science manifested itself as a separate and consistent discipline. For particle astrophysics, the science that has developed very - tensively over the last two decades, the birth is most likely to be dated to the beginning of the 1930s. Just then, after the discovery of a neutron作者: 使顯得不重要 時(shí)間: 2025-3-31 20:15
Masaki Nishikawa,Norimoto Yanagawacs. The effective-theory approach provides the theoretical basis for a model-independent description of the effects sensitive to this mechanism and their impact on observables. This book systematically develops this method, relating the predictions of different physics scenarios in a unified treatme作者: 啞劇 時(shí)間: 2025-3-31 21:42
Célia Carvalho,Maria Carmo-Fonsecacs. The effective-theory approach provides the theoretical basis for a model-independent description of the effects sensitive to this mechanism and their impact on observables. This book systematically develops this method, relating the predictions of different physics scenarios in a unified treatme作者: 大暴雨 時(shí)間: 2025-4-1 05:43 作者: acquisition 時(shí)間: 2025-4-1 08:43
